The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Unrecorded Fungal Species Isolated from Indoor Air in the Log Bed- and Sawdust Media-based Mushroom Cultivation Houses

원목 및 톱밥배지 버섯 재배사 내 실내 공기서 분리한 미기록 진균 보고

  • Received : 2018.11.12
  • Accepted : 2018.11.19
  • Published : 2018.12.01
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Abstract

Oak mushroom is cultivated using logs and sawdust media as substrates. In this study, fungi were isolated during a monitoring of indoor air in the oak mushroom cultivation houses located in Cheongyang-gun of Chungnam, Geoje-gun of Gyeongnam, Gumi-si of Gyeongbuk, Jangheung-gun of Jeonnam and Yeoju-si of Gyeongggi-do. Identification of the fungi based on morphology and molecular analysis of the internal transcribed spacer region and 28S rDNA, translation elongation factor translation elongation factor 1 a gene, and ${\beta}-tubulin$ gene revealed that six fungi, Cenangium acuum, Neopestalotiopsis surinamensis, Metarhizium marquandii, Periconia macrospinosa, Trichoderma petersenii, and Trichoderma paratroviride that have not been recorded previously in Korea.

2016년 4월부터 6월까지 표고 원목재배 임가 4곳과 표고 톱밥재배 임가 1곳의 재배사 내 실내 공기에서 진균을 포집 및 분리 동정하였다. 총 6개의 국내 미기록 진균(Cenangium acuum, Periconia macrospinosa, Neopestalotiopsissurinamensis, Metarhiziummarquandii, Trichoderma petersenii, Trichoderma paratroviride)을 분리하였으며 이들 균류에 대하여 형태학적 특성 및 internal transcribed spacer rDNA region, large subunitregion, ${\beta}-tubulin$ 또는 translation elongation factor $1{\alpha}$유전자 염기서열에 기반하여 계통학적 분석 결과를 기술하였다.

Keywords

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Fig. 1. Example of indoor air uptake using air samplers in two different oak mushroom cultivation houses. A, log bed-based mushroom cultivation houses; B, sawdust media-based mushroom cultivation houses.

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Fig. 2. Unrecorded fungal species isolated from mushroom cultivation houses in this study. The fungi were grown on potato dextrose agar at 25℃ for 7 days. A, Cenangium acuum; B, Periconia macrospinosa; C, Neopestalotiopsis surinamensis; D, Metarhizium marquandii; E, Trichoderma petersenii; F, Trichoderma paratroviride.

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Fig. 3. Microscopic image of unrecorded fungal species isolated from mushroom cultivation houses in this study. A, Cenangium acuum; B, Periconia macrospinosa; C, Neopestalotiopsis surinamensis; D, Metarhizium marquandii; E, Trichoderma petersenii; F, Trichoderma paratroviride. Red arrow is important feature (scale bar = 2 μm).

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Fig. 4-2. Phylogenic relationships of the six fungal isolates inferred by the neighbor joining analysis based on 28S rDNA (A), internal transcribed spacer rDNA (B, D), β-tubulin gene (C) or the translation elongation factor 1a gene (E, F) sequences. D, Metarhizium marquandii (NIBRFGC000499882); E, Trichoderma petersenii (NIBRFGC000499883); F, Trichoderma paratroviride (NIBRFGC000499884).

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Fig. 4-2. Phylogenic relationships of the six fungal isolates inferred by the neighbor joining analysisbased on 28S rDNA (A), internal transcribed spacer rDNA (B, D), β-tubulin gene (C) or the translationelongation factor 1a gene (E, F) sequences. D, Metarhizium marquandii (NIBRFGC000499882); E,Trichoderma petersenii (NIBRFGC000499883); F, Trichoderma paratroviride (NIBRFGC000499884).

Table 1. List of the fungal species isolated and identifed in this study from mushroom cultivation houses in this study

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