The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
권호 표지
DOI QR코드

DOI QR Code

Quantitative Analysis of Tangshenoside I and Lobetyolin from Korean Deoduk (Codonopsis lanceolata)

국내산 더덕의 Tangshenoside I과 Lobetyolin 정량분석

  • Hwang, Byung Soon (Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Ji Yeong (Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Jang, Mi (Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Gi-Chang (Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Young-Hee (Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Hwang, In Guk (Dept. of Agrofood Resources, National Academy of Agricultural Science, Rural Development Administration)
  • 황병순 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 김지영 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 장미 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 김기창 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 박영희 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 황인국 (농촌진흥청 국립농업과학원 농식품자원부)
  • Received : 2018.10.29
  • Accepted : 2018.11.27
  • Published : 2018.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Deoduk (Codonopsis lanceolata) has a complex chemical composition that includes polyphenols, saponins, amino acids, and other unidentified compounds. The contents of tangshenoside and lobetyolin are considered as standard of quality evaluation of Deoduk. In this study, an ultra-performance liquid chromatography (UPLC) method was developed for the quantitative determination of the two marker constituents, tangshenoside and lobetyolin. The methods for determining the standards of quality were validated by measuring their linearity, specificity, limit of detection (LOD), limit of quantification (LOQ), precision, and accuracy using UPLC. Reversed-phase UPLC analysis was conducted quantitatively to identify individual tangshenoside and lobetyolin in Deoduk extracted with 50% (v/v) aqueous ethanol. We used 21 samples to carry out quantitative analysis of tangshenoside and lobetyolin. Based on their dry weights, the levels of tangshenoside and lobetyolin were 0.36~3.54 mg/g, 0.24~1.29 mg/g, respectively. These results will be valuable as basic data for standardization of Korean Deoduk.

Keywords

HGSPB1_2018_v31n6_957_f0001.png 이미지

Fig. 1. Chemical structures of compounds.

HGSPB1_2018_v31n6_957_f0002.png 이미지

Fig. 2. Tangshenoside I and lobetyolin content of Deoduk by extract condition.

HGSPB1_2018_v31n6_957_f0003.png 이미지

Fig. 3. UPLC chromatogram of standard solution (A) and Deoduk (Codonopsis lanceolata) extract (B).

HGSPB1_2018_v31n6_957_f0004.png 이미지

Fig. 4. Dendrogram for the cluster analysis graph of 21 samples.

