Production of Anti-dementia Acetylcholinesterase Inhibitors from the Wild Yeasts Saccharomyces cerevisiae WJSL0113 and Wickerhamomyces anomalus JSF0128

Abstract

In this paper, the screening of potent acetylcholinesterase (AChE) inhibitor - producing yeasts from wild yeasts and the condition for the production of anti-dementia AChE inhibitors are described. Among one hundred and seven non-pathogenic wild yeast strains from the waters and soils of three main rivers in Daejeon metropolitan city and midstream of Yeongsangang river in Sangju, sporogenous Saccharomyces cerevisiae WJSL0113 and asporogenous Wickerhamomyces anomalus JSF0128 were selected as useful strains for the production of potent AChE inhibitors. The AChE inhibitors of S. cerevisiae WJSL0113 and W. anomalus JSF0128 had a maximum yield when they were incubated in yeast extract-peptone-dextrose media (pH 6.0 in S. cerevisiae WJSL0113 and pH 5.0 in W. anomalus JSF0128) for 18 hr at $30^{\circ}C$, respectively.

Fig. 1. Phylogenetic tree of anti-dementia acetylcholinesterase inhibitor-producing yeasts, Saccharomyces cerevisiae WJSL0113 and Wickerhamomyces JSF0128, isolated from freshwater based on the nucleotide sequences of large subunit 28S ribosomal DNA. The tree was generated by the neighbor-joining method, using MEGA7

Fig. 2. Effect of cultural time on the acetylcholinesterase inhibitory activity of Saccharomyces cerevisiae WJSL0113 (▭ ) and Wickerhamomyces anomalus JSF0128 (▅)

Fig. 3. Effect of cell-free extract from acetylcholinesterase inhibitor-producing Saccharomyces cerevisiae WJSL0113 on the apoptotic inhibition in pCT105 induced PC12 cell. C (-), not pCT105 induced PC12 cell; C (+), not cell-free extract treated PC12 cell

Table 1. Acetylcholineseterase inhibitory activities on wild yeasts from three main rivers in Daejeon metropolitan city and Yeongsangang river in Sangju city, Korea

Table 2. Characteristics of the selected two wild yeasts, Saccharomyces cerevisiae WJSL0113 and Wickerhamomyces anomalus JSF0128