Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Fission yeast Pci2 has function in mRNA export as a component of TREX-2

분열효모 Pci2가 TREX-2 구성요소로서 mRNA 방출에 미치는 영향

  • Park, Jin Hee (Department of Biotechnology, Sungshin Women's University) ;
  • Yoon, Jin Ho (Department of Biotechnology, Sungshin Women's University)
  • 박진희 (성신여자대학교 바이오생명공학과) ;
  • 윤진호 (성신여자대학교 바이오생명공학과)
  • Received : 2018.11.26
  • Accepted : 2018.12.05
  • Published : 2018.12.31
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Abstract

Thp1/PCID2, PCI domain-containing protein, is a component of the evolutionally conserved TREX-2 complex linking mRNA transcription and export. In fission yeast, Schizosaccharomyces pombe, the pci2 (SPBC1105.07c) gene encodes a PCI domain-containing protein that is predicted as a fission yeast orthologue of Thp1 (in budding yeast)/PCID2 (in human). Repression of pci2 expression inhibited both growth and mRNA export. And over-expression of pci2 also exhibited growth retardation with slight accumulation of $poly(A)^+$ RNA in the nucleus. Moreover, yeast two-hybrid and co-immunoprecipitation analysis showed that the Pci2 protein physically interacted with Sac3 and Dss1, which are members of TREX-2 complex. These observations support that the S. pombe Pci2 protein, as a component of TREX-2 complex, is implicated in mRNA export.

PCI 영역을 포함하고 있는 Thp1/PCID2 단백질은 mRNA 전사와 방출을 연결하는, 진화적으로 보존된 TREX-2 복합체의 구성요소이다. 분열효모인 Schizosaccharomyces pombe에서 pci2 (SPBC1105.07c) 유전자는 TREX-2 복합체의 구성요소인 Thp1 (출아효모)/PCID2 (사람)의 분열효모 이종상동체로 추정되는 PCI 영역을 갖는 단백질을 암호화하고 있다. pci2 발현을 억제하면 생장과 mRNA 방출이 모두 억제되었다. 그리고 pci2 유전자의 과발현 또한 생장을 늦추고 $poly(A)^+$ RNA를 핵 안에 약간 축적되게 하였다. 뿐만 아니라 Yeast two-hybrid와 공동침전(Co-immunoprecipitation) 분석 실험에서 Pci2는 TREX-2 복합체의 다른 구성요소인 Sac3, Dss1 단백질들과 물리적으로 상호작용하였다. 이와 같은 관찰들은 S. pombe의 Pci2 단백질도 TREX-2 복합체의 구성요소로서 mRNA 방출에 관여함을 의미한다.

Keywords

MSMHBQ_2018_v54n4_325_f0001.png 이미지

Fig. 1. S. pombe pci2 is crucial for growth and mRNA export.

MSMHBQ_2018_v54n4_325_f0002.png 이미지

Fig. 2. Over-expression of pci2 cause slight growth retardation and the defects in mRNA export.

MSMHBQ_2018_v54n4_325_f0003.png 이미지

Fig. 3. Physical interaction of Pci2 with Sac3 in S. pombe extracts.

Table 1. Yeast two-hybrid analysis

MSMHBQ_2018_v54n4_325_t0001.png 이미지

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