Table 1. Validation parameters of the developed UPLC

HGSPB1_2018_v31n6_957_t0001.png 이미지

Table 2. Recovery rates of compounds for the validation of analytical method

HGSPB1_2018_v31n6_957_t0002.png 이미지

Table 3. Contents of standards in Deoduk

HGSPB1_2018_v31n6_957_t0003.png 이미지

References

  1. Cha A, Choi Y, Jin Y, Sung MK, Koo YC, Lee KW, Park T. 2012. Antilipogenic and anti-inflammatory activities of Codonopsis lanceolata in mice hepatic tissues after chronic ethanol feeding. J Biomed Biotechnol 2012:p.141395
  2. Gao SM, Liu JS, Wang M, Cao TT, Qi YD, Zhang BG, Sun XB, Liu HT, Xiao PG. 2018. Traditional uses, phytochemistry, pharmacology and toxicology of Codonopsis: A review. J Ethnopharmacol 219:50-70 https://doi.org/10.1016/j.jep.2018.02.039
  3. He X, Li J, Zhao W, Liu R, Zhang L, Kong X. 2015. Chemical fingerprint analysis for quality control and identification of Ziyang green tea by HPLC. Food Chem 171:405-411 https://doi.org/10.1016/j.foodchem.2014.09.026
  4. Hossen MJ, Kim MY, Kim JH, Cho JY. 2016. Codonopsis lanceolata: A review of its therapeutic potentials. Phytother Res 30:347-356 https://doi.org/10.1002/ptr.5553
  5. Ichikawa M, Ohta S, Komoto N, Ushijima M, Kodera Y, Hayama M, Shirota O, Sekita S, Kuroyanagi M. 2009. Simultaneous determination of seven saponins in the roots of Codonopsis lanceolata by liquid chromatography-mass spectrometry. J Nat Med 63:52-57 https://doi.org/10.1007/s11418-008-0294-4
  6. Jung IH, Jang SE, Joh EH, Chung J, Han MJ, Kim DH. 2012. Lancemaside A isolated from Codonopsis lanceolata and its metabolite echinocystic acid ameliorate scopolamine-induced memory and learning deficits in mice. Phytomedicine 20:84-88 https://doi.org/10.1016/j.phymed.2012.09.005
  7. Kang YH. 2009. Phenolic compounds and antioxidant activity in cell wall materials from Deodeok (Codonopsis lanceolata). Korean J Food Sci Technol 41:345-349
  8. Kim E, Yang WS, Kim JH, Park JG, Kim HG, Ko J, Hong YD, Rho HS, Shin SS, Sung GH, Cho JY. 2014. Lancemaside A from Codonopsis lanceolata modulates the inflammatory responses mediated by monocytes and macrophages. Mediators Inflammationtion 2014p.: 405158
  9. Kim MH, Lee J, Yoo DS, Lee YG, Byeon SE, Hong EK, Cho JY. 2009. Protective effect of stress-induced liver damage by saponin fraction from Codonopsis lanceolata. Arch Pharmacal Res 32:1441-1446 https://doi.org/10.1007/s12272-009-2014-3
  10. Kim OK. 2016. Antidiabetic effect of ethanol extract on Codonopsis lanceolata root in streptozotocin induced diabetic rats. J Korean Oil Chemists Soc 33:271-277 https://doi.org/10.12925/jkocs.2016.33.2.271
  11. Kim SH, Chung MJ, Jang HD, Ham SS. 2010. Antioxidative activities of the Codonopsis lanceolata extract in vitro and in vivo. J Korean Soc Food Sci Nutr 39:193-202 https://doi.org/10.3746/jkfn.2010.39.2.193
  12. Ko SC, Son DC, Kim HJ, Hwang HS, Shin YH. 2009. Flora of Wonju-Hoengseong area. Korean J Plant Res 22:365-380
  13. Korea Forest Service. 2018. Production of Forest Products. pp.31, 48
  14. Lee KW, Jung HJ, Park HJ, Kim DG, Lee JY, Lee KT. 2005. Beta-D-Xylopyranosyl-(1-3)-beta-D-glucuronopyranosyl echinocystic acid isolated from the roots of Codonopsis lanceolata induces caspase-dependent apoptosis in human acute promyelocytic leukemia HL-60 cells. Biol Pharm Bull 28:854-859 https://doi.org/10.1248/bpb.28.854
  15. Lee LS, Kim SH, Park JD, Kim YB, Kim YC. 2015. Physicochemical properties and antioxidant activities of loose-leaf green tea commercially available in Korea. Korean J Food Sci Technol 47:419-424 https://doi.org/10.9721/KJFST.2015.47.4.419
  16. Lee SH, Choi HJ, Heo JC, Lee JH, Kwon TK, Ha SC, Lee SH. 2017. Comparison of anti-asthmatic activity by native Codonopsis lanceolata extract. J Life Sci 27:450-455 https://doi.org/10.5352/JLS.2017.27.4.450
  17. Lee YG, Kim JY, Lee JY, Byeon SE, Hong EK, Lee J, Rhee MH, Park HJ, Cho JY. 2007. Regulatory effects of Codonopsis lanceolata on macrophage-mediated immune responses. J Ethnopharmacol 112:180-188 https://doi.org/10.1016/j.jep.2007.02.026
  18. Li JP, Liang ZM, Yuan Z. 2007. Triterpenoid saponins and antiinflammatory activity of Codonopsis lanceolata. Pharmazie 62:463-466
  19. Li XS, Kuto S, Kanazawa I. 2007. Biochemical effects of Codonopsis lanceolata extract. West China J Pharm Sci 22:306
  20. Park SJ, Park DS, Yoon WB, He X, Ahn JH. 2012. Effects of aged Deodeok extract on serum lipid content in rats fed a high-fat diet. J East Asian Soc Diet Life 22:17-24
  21. Park SJ, Seong DH, Park DS, Kim SS, Gou J, Ahn JH, Yoon WB, Lee HY. 2009. Chemical compositions of fermented Codonopsis lanceolata. J Korean Soc Food Sci Nutr 38:396-400 https://doi.org/10.3746/jkfn.2009.38.3.396
  22. Park SJ, Seong DH, Park DS, Kim SS, Gou J, Ahn JH, Yoon WB, Lee HY. 2009. Chemical compositions of fermented Codonopsis lanceolata. J Korean Soc Food Sci Nutr 38:983-988 https://doi.org/10.3746/jkfn.2009.38.8.983
  23. Ren J, Lin Z, Yuan Z. 2013. Tangshenosides from Codonopsis lanceolata roots. Phytochem Lett 6:567-569 https://doi.org/10.1016/j.phytol.2013.07.008
  24. Ryu HS. 2008. Effects of Codonopsis lanceolata extracts on mouse immune cell activation. Korean J Food Nutr 21:263-268
  25. Ryu HS. 2009. Effect of Codonopsis lanceolata extracts on mouse IL-2, IFN-${\gamma}$, IL-10 cytokine production by peritoneal macrophage and the ratio of IFN-${\gamma}$, IL-10 cytokine. Korean J Food Nutr 22:69-74
  26. Wang H, Helliwell K. 2000. Epimerisation of catechins in green tea infusions. Food Chem 70:337-344 https://doi.org/10.1016/S0308-8146(00)00099-6
  27. Wang L, Xu ML, Hu JH, Rasmussen SK, Wang MH. 2011. Codonopsis lanceolata extract induces G0/G1 arrest and apoptosis in human colon tumor HT-29 cells involvement of ROS generation and polyamine depletion. Food Chem Toxicol 49:149-154 https://doi.org/10.1016/j.fct.2010.10.010
  28. Weon JB, Lee J, Eom MR, Jung YS, Ko HJ, Lee HY, Park DS, Chung HC, Chung JY, Ma CJ. 2014. Effect of water and ethanol extracts Codonopsis lanceolata on spatial learning and memory in mice. Yakhak Hoeji 58:287-293
  29. Weon JB, Yun BR, Lee J, Eom MR, Ko HJ, Lee HY, Park DS, Chung HC, Chung JY, Ma CJ. 2014. Effect of steamed Codonopsis lanceolata on spatial learning and memory in mice. Kor J Pharmacogn 45:48-54
  30. Xia Y, Liu F, Feng F, Liu W. 2017. Characterization, quantitation and similarity evaluation of Codonopsis lanceolata from different regions in China by HPLC-Q-TQF-MS and chemometrics. J Food Compos Anal 62:134-142 https://doi.org/10.1016/j.jfca.2017.05.009
  31. Xu LP, Wang H, Yuan Z. 2008. Triterpenoid saponins with antiinflammatory activity from Codonopsis lanceolata. Planta Med 74:1412-1415 https://doi.org/10.1055/s-2008-1081318
  32. Zhao B, Ren J, Yuan Z. 2013. Isolation of a new cerebroside from Codonopsis lanceolata. Biochem Syst Ecol 46:26-28 https://doi.org/10.1016/j.bse.2012.09.004