The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Comparison of Anti-oxidant Activity and Marker Compounds in Eucommiae Cortex Samples from Regional Groups

지역별 두중(杜仲)의 지표성분 및 항산화 활성 비교

  • Yu, Ok Cheol (Department of Oriental Neuropsychiatry, Jaseng Korean Medicine Hospital) ;
  • Choi, Sung Ryul (Department of Oriental Neuropsychiatry, Jaseng Korean Medicine Hospital) ;
  • Joo, Hwan Soo (Department of Oriental Neuropsychiatry, Jaseng Korean Medicine Hospital) ;
  • Han, Chang (Department of Oriental Neuropsychiatry, Jaseng Korean Medicine Hospital) ;
  • Moon, Hye Yeon (Department of Oriental Obsterics and Gynecology, Jaseng Korean Medicine Hospital) ;
  • Jung, Hwa Jin (Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation) ;
  • Jung, Chan Hun (Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation)
  • 유옥철 (자생한방병원 한방신경정신과) ;
  • 최성률 (자생한방병원 한방신경정신과) ;
  • 주환수 (자생한방병원 한방신경정신과) ;
  • 한창 (자생한방병원 한방신경정신과) ;
  • 문혜연 (자생한방병원 한방부인과) ;
  • 정화진 (자생의료재단 척추관절연구소) ;
  • 정찬헌 (자생의료재단 척추관절연구소)
  • Received : 2018.09.17
  • Accepted : 2018.11.25
  • Published : 2018.11.30
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Abstract

Objectives : It is necessary to manage herbal medicines based on effectiveness by comparing the efficacy of herbal medicines by region. In this study, we compared anti-oxidative activity and marker compounds of Eucommiae Cortex by regional groups. Methods : Eucommiae Cortex grown and harvested in Gangjin, Sancheong, Yeongwol, Jangsu, and Jecheon were used. Eucommiae Cortex was extracted in distilled water at $100^{\circ}C$ for 3 hours, and filtered. filtered. Extract samples were freeze-dried at $-80^{\circ}C$. Comparison of anti-oxidant activity in Eucommiae Cortex samples from regional groups was measured in 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) free radical scavenging, and ferric reducing antioxidant power (FRAP) between regional groups of Eucommiae Cortex. In addition, high-performance liquid chromatography (HPLC) analysis was conducted to compare pinoresinol diglucoside concentration by regional groups. Results : HPLC analysis found that pinoresinol diglucoside concentration, widely known as the marker compound of Eucommiae Cortex, was the highest in Gangwon Yeongwol. There was a significant difference in anti-oxidative activity of Eucommiae Cortex between regional groups as discovered in DPPH, ABTS and FRAP assays. DPPH free radical scavenging was the highest in Jeonbuk Jangsu. ABTS free radical scavenging was the highest in Jeonbuk Jangsu and Chungbuck Jecheon. FRAP was the highest in Jeonbuk Jangsu. Conclusions : Although pinoresinol diglucoside concentration was high, anti-oxidative activity was not proportionately high. Pinoresinol diglucoside concentration was the highest in Gangwon Yeongwol. Anti-oxidative activity was the highest in Jeonbuk Jangsu.

Keywords

DHBCBU_2018_v33n6_29_f0001.tif 이미지

Fig. 1. HPLC analysis of pinoresinol diglucoside concentra- tion in Eucommiae Cortex samples from regional groups.

DHBCBU_2018_v33n6_29_f0002.tif 이미지

Fig. 3. Comparison of anti-oxidative activity by ABTS free radical scavenging in Eucommiae Cortex samples from regional groups.

DHBCBU_2018_v33n6_29_f0003.tif 이미지

Fig. 4. Comparison of anti-oxidative activity by ferric reducing antioxidative activity in Eucommiae Cortex samples from regional groups.

DHBCBU_2018_v33n6_29_f0004.tif 이미지

Fig. 2. Comparison of anti-oxidative activity by DPPH free radical scavenging in Eucommiae Cortex samples from regional groups.

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