The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Immune Enhancing Effect by Ethanol Extract of Ailantias altissima

가죽나무 에탄올 추출물에 의한 면역증강 효과

  • Gil, Na-Young (Fermented and Processed Food Science Division, National Institute of Agricultural Science, RDA) ;
  • Kim, Sun-Hee (Fermented and Processed Food Science Division, National Institute of Agricultural Science, RDA) ;
  • Choi, Bo-Young (Fermented and Processed Food Science Division, National Institute of Agricultural Science, RDA) ;
  • Mun, Ji-Young (Fermented and Processed Food Science Division, National Institute of Agricultural Science, RDA) ;
  • Yeo, Soo-Hwan (Fermented and Processed Food Science Division, National Institute of Agricultural Science, RDA) ;
  • Kim, So-Young (Fermented and Processed Food Science Division, National Institute of Agricultural Science, RDA)
  • 길나영 (농촌진흥청 국립농업과학원 발효가공식품과) ;
  • 김순희 (농촌진흥청 국립농업과학원 발효가공식품과) ;
  • 최보영 (농촌진흥청 국립농업과학원 발효가공식품과) ;
  • 문지영 (농촌진흥청 국립농업과학원 발효가공식품과) ;
  • 여수환 (농촌진흥청 국립농업과학원 발효가공식품과) ;
  • 김소영 (농촌진흥청 국립농업과학원 발효가공식품과)
  • Received : 2018.11.24
  • Accepted : 2018.12.13
  • Published : 2018.12.31
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Abstract

The aim of this study was to investigate the immune activity of Ailantias altissima as an active ingredient on the immune enhancement by mixing ethanol extract with lactic acid bacteria (LAB). The activity of $NF-{\kappa}B/AP1$ transcription factor increased by $NF-{\kappa}B$ activity when mixed with LAB samples rather than with the extract alone. Nitric oxide (NO) production was similar in ethanol extract alone group and LPS treatment group. Mixing Ailantias altissima extract and lactic acid bacteria led to low NO production. The cytokine productivity of $TNF-{\alpha}$ significantly increased in Ailantias altissima extract when treated with LPS, and increased even more when mixed with lactic acid bacteria. The $IL-1{\beta}$ cytokine production was high when the Ailantias altissima extract were treated alone, but no $IL-1{\beta}$ cytokine was produced in the mixtures with isolates. The combination of the ethanol extract of the Ailantias altissima and the lactic acid bacteria was found to be effective in the immune function. Consequently, the ingredient to combine Ailantias altissima extract and lactic acid bacteria can be effectively used for development of the health functional food on the prevention and treatment of hypoimmunities.

Keywords

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Fig. 1. Effect of Ailantias altissima ethanol extract on cell viability in RAW-BLUE cells.

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Fig. 2. Effects of Ailantias altissima extract & mixing the ethanol extract and lactic acid bacteria (live probiotics) on the production of NF-κB and NO in RAW-BlueTM cell.

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Fig. 3. Effects of Ailantias altissima extract & mixing the ethanol extract and lactic acid bacteria (live probiotics) on the production of cytokines (TNF-α, IL-1β) in RAW-BlueTM cell.

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Fig. 4. Effects of Ailantias altissima extract & mixing the ethanol extract and lactic acid bacteria (killed probiotics) on the production of NF-κB and NO in RAW-BlueTM cell.

HGSPB1_2018_v31n6_940_f0005.png 이미지

Fig. 5. Effects of Ailantias altissima extract & mixing the ethanol extract and lactic acid bacteria (killed probiotics) on the production of cytokines (TNF-α, IL-1β) in RAW-BlueTM cell.

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