Journal of Digital Convergence (디지털융복합연구)

The Society of Digital Policy and Management (한국디지털정책학회)
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Anti-inflammatory activity Effects of Mori Folium Water Extracton IL-1α, IL-6 and IL-10 on mouse macrophages

상엽 추출물이 마우스 대식세포의 IL-1α, IL-6, IL-10에 대한 항염활성 연구

  • Park, Young Sik (Department of Alternative medicine, Nambu University) ;
  • Han, Hyo-Sang (Department of Health Administration, Joongbu University)
  • Received : 2018.09.19
  • Accepted : 2018.11.20
  • Published : 2018.11.28
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Abstract

This study was conducted to investigate of Mori Folium Water Extract (MF) on anti-inflammation activity. MF Water extracts after 24 houres cultivation were examined to ascertain the cell viability of mouse macrophage RAW 264.7 cells. The influence of the Water extracts in RAW 264.7 macrophage cells treated with LPS was investigated. nitric oxide (NO) production, nterleukin$(IL)-1{\alpha}$ IL-6 and IL-10 increased generation of cytokines. mouse macrophage RAW 264.7 cells cell viability changes were no decreas after MTT assay of MF Water extract. The MF water extracts inhibited NO generation caused by LPS in the macrophages over $25{\mu}g/mL$. The MF water extracts increased in the control group the $IL-1{\alpha}$ and IL-6 activation generated by LPS in the macrophages over $50{\mu}g/mL$. Accordingly, it was found that different MF water extract concentrations significantly influenced certain anti-inflammation activities in RAW 264.7 macrophage cells. The results of this study are expected to be highly applicable to health - friendly functional materials. Further studies are needed to confirm the signaling pathways associated with anti-inflammation of macrophages through continuous studies.

상엽 추출물의 항염 효능을 연구하고자 하였다. 상엽을 열수 추출하여 제조한 시료를 대상으로 RAW 264.7 macrophage cells를 24시간 배양하여 세포생존율과 LPS로 유발된 RAW 264.7 macrophage cells의 일산화질소(NO) 생성증가, $IL-1{\alpha}$, IL-6 그리고 IL-10 등의 사이토카인 생성증가에 미치는 영향을 측정하였다. 상엽 추출물의 MTT assay를 수행한 결과 상엽 추출물은 대식세포의 생존율에는 크게 영향을 주지 않았다. LPS로 활성화된 대식세포의 일산화질소(NO)의 생성증가를 $25{\mu}g/mL$ 이상의 농도에서 농도 의존적으로 유의하게 억제시켰다. 또한 LPS로 유발된 대식세포에 염증과 연관된 사이토카인인 $IL-1{\alpha}$, IL-6의 생성증가를 $50{\mu}g/mL$ 이상의 농도에서 농도 의존적으로 유의하게 억제하는 등 항염효과를 가지고 있는 것으로 나타났다. 본 연구 결과는 상엽이 건강 친화적인 기능성 소재로 활용도가 높을 것으로 기대된다. 또한 향후 지속적인 연구를 통한 대식세포를 항염증과 관련된 신호전달경로를 확인하는 추가 실험이 필요하다고 사료된다.

Keywords

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Fig. 1. Effect of MF on cell viability in RAW 264.7 macrophage cells incubated for 24 hrs. Results are the mean ± SD of three independent experiments.

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Fig. 2. Effect of MF on the nitric oxide production in RAW 264.7 macrophage cells were incubated for 24 hrs. treated with LPS; 1 ㎍ /mL. Results are the mean ± SD of three independent experiments.

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Fig. 3. Effect of MF on the IL-1α production in RAW 264.7 macrophage cells were incubated for 24 hrs. treated with LPS; 1 ㎍/mL. Results are the mean ± SD of three independent experiments.

DJTJBT_2018_v16n11_613_f0004.png 이미지

Fig. 4. Effect of MF on the IL-6 production in RAW 264.7 macrophage cells were incubated for 24 hrs. treated with LPS; 1 ㎍/mL. Results are the mean ± SD of three independent experiments.

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Fig. 5. Effect of MF on the IL-10 production in RAW 264.7 macrophage cells were incubated for 24 hrs. treated with LPS; 1 ㎍/mL. Results are the mean ± SD of three independent experiments.

Table 1. Effect of MF on IL-6 Production in Raw 264.7 Cells Treated with LPS

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Table 2. Effect of MF on IL-10 Production in Raw 264.7 Cells Treated with LPS

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