Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Effects of Hypochlorous Acid, Calcium Chloride and Phosphoric Acid in a Highly Saline Solution on Cell Death Rate and Growth Rate of Porphyra yezoensis and Ulva intestinalis

고염수 처리제에 차아염소산, 염화칼슘 및 인산 첨가가 방사무늬김(Pyropia yezoensis)과 창자파래(Ulva intestinalis)의 사세포율과 엽체 성장에 미치는 영향

  • Kwon, O-Nam (East Coastal Life Science Institute, Gangneung-Wonju National University) ;
  • Yun, Yeoung-Rang (Honam South-Eastern Laver Complex Solution Business Cooperation) ;
  • Shin, Il-Shik (Department of Marine Food Science and Technology, Gangneung-Wonju National University)
  • 권오남 (강릉원주대학교 동해안생명과학연구소) ;
  • 윤영랑 (호남남서부김활성처리제사업협동조합) ;
  • 신일식 (강릉원주대학교 해양식품공학과)
  • Received : 2018.11.20
  • Accepted : 2018.12.11
  • Published : 2018.12.31
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Abstract

We investigated the effects of a highly saline solution (HS) containing hypochlorous acid, calcium chloride ($CaCl_2$), and phosphoric acid ($H_3PO_4$) on cell death and growth rate of laver Porphyra yezoensis and green laver Ulva intestinalis. Cell death rates of laver treated with HS and HS plus hypochlorous acid (HS + HOCl) in the harvesting stage were less than 0.5%, and there were no significant differences between the HS and HS + HOCl treatments. However, cell death of green laver treated with HS + HOCl in the harvesting stage was greater than 81.2%. These results indicate that the addition of HOCl is highly effective to eradicate noxious green laver without causing damage to laver. The addition of HOCl and $H_3PO_4$ to HS did not increase the area or weight of laver blades. A combination treatment of $CaCl_2$ and HS, however, significantly increased the area and weight of laver lades compared to controls (P<0.05).

Keywords

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Fig. 1. Change of Laver Pyropia yezoensis and Green laver Ulva intestinalis cells by treatment with HS+HOCl on early and harveststage of Laver. HS, High salty solution.

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Fig. 2. The death cell ratio of Laver Pyropia yezoensis and Green laver Ulva intestinalis by treatment with HS and HS+HOCl atearly and harvest stage of laver. Values with different characters are signifcantly different (P<0.05). (a) early stage of laver; (b)harvest stage of laver; (c) early stage of green laver; (d) harvest stage of green Laver. HS, High salty solution.

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Fig. 3. The effects of addition of HOCl, CaCl2 and H3PO4 to HS on increase of Laver Pyropia yezoensis blade area. Values with different characters are significantly different (P<0.05). HS, High salty solution.

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Fig. 4. The effects of addition of HOCl, CaCl2 and H3PO4 to HS on increase of Laver Pyropia yezoensis blade weight. Values with different characters are significantly different (P<0.05). HS, High salty solution.

Table 1. Composition (%) of high salty complex solution used for measurement of death cell ratio of Laver Pyropia yezoensis and Green laver Ulva intestinalis blade

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Table 2. Composition (%) of high salty complex solutions used for measurement of growth rate of Laver Pyropia yezoensis leaves in this study

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