Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Occurrence of Toxigenic Fusarium spp. and Zearalenone in Scabby Rice Grains and Healthy Ones

붉은곰팡이 감염 벼와 건전 벼의 독성 곰팡이와 제랄레논 발생

  • Jang, Ja Yeong (Microbial Safety Team, National Institute of Agricultural Sciences) ;
  • Kim, Sosoo (Microbial Safety Team, National Institute of Agricultural Sciences) ;
  • Jin, Hyun Suk (Microbial Safety Team, National Institute of Agricultural Sciences) ;
  • Baek, Seul Gi (Microbial Safety Team, National Institute of Agricultural Sciences) ;
  • O, Sujin (Department of Anti-aging, Geochang-gun Agricultural Technology Center) ;
  • Kim, Kyutae (Department of Anti-aging, Geochang-gun Agricultural Technology Center) ;
  • Kim, Jeomsoon (Microbial Safety Team, National Institute of Agricultural Sciences) ;
  • Lee, Theresa (Microbial Safety Team, National Institute of Agricultural Sciences)
  • 장자영 (국립농업과학원유해생물팀) ;
  • 김소수 (국립농업과학원유해생물팀) ;
  • 진현숙 (국립농업과학원유해생물팀) ;
  • 백슬기 (국립농업과학원유해생물팀) ;
  • 오수진 (거창군농업기술센터항노화산업과) ;
  • 김규태 (거창군농업기술센터항노화산업과) ;
  • 김점순 (국립농업과학원유해생물팀) ;
  • 이데레사 (국립농업과학원유해생물팀)
  • Received : 2018.09.27
  • Accepted : 2018.10.19
  • Published : 2018.12.31
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Abstract

We analyzed rice sample with scab (collected in 2017) for fungal occurrence and found that frequency of Fusarium spp. was the highest among fungal genera. Within Fusarium, Fusarium asiaticum-nivalenol type dominated as 79%. Among mycotoxins, zearalenone was the highest toxin detected (1117 ng/g), followed by deoxynivalenol (163 ng/g), 15-acetyl deoxynivalenol (155 ng/g), nivalenol (110 ng/g). Analysis of mycotoxin levels in scabby and healthy grains (collected in 2015) revealed that both grain samples were contaminated with zearalenone. However, level difference of zearalenone between the samples was over 5 times as the scabby samples were 1400-2370 ng/g, while healthy ones were 240-430 ng/g. This result indicates that scabby grains should be removed when harvest to reduce zearalenone contamination in rice.

2017년산 붉은곰팡이 감염 벼시료의 곰팡이 발생을 분석한 결과 Fusarium 속의 발생빈도가 가장 높았다. Fusarium 속 중에서는 Fusarium asiaticum-니발레놀 형이 79%로 우점하였다. 곰팡이독소 중에서는 제랄레논이 1117 ng/g으로 가장 많이 검출되었으며 데옥시니발레놀(163 ng/g), 15-아세틸 데옥시니발레놀(155 ng/g), 니발레놀(110 ng/g)의 순으로 높았다. 2015년산 벼시료를 붉은곰팡이 감염벼와 건전벼로 구분한 후 곰팡이독소 수준을 분석한 결과 두 시료는 모두 제랄레논으로 오염되었다. 그러나 이들 시료 간 제랄레논의 수준차이는 5배 이상이었으며 감염벼는 1400-2370 ng/g, 건전벼는 240-430 ng/g이었다. 이 결과는 벼의 제랄레논 독소 저감화를 위해 벼 수확 시 붉은곰팡이 감염종자의 제거가 필요함을 시사한다.

Keywords

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Fig. 1. Rice grains with healthy or scab appearance. (A) sample #2,(B) sample #3.

Table 1. Frequency (%) of fungal genus observed from the rice sample #1

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Table 2. Species composition (%) of FGSC isolates in the rice sam-ple #1

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Table 3. Level of Fusarium toxins in the mixed rice sample #1

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Table 4. Comparison of toxin levels (ng/g) between healthy and scabby rice samples

SMBRCU_2018_v24n4_308_t0004.png 이미지

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