Figure 1. Comparison of full mass spectra at a) 1:0, b) 1:0.1, and c) 1:1 ratios of [peptide]:[Cu] of the active sequences NNQQNY. Allpeaks with m/z values greater than 1000 were magnified by a factor of 20. Magnified NNQQNY [2M+H]1+ peaks are presented asinsets.
Figure 2. MS/MS spectra of singly and doubly charged monomer complexes of NNQQNY, LYQLEN, and VQIVYK peptides with abound Cu(II) ion. [M+Cu-H]1+ of a) NNQQNY, c) LYQLEN, and e) VQIVYK. [M+Cu]2+ of b) NNQQNY, d) LYQLEN, and f)VQIVYK. Peaks labeled -18 likely result from the loss of an H2O moiety. Peaks labeled -28 result from the loss of an additional COmoiety and would conventionally be labeled as an ions. Peaks labeled -44 likely result from the loss of a CO2 moiety. bt fragments areexpressed in blue and bold and yn fragments are expressed in red and bold.
Figure 3. MS/MS spectra of singly and doubly charged dimer complexes of NNQQNY with a bound Cu(II) ion. a) [2M+Cu-H]1+ and b)[2M+Cu]2+. MS/MS/MS spectra of (bt+Cu) 1+ fragments observed in b) spectrum. c) (b5+Cu) 1+ and d) (b4+Cu) 1+.
Table 1. Summary of the nomenclature used for MS/MS spectra. Italics indicate conventional notation as proposed by Roepstorff andFohlman.28 Non-italicized bt would conventionally be represented as a neutral species [bt]0. Non-italicized yn would be represented as[yn”-2H]0.
Table 2. Comparison of [Cu-peptide] MS/MS fragmentationpatterns for NNQQNY, LYQLEN, and VQIVYK.
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