Clinical and Experimental Reproductive Medicine

  • 제45권1호
  • /
  • Pages.10-16
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  • 2018
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
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Effects of selenium on the survival and invasion of trophoblasts

  • Na, Jee Yoon (Cheongshim International Academy) ;
  • Seok, Jin (Department of Biomedical Science, CHA University) ;
  • Park, Sohae (Department of Biomedical Science, CHA University) ;
  • Kim, Jung Seok (Cheongshim International Academy) ;
  • Kim, Gi Jin (Department of Biomedical Science, CHA University)
  • 투고 : 2017.11.05
  • 심사 : 2018.01.23
  • 발행 : 2018.03.31
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초록

Objective: Placental oxidative stress is known to be a factor that contributes to pregnancy failure. The aim of this study was to determine whether selenium could induce antioxidant gene expression and regulate invasive activity and mitochondrial activity in trophoblasts, which are a major cell type of the placenta. Methods: To understand the effects of selenium on trophoblast cells exposed to hypoxia, the viability and invasive activity of trophoblasts were analyzed. The expression of antioxidant enzymes was assessed by reverse-transcription polymerase chain reaction. In addition, the effects of selenium treatment on mitochondrial activity were evaluated in terms of adenosine triphosphate production, mitochondrial membrane potential, and reactive oxygen species levels. Results: Selenium showed positive effects on the viability and migration activity of trophoblast cells when exposed to hypoxia. Interestingly, the increased heme oxygenase 1 expression under hypoxic conditions was decreased by selenium treatment, whereas superoxide dismutase expression was increased in trophoblast cells by selenium treatment for 72 hours, regardless of hypoxia. Selenium-treated trophoblast cells showed increased mitochondrial membrane potential and decreased reactive oxygen species levels under hypoxic conditions for 72 hours. Conclusion: These results will be used as basic data for understanding the mechanism of how trophoblast cells respond to oxidative stress and how selenium promotes the upregulation of related genes and improves the survival rate and invasive ability of trophoblasts through regulating mitochondrial activity. These results suggest that selenium may be used in reproductive medicine for purposes including infertility treatment.

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참고문헌

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  3. Targeting the vascular dysfunction: Potential treatments for preeclampsia vol.26, pp.4, 2019, https://doi.org/10.1111/micc.12522
  4. Evidence for a manifold role of selenium in infertility vol.19, pp.1, 2018, https://doi.org/10.1007/s42000-019-00140-6
  5. Pregnancy, thyroid, and the potential use of selenium vol.19, pp.1, 2020, https://doi.org/10.1007/s42000-019-00144-2
  6. Low Selenium Levels in Amniotic Fluid Correlate with Small-For-Gestational Age Newborns vol.12, pp.10, 2018, https://doi.org/10.3390/nu12103046
  7. Selenium and at-risk pregnancy: challenges and controversies vol.13, pp.1, 2020, https://doi.org/10.1186/s13044-020-00090-x
  8. Hypoxia and Mitochondrial Dysfunction in Pregnancy Complications vol.10, pp.3, 2018, https://doi.org/10.3390/antiox10030405