Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Whitening Activity of Abeliophyllum distichum Nakai Leaves According to the Ratio of Prethanol A in the Extracts

  • Jang, Tae-Won (Department of Medicinal Plant Resources, Andong National University) ;
  • Choi, Ji-Soo (Department of Medicinal Plant Science, Jungwon University) ;
  • Kim, Hoi-Ki (Fanipinkorea Co., Ltd) ;
  • Lee, Eun-Ja (Fanipinkorea Co., Ltd) ;
  • Han, Man-Wook (Fanipinkorea Co., Ltd) ;
  • Lee, Ki-Beom (Incheon Business Information Technopark. Biotechnology & Business Center) ;
  • Kim, Do-Wan (Department of Food Science and Industry, Jungwon University) ;
  • Park, Jae-Ho (Department of Pharmaceutical Science, Jungwon University)
  • Received : 2018.10.30
  • Accepted : 2018.12.12
  • Published : 2018.12.31
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Abstract

In this study, we evaluated the whitening activity of prethanol A and water extracts from Abeliophyllum distichum Nakai. The extracts were prepared using 0, 50, 70, and 100% prethanol A at $121^{\circ}C$, 1.2 atm for 15 minutes. To confirm effective extraction, the acteoside content of each extract was analyzed with the HPLC-PDA method. The antioxidant activity was evaluated using DPPH and ABTS scavenging activity assays, and the whitening activity was evaluated based on inhibitory activities on the protein and mRNA expression of tyrosinase, tyrosinase-related protein 1 (TRP-1), tyrosinase-related protein 2 (TRP-2), and microphthalmia-associated transcription factor (MITF) in B16 F10 cells. Each extract showed strong antioxidant and whitening activity. $IC_{50}$ values of antioxidant activity from each extract were in order of 100%, 70%, 50%, and 0%. In addition, whitening activity inhibited the protein and mRNA expression of melanin synthesis factor, following the same pattern as antioxidant activity. In conclusion, water and prethanol A extracts of A. distichum showed effective antioxidant and whitening activity and are thus considered to be valuable materials for whitening cosmetics. The results of this study will also provide basic data for the safe and efficient production of A. distichum as a cosmetic material.

Keywords

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Fig. 1. Acteoside chromatograms from Abeliophyllum distichum Nakai leaves according to the ratio of prethanol A in the extracts by HPLC-PDA.

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Fig. 2. Reducing power of Abeliophyllum distichum Nakai leaves according to the ratio of prethanol A in the extracts.

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Fig. 3. Whitening activities on melanin synthesis related proteins of Abeliophyllum distichum Nakai leaves according to the ratio of prethanol A in the extracts.

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Fig. 4. Whitening activities on melanin synthesis related mRNA levels of Abeliophyllum distichum Nakai leaves according to the ratio of prethanol A in the extracts.

Table 1. DPPH and ABTS radical scavenging activity of Abeliophyllum distichum Nakai leaves according to the ratio of prethanol A in the extracts

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