The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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A Study of Effects of Crataegus pinnatifida var. psilosa Extracts

좁은잎산사나무 열매 추출물의 효능에 관한 연구

  • 이광수 (장안대학교 건강과학부 식품영양과) ;
  • 박경숙 (장안대학교 건강과학부 식품영양과)
  • Received : 2018.10.01
  • Accepted : 2018.10.25
  • Published : 2018.12.31
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Abstract

The purpose of this study was to investigate effects of Crataegus pinnatifida var. psilosa extracts obtained from 95% methanol and water. Methylene chloride, ethyl acetate, and methanol were used to fractionate the 95% methanol extract and each fraction was testified total polyphenol contents, electron donating abilities, the scavenging abilities of superoxide anion radical, as well as hydrogen peroxide. Extraction yields of 95% methanol and water from Crataegus pinnatifida var. psilosa were 25.40%, 23.12% respectively. Total polyphenol contents were $28,708.0{\pm}1,755.05{\mu}g\;GAE/mL$ in 95% methanol, revealing the highest among them, $12,726.67{\pm}479.33{\mu}g\;GAE/mL$ in water extract, $15,854.67{\pm}498.38{\mu}g\;GAE/mL$ in methanol fraction, $11,810.67{\pm}584.48{\mu}g\;GAE/mL$ in ethyl acetate fraction, and $5294.67{\pm}190.36{\mu}g\;GAE/mL$ in methylene chloride fraction. Total polyphenol contents revealed significant differences (p<0.05) between the solvents. In the experiment of the electron donating ability, water extract revealed $84.33{\pm}0.1%$ scavenging ability, the highest. Other extracts and fractions were $81.8{\pm}1.11%$ for water, $79.73{\pm}1.32%$ for ethyl acetate fraction, $75.73{\pm}2.17%$ for methylene chloride fraction, and $42.1{\pm}5.01%$ for methanol fraction, the lowest electron donating ability. Electron donating abilities revealed significant difference (p<0.05) between the solvents. In the experiment of superoxide anion radical scavenging ability, ethyl acetate fraction($0.0026{\pm}0.0002$) had the highest scavenging ability, and the others revealed slight increase rather than decrease in scavenging ability. Hydrogen peroxide scavenging ability revealed the highest in methanol fraction ($-0.00206{\pm}0.00165$) and the others were as follow; water extract ($0.00157{\pm}0.00249$), 95.0% methanol extract ($0.005{\pm}0.0036$), methylene chloride fraction ($0.0039{\pm}0.00364$), and ethyl acetate fraction ($0.0002{\pm}0.00059$).

Keywords

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Fig. 1. The measurement of superoxide anion radical depending on fractions.

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Fig. 2. The measurement of hydrogen peroxide decomposition depending on fractions.

Table 1. The total polyphenol contents obtained from C. pinnatifida var. psilosa extracts using different solvents

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Table 2. Electron donating ability of C. pinnatifida var. psilosa extracts obtained from different solvents

HGSPB1_2018_v31n6_775_t0002.png 이미지

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