Figure 1. Phase I and phase II metabolic stability of DC23 in human liver microsomes in the presence of NADPH (A) and UDPGA (B),respectively. Data are the means of triplicate experiments.
Figure 2. Extracted ion chromatograms of DC23 metabolites on microsomal incubation with NADPH and UDPGA.
Figure 3. Product ion scan mass spectra of DC23, its one phase I metabolite (M1), and three phase II metabolites (M2, M3, and M4)obtained by LC-MS/MS analysis following incubation of human liver microsomes with DC23 in the presence of a NADPH andUDPGA.
Figure 4. MS/MS fragmentation schemes for DC23 (A), one phase I metabolite M1 (B), and three phase II metabolites M2-M4 (C). Theoxygen is depicted attached to the 1 position of the triazolothione moiety, for convenience. Our results do not allow determination of theexact oxidation position.
Figure 5. Proposed metabolic pathway of DC23 in human liver microsomes.
Table 1. Inhibitory potency of DC23 on five major cytochromeP450 activities in human liver microsomes.
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