Fig. 1. Soft rot symptoms of amaranth caused by Pectobacterium carotovorum subsp. brasiliense on field (A, B), and artificial symptoms by inoculation (C). On third day after infection, infected plants showed soft rot symptoms and control was not affected by infection of 10 mM MgSO4.
Fig. 2. Phylogenetic tree based on 16S rDNA sequences and recombinase A sequences of Pectobacterium strains. DNA sequences from the NCBI database were aligned using ClustalW and phylogenetic trees were constructed using the neighbor-joining method and visualized with MEGA7. Isolated bacteria is GSA1.
References
- Caselato-Sousa, V. M. and Amaya-Farfan, J. 2012. State of knowledge on amaranth grain: A comprehensive review. J. Food Sci. 77: R93-R104. https://doi.org/10.1111/j.1750-3841.2012.02645.x
- Celine, V. A., Girija, V. K., Sreelathakumary, I. and Abdul Vahab, M. 2013. Selection of amaranth genotypes for resistance to Rhizoctonia solani. Int. J. Veget. Sci. 19: 157-163. https://doi.org/10.1080/19315260.2012.691444
- Charkowski, A. O. 2018. The changing face of bacterial soft-rot diseases. Annu. Rev. Phytopathol. 56: 269-288. https://doi.org/10.1146/annurev-phyto-080417-045906
- Dees, M. W., Lysoe, E., Rossmann, S., Perminow, J. and Brurberg, M. B. 2017. Pectobacterium polaris sp. nov., isolated from potato (Solanum tuberosum). Int. J. Syst. Evol. Microbiol. 67: 5222-5229. https://doi.org/10.1099/ijsem.0.002448
- Khayi, S., Cigna, J., Chong, T. M., Quetu-Laurent, A., Chan, K. G., Helias, V. et al. 2016. Transfer of the potato plant isolates of Pectobacterium wasabiae to Pectobacterium parmentieri sp. nov. Int. J. Syst. Evol. Microbiol. 66: 5379-5383. https://doi.org/10.1099/ijsem.0.001524
- Lee, D. H., Kim, J. B., Lim, J. A., Han, S. W. and Heu, S. 2014. Genetic diversity of Pectobacterium carotovorum subsp. brasiliensis isolated in Korea. Plant Pathol. J. 30: 117-124. https://doi.org/10.5423/PPJ.OA.12.2013.0117
- Nabhan, S., De Boer, S. H., Maiss, E. and Wydra, K. 2013. Pectobacterium aroidearum sp. nov., a soft rot pathogen with preference for monocotyledonous plants. Int. J. Syst. Evol. Microbiol. 63: 2520-2525. https://doi.org/10.1099/ijs.0.046011-0
- Pandey, R. M. 2013. Biotechnological advances in amaranths species and their future outlook in crop improvement - A review. Recent Pat. DNA Gene Seq. 7: 179-186.
- Rastogi, A. and Shukla, S. 2013. Amaranth: a new millennium crop of nutraceutical values. Crit. Rev. Food Sci. Nutr. 53: 109-125. https://doi.org/10.1080/10408398.2010.517876
- Tang, Y. and Tsao, R. 2017. Phytochemicals in quinoa and amaranth grains and their antioxidant, anti-inflammatory, and potential health beneficial effects: a review. Mol. Nutr. Food Res. 61: 1600767. https://doi.org/10.1002/mnfr.201600767
- Waleron, M., Misztak, A., Waleron, M., Franczuk, M., Wielgomas, B. and Waleron, K. 2018. Transfer of Pectobacterium carotovorum subsp. carotovorum strains isolated from potatoes grown at high altitudes to Pectobacterium peruviense sp. nov. Syst. Appl. Microbiol. 41: 85-93. https://doi.org/10.1016/j.syapm.2017.11.005