Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Comparative Study on the Effects of Six Species of Microalgae Extracts on Hair Loss Prevention and Scalp Improvement

6종의 미세조류 추출물의 탈모예방 및 두피 개선 효과 비교

  • 정민화 (대동대학교 헤어디자인과) ;
  • 이수선 ((주)선마린바이오테크) ;
  • 김현아 ((주)선마린바이오테크) ;
  • 황혜정 (동부산대학교 식품영양학과)
  • Received : 2017.10.24
  • Accepted : 2018.01.31
  • Published : 2018.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study examines the role of six species of microalgae, including Phaeodacylum tricrnutum (PT), Chaetoceros gracilis (CG), Nanochloris oculata (NO), Pavlova lutheri (PL), Chlorella ellipsoidae (CE), and Scendedemus obliquus (SO), on hair loss prevention and scalp improvement. To determine the effects of microalgae extracts on hair loss prevention and scalp improvement, antioxidant activity, cell proliferation in HaCaT cells and HFDPC cells, and the inhibition level of 5-alpha reductase activity were examined. In the study of antioxidant activity, the $EC_{50}$ values of DPPH anti-radical activities indicated that the SO, CG, and ST9 treatment groups demonstrate significant antioxidant activity. In the study of the hydroxyl radical scavenging activity, CG (6.6~42.1%), ST9 (26.0~44.0%), and SO (7.8~44.3%) demonstrated significant effects. Furthermore, SO promoted the proliferation of HaCaT cells and a human epidermal cell line during a 6-day treatment. In the study of the proliferation of HFDPC cells, a hair follicle dermal papilla cell line, CG, and SO significantly stimulated cell proliferation. Finally, PT, CG, and SO significantly inhibited 5-alpha reductase activity. These results suggest that among the six microalgae used in this study, CG and SO have antioxidant effects, induce cell proliferation, inhibit 5-alpha reductase activity, and can be used for hair loss prevention and scalp improvement.

이번 연구에서 우리는 6종의 미세조류 ; Phaeodacylum tricrnutum (PT), Chaetoceros gracilis (CG), Nanochloris oculata (NO), Pavlova lutheri (PL), Chlorella ellipsoidae (CE), and Scendedemus obliquus (SO)를 이용하여 탈모 예방 및 두피 개선효과를 살펴보았다. DPPH 라디칼 소거능 실험에서는 SO, CG, ST9 순으로 활성이 높은 것으로 나타났다. OH 소거능 실험 결과, CG (6.6~42.1%), ST9 (26.0~44.0%), SO (7.8~44.3%)으로 유의적인 효과를 보였다. 그외에도 세포증식실험에서는, SO가 인간표피세포주인 HaCaT 세포 증식을 촉진시켰으며, CG와 SO가 모유두세포주인 HFDPC 세포 증식을 촉진시키는 것으로 나타났다. 마지막으로 5-alpha reductase 저해능 실험결과, PT, CG, SO가 유의적으로 효소활성을 저해하였다. 따라서, 이번 연구에 사용한 6종의 미세조류들 중, 항산화활성, 세포증식 효과, 5-alpha reductase 저해능을 보인 몇가지 조류들이 탈모예방 및 두피개선을 위한 제품개발에 좋은 원료가 될 가능성이 있음을 제시한다.

