Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Hanseniaspora thailandica BC9 β-Glucosidase for the Production of β-ᴅ-Hexyl Glucoside

  • Phongprathet, Sujittra (Graduate School, Khon Kaen University) ;
  • Vichitphan, Kanit (Department of Biotechnology and Fermentation Research Center for Value Added Agricultural Products (FerVAAP), Khon Kaen University) ;
  • Han, Jaehong (Metalloenzyme Research Group and Department of Plant Biotechnology, Chung-Ang University) ;
  • Vichitphan, Sukanda (Department of Biotechnology and Fermentation Research Center for Value Added Agricultural Products (FerVAAP), Khon Kaen University) ;
  • Sawaengkaew, Jutaporn (Department of Microbiology, Faculty of Science, Khon Kaen University)
  • Received : 2017.12.15
  • Accepted : 2018.01.18
  • Published : 2018.04.28
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Abstract

For biotechnological production of high-valued ${\beta}-{\text\tiny{D}}$-hexyl glucoside, the catalytic properties of Hanseniaspora thailandica BC9 ${\beta}$-glucosidase purified from the periplasmic fraction were studied, and the transglycosylation activity for the production of ${\beta}-{\text\tiny{D}}$-hexyl glucoside was optimized. The constitutive BC9 ${\beta}$-glucosidase exhibited maximum specific activity at pH 6.0 and $40^{\circ}C$, and the activity of BC9 ${\beta}$-glucosidase was not significantly inhibited by various metal ions. BC9 ${\beta}$-glucosidase did not show a significant activity of cellobiose hydrolysis, but the activity was rather enhanced in the presence of sucrose and medium-chain alcohols. BC9 ${\beta}$-glucosidase exhibited enhanced production of ${\beta}-{\text\tiny{D}}$-hexyl glucoside in the presence of DMSO, and 62% of ${\beta}-{\text\tiny{D}}$-hexyl glucoside conversion was recorded in 4 h in the presence of 5% 1-hexanol and 15% DMSO.

Keywords

References

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