Asian-Australasian Journal of Animal Sciences

  • 제31권12호
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  • Pages.1946-1955
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  • 2018
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Effect of keratinase on ileal amino acid digestibility in five feedstuffs fed to growing pigs

  • Huang, Chengfei (State Key Laboratory of Animal Nutrition, Ministry of Agriculture Feed Industry Centre, China Agricultural University) ;
  • Ma, Dongli (State Key Laboratory of Animal Nutrition, Ministry of Agriculture Feed Industry Centre, China Agricultural University) ;
  • Zang, Jianjun (State Key Laboratory of Animal Nutrition, Ministry of Agriculture Feed Industry Centre, China Agricultural University) ;
  • Zhang, Bo (Novus International, Inc) ;
  • Sun, Brian (Novus International, Inc) ;
  • Liu, Ling (State Key Laboratory of Animal Nutrition, Ministry of Agriculture Feed Industry Centre, China Agricultural University) ;
  • Zhang, Shuai (State Key Laboratory of Animal Nutrition, Ministry of Agriculture Feed Industry Centre, China Agricultural University)
  • 투고 : 2017.11.07
  • 심사 : 2018.05.07
  • 발행 : 2018.12.01
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초록

Objective: This study was conducted to evaluate the effect of keratinase (KE) on the apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acids (AA) in rice bran, cottonseed meal (CSM), rapeseed meal (RSM), corn distillers dried grains with solubles (DDGS), and peanut meal (PNM). Methods: Twelve crossbred barrows (Duroc${\times}$Landrace${\times}$Yorkshire, $50.5{\pm}1.4kg$ body weight [BW]) fitted with T-cannulas at the terminal ileum were allotted to a $12{\times}6$ Youden Square design with 12 diets and 6 periods. The treatment diets included rice bran, CSM, RSM, corn DDGS, PNM, or corn-soybean meal (cSBM) supplemented with 0.05% KE or not. Diets were given to pigs at a level of 3% BW in two equal meals. The endogenous AA losses were the mean results of three previously experiments determined by a same nitrogen-free diet fed to pigs. Pigs had free access to water during the experiment. Results: The KE supplementation improved (p<0.05) the AID and SID of Met, Thr, Val, Asp, Cys, and Tyr in rice bran. Inclusion of KE increased (p<0.05) the AID and SID of Met and Val in CSM. The KE supplementation decreased (p<0.05) the AID and SID of His in RSM and all measured AA except for Arg, Met, Trp, Val, Gly, and Pro in corn DDGS. There was an increase (p<0.05) in AID and SID of Leu, Ile, Met, Ala, Cys, Ser, and Tyr in PNM supplemented with KE compared with that without KE. Inclusion of KE increased (p<0.05) the AID and SID of crude protein, Leu, Ile, Phe, Thr, Asp, and Ser in cSBM. Conclusion: This study indicated that KE had different effects on ileal AA digestibility of feedstuffs for growing pigs, which can give some usage directions of KE in swine feed containing those detected feedstuffs.

