Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Analytical Method for Sodium Polyacrylate in Processed Food Products by Using Size-exclusion Chromatography

Size-exclusion Chromatography를 활용한 가공식품 중 폴리아크릴산나트륨 분석법 확립

  • Jeong, Eun-Jeong (Department of Food Science & Technology, Chonbuk National University) ;
  • Choi, Yoo-Jeong (Department of Food Science & Technology, Chonbuk National University) ;
  • Lee, Gunyoung (Food Additives and Packaging Division, National Institute of Food and Drug Safety Evaluation) ;
  • Yun, Sang Soon (Food Additives and Packaging Division, National Institute of Food and Drug Safety Evaluation) ;
  • Lim, Ho Soo (Food Additives and Packaging Division, National Institute of Food and Drug Safety Evaluation) ;
  • Kim, MeeKyung (Food Additives and Packaging Division, National Institute of Food and Drug Safety Evaluation) ;
  • Kim, Yong-Suk (Department of Food Science & Technology, Chonbuk National University)
  • 정은정 (전북대학교 식품공학과) ;
  • 최유정 (전북대학교 식품공학과) ;
  • 이근영 (식품의약품안전평가원 첨가물포장과) ;
  • 윤상순 (식품의약품안전평가원 첨가물포장과) ;
  • 임호수 (식품의약품안전평가원 첨가물포장과) ;
  • 김미경 (식품의약품안전평가원 첨가물포장과) ;
  • 김용석 (전북대학교 식품공학과)
  • Received : 2018.07.31
  • Accepted : 2018.09.21
  • Published : 2018.12.30
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Abstract

An analytical method of sodium polyacrylate in processed food products was developed and monitored by using size-exclusion chromatography. GF-7M HQ column and UV/VIS detector were selected based on peak shape and linearity. Flow rate, column oven temperature, and mobile phase were selected as 0.6 mL/min, $45^{\circ}C$, and 50 mM sodium phosphate buffer of pH 9.0, respectively. Samples for analysis of sodium polyacrylate were extracted with 50 mM sodium phosphate buffer of pH 7.0 for 3 hr at $20^{\circ}C$ and 150 rpm. Analytical method validation revealed proper selectivity and calibration curve was selected in the range of 50-500 mg/L, and correlation coefficient of calibration curve was more than 0.9985. Limit of detection of sodium polyacrylate was 10.95 mg/kg and limit of quantification was 33.19 mg/kg. Accuracy and coefficient of variation for sodium polyacrylate analysis was 99.6-127.6%, 3.0-8.3% for intra-day and 94.3-121.9%, 1.3-2.6% for inter-day, respectively. Sodium polyacrylate was detected in 40 samples among monitored 125 processed food products. Detected contents were less than 0.2%, limited by the Food Additives Code. Results suggest the established size-exclusion chromatography method could be used to analyze sodium polyacrylate in processed food products.

가공식품에서 폴리아크릴산나트륨에 대한 분석 방법을 size-exclusion chromatography를 사용하여 개발하고 유통 중인 가공식품에 대해 모니터링 하였다. 분석조건에 대하여 GF-7M HQ column과 UV / VIS 검출기를 피크 모양과 선형성을 기준으로 선택하였으며, 유속, column oven 온도 및 이동상은 각각 0.6 mL/min, $45^{\circ}C$ 및 50 mM sodium phosphate buffer (pH 9.0)으로 선정되었다. 시료의 전처리를 위하여 pH 7.0의 50 mM sodium phosphate buffer로 $20^{\circ}C$, 150 rpm에서 3 시간 동안 추출하였다. 확립된 분석법을 검증한 결과 적절한 선택성을 나타내었으며, 검량선은 50~500 mg/L의 범위에서 선정되었고, 이때 검량선의 상관계수($R^2$)는 0.9985로 나타났다. 폴리아크릴산나트륨의 검출한계(LOD)는 10.95 mg/kg으로 정량한계(LOQ)는 33.19 mg/kg의 값을 얻었으며, 정밀도는 intra-day의 경우 3.0~8.3%를, inter day의 경우 1.3~2.6%로 확인되었다. 정확도는 intra-day의 경우 99.6~127.6%를 나타내었으며, interday의 경우 94.3~121.9%로 확인되었다. 유통 중인 가공식품 125품목을 대상으로 폴리아크릴산나트륨의 함유량을 분석한 결과 40품목에서 검출되었으며, 검출량은 식품첨가물공전의 규격인 0.2% 미만으로 검출되었다. 본 연구결과는 size-exclusion chromatography에 의한 분석방법이 가공식품에서 폴리아크릴산나트륨을 분석하는데 적용할 수 있는 적합한 방법이라는 것을 나타내었다.

Keywords

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