Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Comparison of Phenolic Acid from Shoots of Aralia elata and Kalopanax pictus Cultivated in Korea Using UPLC-DAD-ESI(+)-QToF/MS

UPLC-DAD-ESI(+)-QToF/MS를 이용한 국내산 두릅나무 및 음나무 순 내 페놀산 특성 비교

  • Kim, Young Jin (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Kim, Heon-Woong (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Lee, Min-Ki (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Lee, Seon-Hye (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Asamenew, Gelila (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Lee, Suji (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Lee, Sang Hoon (Department of Agro-food Resources, National Institute of Agricultural Sciences) ;
  • Cha, Youn-Soo (Department of Food Science and Human Nutrition, College of Human Ecology, Chonbuk National University) ;
  • Kim, Jung Bong (Department of Agro-food Resources, National Institute of Agricultural Sciences)
  • 김영진 (국립농업과학원 농식품자원부 식생활영양과) ;
  • 김헌웅 (국립농업과학원 농식품자원부 식생활영양과) ;
  • 이민기 (국립농업과학원 농식품자원부 식생활영양과) ;
  • 이선혜 (국립농업과학원 농식품자원부 식생활영양과) ;
  • 게릴라 아사메누 (국립농업과학원 농식품자원부 식생활영양과) ;
  • 이수지 (국립농업과학원 농식품자원부 식생활영양과) ;
  • 이상훈 (국립농업과학원 농식품자원부 식생활영양과) ;
  • 차연수 (전북대학교 생활과학대학 식품영양학과) ;
  • 김정봉 (국립농업과학원 농식품자원부 식생활영양과)
  • Received : 2018.10.08
  • Accepted : 2018.11.12
  • Published : 2018.12.31
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Abstract

BACKGROUND: In this study, shoots of Aralia elata and Kalopanax pictus which belong to the Araliaceae family were analyzed using UPLC-DAD-ESI(+)-QToF/MS to characterize of individual phenolic acids. METHODS AND RESULTS: Total thirteen phenolic acids were identified, and nine hydroxycinnamic acid derivatives have been identified for the first time in shoots of Aralia elata and Kalopanax pictus. For total phenolic acid content (mg/100g dry weight), shoots of Aralia elata and Kalopanax pictus showed 754.8 and 845.3 mg/100g, respectively. 5-O-Caffeoylquinic acid (49%) and 3,5-di-O-caffeoylquinic acid (44%) were found as major phenolic acids in Aralia elata, while 5-O-caffeoylquinic acid (91%) was a major component in Kalopanax pictus. CONCLUSION: On comparing the two plants, it was considered that the biosynthesis of 3,5-di-O-caffeoylquinic acid can be affected by 5-O-caffeoylquinic acid in Aralia elata.

UPLC-DAD-ESI(+)-QToF/MS를 사용하여 국내산 두릅나무 및 음나무 순의 페놀산을 분석한 결과 두릅나무 순에서 총 13종, 음나무 순에서 총 8종의 페놀산 성분을 동정하였으며, 내부표준물질을 이용하여 이들 개별 성분을 정량하였다. 개별 페놀산 성분 구조 동정 시 모분자에 $Na^+$ 또는 $K^+$가 결합되는 특징적인 단편이온들이 관찰되어 모분자의 분자량을 쉽게 판단할 수 있었다. 전반적인 구조 동정은 선행연구 및 표준품의 UV, MS 분석 결과를 참고하여 비교하였으며, 특히 이성질체의 효율적인 동정을 위해 SIM mode 방법을 적용하였다. 따라서 두릅나무 및 음나무 순에서 10종(3,4-dihydroxybenzoic acid, 3-O-caffeoylquinic acid, caffeic acid, 4-O-caffeoylquinic acid, 5-O-p-coumaroylquinic acid, 5-O-feruloylquinic acid, 5-O-caffeoylquinic acid methyl ester, 4,5-di-O-caffeoylquinic acid, 3-O-feruloyl-5-O-caffeoylquinic acid, 3-O-caffeoyl-5-O-feruloylquinic acid)의 페놀산이 처음으로 확인되었다. 총 페놀산 함량은 두릅나무 순, 음나무 순이 각각 754.8, 845.3 mg/100g으로 확인하였다. 주요 성분인 5-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid는 두릅나무 순에서 각각 총 페놀산 함량 중 49, 44%로 서로 비슷한 함량을 보인 것이 비해 음나무 순에서는 각각 91, 3%로 확인되었다. 따라서 음나무 순에 비해 두릅나무 순에서 dicaffeoylquinic acid류의 생합성이 활발하게 이루어졌다는 것을 짐작할 수 있었으며, 본 연구 결과는 이들의 생합성에 관여하는 효소, 유전 인자 등을 규명하는데 중요한 기초 자료로 활용될 것으로 사료된다.

Keywords

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