Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지
DOI QR코드

DOI QR Code

Protective effect of Thymus quinquecostatus extracts UVB-induced matrix metalloproteinase-1 via suppressing MAPKs phosphorylation in human keratinocyte

사람의 각질세포에서 UVB 유도에 따른 MMP-1의 발현 조절과 MAPKs 인산화에 타임 추출물이 미치는 효과

  • Received : 2018.11.01
  • Accepted : 2018.11.22
  • Published : 2018.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Ultraviolet rays are electromagnetic waves with a shorter wavelength than visible light, and ultraviolet rays that pass through the ozone layer are the main cause of skin aging. Chronic exposure of skin tissue to ultraviolet light activates the Mitogen-activated Protein Kinases (MAPKs) signaling pathways in human keratinocytes, resulting in increased production of matrix metalloproteinases (MMPs). In this study, we investigated the herbal extracts from Jeju Island on the anti-aging effect in human keratinocytes (HaCaTs) by ultraviolet stimulation. We examined that herb extract from Jeju Island were decreased in anti-aging activity on measuring the level of MMP-1 gene and protein expression in ultraviolet-induced keratinocytes. As a result, it was confirmed that Thymus quinquecostatus extract (TQE) significantly reduced the expression of MMP-1 in a dose-dependent manner by UV irradiated HaCaTs. According to our data, TQE significantly attenuated UV-induced phosphorylation of the MAPKs signaling elements ERK1/2, JNK1/2 and p38 proteins. These results suggest that the MAPKs pathway may contribute to the inhibitory effect of TQE on UV-induced MMP-1 production in human keratinocytes. Our results suggest that TQE may be a protective agent against skin aging by preventing UV-induced MMP-1 production.

자외선은 가시 광선보다 파장이 짧은 전자기파이며, 오존층을 통과하는 자외선이 피부 노화의 주요 원인이다. 피부 조직을 자외선에 만성적으로 노출 시키면 인간 각질 형성 세포에서 Mitogen-activated Protein Kinases (MAPKs) 신호 전달 경로가 활성화되어 매트릭스 metalloproteinases (MMPs) 생성이 증가한다. 본 연구에서는 제주도의 초본 추출물을 대상으로 자외선 자극을 통해 인간 각질 형성 세포(HaCaTs)의 항 노화 효과에 대해 조사 하였다. 우리는 자외선 유도 된 각질 형성 세포에서 MMP-1 유전자와 단백질 발현 수준을 측정 하였으며 그 결과, Thymus quinquecostatus 추출물(TQE)은 자외선 조사된 HaCaT 세포에서 의해 농도 의존적으로 MMP-1의 발현을 유의하게 감소시키는 것으로 확인되었다. 또한, TQE는 MAPK 신호 전달요소 인 ERK1/2, JNK1/2 및 p38 단백질의 자외선 유도에 따른 인산화를 유의 적으로 감소시켰다. 이러한 결과는 MAPKs 경로가 인간 각질 세포에서 UV 유도 MMP-1 생산에 대한 TQE의 억제 효과에 기여할 수 있음을 시사한다. 우리의 결과는 아마도 인간 각질형성 세포에서 TQE가 자외선 유도된 MMP-1 생산을 억제하는 피부 노화 소재로 활용될 수 있음을 시사한다.

Keywords

References

  1. Jenkins G (2002) Molecular mechanisms of skin ageing. Mech Ageing Dev 123: 801-810 https://doi.org/10.1016/S0047-6374(01)00425-0
  2. Pittayapruek P, Meephansan J, Prapapan O, Komine M, Ohtsuki M (2016) Role of matrix metalloproteinases in photoaging and photocarcinogenesis. Int J Mol Sci 17: E868 https://doi.org/10.3390/ijms17060868
  3. Ho JH, Lee YH, Park JS, Jun WJ, Kim HK, Hong BS, Shin DH, Cho HY (2005) Protective effects of aucubin isolated from eucommia ulmoides against UVBinduced oxidative stress in human skin fibroblasts. Biol Pharm Bull 28: 1244-1248 https://doi.org/10.1248/bpb.28.1244
  4. Parkinson LG, Toro A, Zhao H, Brown K, Tebbutt SJ, Granville DJ (2015) Grnazyme B mediates both direct and indirect cleavage of extracellular matrix in skin after chronic low-dose ultraviolet light irradiation. Aging Cell 14: 67-77 https://doi.org/10.1111/acel.12298
  5. McCawley LJ, Matrisian LM (2001) Matrix metalloproteinases: they are not just for matrix anymore. Curr Opin. Cell Biol 13 534-540 https://doi.org/10.1016/S0955-0674(00)00248-9
  6. Lauer-Fields JL, Juska D, Fields GB (2002) Matrix metalloproteinases and collagen catabolism. Biopolymers 66: 19-32 https://doi.org/10.1002/bip.10201
  7. Visse R, Nagase H (2003) Matrix metalloproteinases and tissue inhibitors of metalloproteinases. Structure, function, and biochemistry. Circ Res 92: 827-839 https://doi.org/10.1161/01.RES.0000070112.80711.3D
  8. Brennan M, Bhatti H, Nerusu KC, Bhagavathula N, Kang S, Fisher GJ (2003) Matrix metalloproteinase-1 is the major collagenolytic enzyme responsible for collagen damage in UV-irradiated human skin. Photochem Photobiol 78: 43-48 https://doi.org/10.1562/0031-8655(2003)078<0043:MMITMC>2.0.CO;2
  9. Reunanen N, Westermarck J, Hakkinen L, Holmstrom TH, Elo I, Eriksson JE (1998) Enhancement of fibroblast collagenase (matrix metalloproteinase-1) gene expression by ceramide is mediated by extracellular signal-regulated and stress-activated protein kinase pathways. J Biol Chem 273: 5137-5145 https://doi.org/10.1074/jbc.273.9.5137
  10. Chang L, Karin M (2001) Mammalian MAP kinase signalling cascades. Nature 410: 37-40 https://doi.org/10.1038/35065000
  11. Khan N, Syed DN, Pal HC, Mukhtar H, Afaq F (2012) Pomegranate fruit extract inhibits UVB-induced inflammation and proliferation by modulating NF-kB and MAPK signaling pathways in mouse skin. Photochem Photobiol 88: 1126-1134 https://doi.org/10.1111/j.1751-1097.2011.01063.x
  12. Shin S, Kim JH (2004) Antifungal activities of essential oil from Thymus quinquecoststus and T. magnus. Planta Med 70: 1090-1092 https://doi.org/10.1055/s-2004-832654
  13. Kim YS, Hwang JW, Kang SH, Kim EH, Jeon YJ, Jeong JH, Kim HR, Moon SH, Jeon BT, Park PJ (2014) Thymol from Thymus quinquecostatus Celak. protects against tert-butyl hydroperoxide-induced oxidative stress in Chang cells. J Nat Med 68: 154-162 https://doi.org/10.1007/s11418-013-0786-8
  14. Pasparakis M, Courtois G, Hafner M, Schmidt-Supprian M, Nenci A, Toksoy A, Krampert M, Goebeler M, Gillitzer R, Israel A, Krieg T, Rajewsky K, Haase I (2002) TNF-mediated inflammatory skin disease in mice with epidermisspecific deletion of IKK2, Nature 417: 861-866 https://doi.org/10.1038/nature00820
  15. Bell S, Degitz K, Quirling M, Jilg N, Page S, Brand K (2003) Involvement of NFkappaB signalling in skin physiology and disease, Cell Signal 15: 1-7 https://doi.org/10.1016/S0898-6568(02)00080-3