Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Toxicokinetics and oral toxicity of Maesil-cheongs with reduced amygdalin levels

아미그달린 저감화 매실청의 독성동태학적 및 경구독성 연구

  • Kim, Hyeon-Jin (Division of Applied Food System, Major of Food Science & Technology, Seoul Women's University) ;
  • Go, Mi-Ran (Division of Applied Food System, Major of Food Science & Technology, Seoul Women's University) ;
  • Yu, Jin (Division of Applied Food System, Major of Food Science & Technology, Seoul Women's University) ;
  • Hwang, Ji-Soo (Division of Applied Food System, Major of Food Science & Technology, Seoul Women's University) ;
  • Choi, Hyun Woo (Department of Food Science and Biotechnology, Kyonggi University) ;
  • Kim, Hyun-Seok (Department of Food Science and Biotechnology, Kyonggi University) ;
  • Choi, Soo-Jin (Division of Applied Food System, Major of Food Science & Technology, Seoul Women's University)
  • 김현진 (서울여자대학교 식품응용시스템학부 식품공학전공) ;
  • 고미란 (서울여자대학교 식품응용시스템학부 식품공학전공) ;
  • 유진 (서울여자대학교 식품응용시스템학부 식품공학전공) ;
  • 황지수 (서울여자대학교 식품응용시스템학부 식품공학전공) ;
  • 최현우 (경기대학교 식품생물공학과) ;
  • 김현석 (경기대학교 식품생물공학과) ;
  • 최수진 (서울여자대학교 식품응용시스템학부 식품공학전공)
  • Received : 2018.10.17
  • Accepted : 2018.10.26
  • Published : 2018.12.31
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Abstract

In this study, the safety aspect of Maesil-cheongs with reduced amygdalin levels was investigated in terms of toxicokinetics and repeated oral toxicity. Plasma or UVC treatment was utilized to obtain Maesil-cheongs with reduced amygdalin levels. The toxicokinetic study demonstrated that the oral absorption of amygdalin decreased remarkably after a single-dose oral administration of both plasma- and UVC-treated Maesil-cheongs. The fourteen-day repeated oral toxicity study revealed that plasma- or UVC-treated Maesil-cheongs did not cause changes in body weight, food intake, water consumption, and absolute and relative organ weights. No significant effects on hematological and serum biochemical parameters were found. Histopathological examination showed no abnormality or toxicological change. These findings suggest that plasma- and UVC-treated Maesil-cheongs have no toxicity potential, and these processes will be useful to obtain products with safe, reduced amygdalin levels.

본 연구에서는 플라즈마 및 UVC 처리에 의해 매실청에 존재하는 아미그달린 저감화 공정을 개발하여 아미그달린 저감화 매실청에 대한 독성을 독성동태학적 및 14일 반복 경구투여에 의해 평가하였다. 독성동태학적 연구 결과, 플라즈마 및 UVC 처리에 의한 아미그달린 저감화 매실청 투여에 의해 아미그달린의 경구 흡수량이 약 3.7, 3.4배 감소하여 저감화 효과를 확인할 수 있었다. 아미그달린 저감화 매실청의 14일 반복 경구독성시험 결과를 종합하면, 몸무게 변화, 식이 및 물 섭취량, 절대장기 및 상대 장기 무게, 혈액학적 및 혈청학적 분석, 조직 병리학적 분석에서 유의미한 독성현상이 관찰되지 않았다. 따라서 본 연구에서 개발 플라즈마 및 UVC 처리에 의한 아미그달린 저감화 매실청의 독성영향은 없는 것으로 보이며, 이러한 공정은 매실청뿐만 아니라 아미그달린 함유 다양한 제품의 저감화 공정에 활용될 수 있을 것이다.

Keywords

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Fig. 1. Schematic diagram for underwater plasma equipment.

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Fig. 2. HPLC chromatograms of amygdalin standard (A), prunasin standard (B), Maesil-cheong (C), plasma-treated Maesil-cheong (D), and UVC-treated Maesil-cheong (E). 1, amygdalin; 2, prunasin.

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Fig. 3. Plasma concentration-time profiles of plasma-treated or UVC-treated Maesil-cheong after a single-dose oral administration to rats.

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Fig. 4. Changes in body weight gain (A), food intake (B), and water consumption (C) after 14-day repeated oral administration of DW as a control, plasma-treated, or UVC-treated Maesil-cheong to rats.

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Fig. 5. Histopathological examination of kidney, liver, lung, and spleen after 14-day repeated oral administration of DW as a control, plasma-treated, or UVC-treated Maesil-cheong to rats. Images magnification at 50×.

Table 1. Toxicokinetic parameters and oral absorption of plasma- or UVC-treated Maesil-cheong after a single-dose oral administration to rats

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Table 2. Absolute organ weights of rats after 14-day repeated oral administration of plasma- or UVC-treated Maesil-cheong

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Table 4. Hematological and coagulation time values in rats after 14-day repeated oral administration of plasma- or UVC-treated Maesil-cheong

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Table 5. Serum biochemical values in rats after 14-day repeated oral administration of plasma- or UVC-treated Maesil-cheong

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Table 3. Organo-somatic indices of rats after 14-day repeated oral administration of plasma- or UVC-treated Maesil-cheong

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Table 6. Summary of histopathological findings of rats after 14-day repeated oral administration of plasma- or UVC-treated Maesil-cheong

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