Keywords

References

  1. Chew, K. W., Yap, J. Y., Show, P. L., Suan, N. H., Juan, J. C., Ling, T. C., Lee, D. J. and Chang, J. S. 2017. Microalgae biorefinery: High value products perspectives. Bioresour. Technol. 229, 53-62. https://doi.org/10.1016/j.biortech.2017.01.006
  2. Dahms, H. U. and Dobretsov, S. 2017. Antifouling compounds from marine macroalgae. Mar. Drugs 15, pii: E265. doi: 10.3390/md15090265.
  3. Guldhe, A., Kumari, S., Ramanna, L., Ramsundar, P., Singh, P., Rawat, I. and Bux, F. 2017. Prospects, recent advancements and challenges of different wastewater streams for microalgal cultivation. J. Environ. Manage. 203, 299-315. https://doi.org/10.1016/j.jenvman.2017.08.012
  4. Henriquez, V., Escobar, C., Galarza, J. and Gimpel, J. 2016. Carotenoids in microalgae. Subcell Biochem. 79, 219-237.
  5. Hong, Y. H., Bae, S. H. and Seo, H. J. 2015. Effect of herbal complex extract including houttuynia cordata thumb on hair growth promotion in C57BL/6 mice. Kor. J. Aesthet. Cosmetol. 13, 321-329.
  6. Jesus Raposo, M. F., Morais, A. M. and Morais, R. M. 2015. Marine polysaccharides from algae with potential biomedical applications. Mar. Drugs 13, 2967-3028. https://doi.org/10.3390/md13052967
  7. Kim, G. S. 2014. Research trends relevant with hair growth promotion and scalp condition improvement by applying natural extracts. Kor. J. Aesthet. Cosmetol. 12, 17-24.
  8. Kim, M. H., Choi, Y. Y., Cho, I. H., Hong, J., Kim, S. H. and Yang, W. M. 2014. Angelica sinensis induces hair regrowth via the inhibition of apoptosis signaling. Am. J. Chin. Med. 42, 1021-1034. https://doi.org/10.1142/S0192415X14500645
  9. Moreno-Arrones, O. M., Becerra, A. and Vano-Galvan, S. 2017. Therapeutic experience with oral finasteride for androgenetic alopecia in female-to-male transgender patients. Clin. Exp. Dermatol. 42, 743-748. https://doi.org/10.1111/ced.13184
  10. Park, P. J., Heo, S. J., Park, E. J., Kim, S. K., Byun, H. G., Jeon, B. T. and Jeon, Y. J. 2005. Reactive oxygen scavenging effect of enzymatic extracts from Sargassum thunbergii. J. Agric. Food. Chem. 53, 6666-6672. https://doi.org/10.1021/jf050582+
  11. Patel, S., Sharma, V., Chauhan, N. S., Thakur, M. and Dixit, V. K. 2015. Hair growth: focus on herbal therapeutic agent. Curr. Drug Discov. Technol. 12, 21-42. https://doi.org/10.2174/1570163812666150610115055
  12. Patro, G., Bhattamisra, S. K., Mohanty, B. K. and Sahoo, H. B. 2016. In vitro and in vivo antioxidant evaluation and estimation of total phenolic, flavonoidal content of mimosa pudica L. Pharmacognosy Res. 8, 22-28.
  13. Phillips, T. G., Slomiany, W. P. and Allison, R. 2017. Hair loss: common causes and treatment. Am. Fam. Physician 15, 371-378.
  14. Piraccini, B. M. and Alessandrini, A. 2014. Androgenetic alopecia. G. Ital. Dermatol. Venereol. 149, 15-24.
  15. Purba, T. S., Brunken, L., Peake, M., Shahmalak, A., Chaves, A., Poblet, E., Ceballos, L., Gandarillas, A. and Paus, R. 2017. Characterisation of cell cycle arrest and terminal differentiation in a maximally proliferative human epithelial tissue: Lessons from the human hair follicle matrix. Eur. J. Cell Biol. 96, 632-641. https://doi.org/10.1016/j.ejcb.2017.03.011
  16. Shah, K. B., Shah, A. N., Solanki, R. B. and Raval, R. C. 2017. A comparative study of microneedling with platelet- rich plasma plus topical minoxidil (5%) and topical minoxidil (5%) alone in androgenetic alopecia. Int. J. Trichol. 9, 14-18.
  17. Talavera-Adame, D., Newman, D. and Newman, N. 2017. Conventional and novel stem cell based therapies for androgenic alopecia. Stem Cells Cloning 31, 11-19.
  18. Wu, J. H. and Sun, Z. Y. 2013. Establishment of an in vitro screening model for steroid 5 alpha-reductase inhibitors with the microplate reader. Zhonghua Nan Ke Xue. 19, 483-486.
  19. Wu, Q. Y., Wu, X. J., Lu, Z. F. and Zheng, M. 2006. Effects of traditional Chinese herbs on growth of mouse hair follicles and hair bulb cells in vitro. Zhejiang Da Xue Xue Bao Yi Xue Ban. 35, 435-439.
  20. Wu, Y., Lin, Y., Liu, H. J., Huang, C. Z., Feng, A. P. and Li, J. W. 2010. Childhood psoriasis: a study of 137 cases from central China. World J. Pediatr. 6, 260-264. https://doi.org/10.1007/s12519-010-0213-0
  21. Yadav, M. K., Choi ,J. and Song, J. J. 2014. Protective effect of hexane and ethanol extract of piper longum L. On gentamicin- induced hair cell loss in neonatal cultures. Clin. Exp. Otorhinolaryngol. 7, 13-18. https://doi.org/10.3342/ceo.2014.7.1.13