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참고문헌

  1. Williams CM, Richter CS, MacKenzie JMJr, Shih JCH. Isolation, identification and characterization of a feather-degrading bacterium. Appl Environ Microbiol 1990;56:1509-15.
  2. Lin X, Lee CG, Casale ES, Shih JCH. Purification and characterization of a keratinase from a feather-degrading Bacillus licheniformis strain. Appl Environ Microbiol 1992;58:3271-5.
  3. Shih JCH, Wang JJ. Keratinase technology: from feather degradation and feed additive to prion destruction. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources. Wallingford, UK: CABI; 2006. No.042.
  4. Yu RJ, Ragot J, Blank F. Keratinases: hydrolysis of keratinous substrates by three enzymes of Trichophyton mentagrophytes. Experientia 1972;28:1512-3. https://doi.org/10.1007/BF01957886
  5. Lin X, Shih JCH, Swaisgood HE. Hydrolysis of feather keratin by immobilized keratinase. Appl Environ Microbiol 1996;62:4273-5.
  6. Gradisar H, Friedrich J, Krizaj I, Jerala R. Similarities and specificities of fungal keratinolytic proteases: Comparison of keratinases of Paecilomyces marquandii and Doratomyces microsporus to some known proteases. Appl Microbiol Biotechnol 2005;71:3420-6.
  7. Wang D, Zeng Z, Piao XS, et al. Effects of keratinase supplementation of corn-soybean meal based diets on apparent ileal amino acid digestibility in growing pigs and serum amino acids, cytokines, immunoglobulin levels and loin muscle area in nursery pigs. Arch Anim Nutr 2011;65:290-302. https://doi.org/10.1080/1745039X.2011.586140
  8. Odetallah NH, Wang JJ, Garlich JD, Shih JCH. Keratinase in starter diets improves growth of broiler chicks. Poult Sci 2003;82:664-70. https://doi.org/10.1093/ps/82.4.664
  9. Wang JJ, Garlich JD, Shih JCH. Beneficial effects of versazyme, a keratinase feed additive, on body weight, feed conversion, and breast yield of broiler chickens. J Appl Poult Res 2006;15:544-50. https://doi.org/10.1093/japr/15.4.544
  10. Wang H, Guo Y, Shih JCH. Effects of dietary supplementation of keratinase on growth performance, nitrogen retention and intestinal morphology of broiler chickens fed diets with soybean and cottonseed meals. Anim Feed Sci Technol 2008;140:376-84. https://doi.org/10.1016/j.anifeedsci.2007.04.003
  11. Brandon DL, Friedman M. Immunoassays of soy proteins. J Agric Food Chem 2002;50:6635-42. https://doi.org/10.1021/jf020186g
  12. Sun P, Li DF, Li ZJ, Dong B, Wang FL. Effects of glycinin on IgE-mediated increase of mast cell numbers and histamine release in the small intestine. J Nutr Biochem 2008;19:627-33. https://doi.org/10.1016/j.jnutbio.2007.08.007
  13. Zhao Y, Qin G, Sun Z, et al. Disappearance of immunoreactive glycinin and ${\beta}$-conglycinin in the digestive tract of piglets. Arch Anim Nutr 2008;62:322-30. https://doi.org/10.1080/17450390802190318
  14. Wang JJ, Shih J. Fermentation production of keratinase from Bacillus licheniformis PWD-l and a recombinant B. subtillis FDB-29. J Ind Microbiol Biothnol 1999;22:608-16. https://doi.org/10.1038/sj.jim.2900667
  15. Gradisar H, Kern S, Friedrich J. Keratinase of Doratomyces microspores. Appl Microbiol Biotechnol 2000;53:196-200. https://doi.org/10.1007/s002530050008
  16. Stein HH, Shipley CF, Easter RA. Technical note: A technique for inserting a T-cannula into the distal ileum of pregnant sows. J Anim Sci 1998;76:1433-6. https://doi.org/10.2527/1998.7651433x
  17. AOAC. Official methods of analysis: Association of Official Analytical Chemists, 18th edn. Washington DC, USA; AOAC International; 2005.
  18. Thiex NJ, Anderson S, Gildemeister B. Crude fat, diethyl ether extraction, in feed, cereal grain, and forage (Randall/Soxtec/submersion method): Collaborative study. J AOAC Int 2003;86:888-98.
  19. Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci 1991;74:3583-97. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
  20. Li PL, Wu F, Chen Y, et al. Determination of the energy content and amino acid digestibility of double-low rapeseed cakes fed to growing pigs. Anim Feed Sci Technol 2015;210:243-53. https://doi.org/10.1016/j.anifeedsci.2015.10.012
  21. Stein HH, Seve B, Fuller MF, Moughan PJ, de Lange CFM. Invited review: Amino acid bioavailability and digestibility in pig feed ingredients: Terminology and application. J Anim Sci 2007;85:172-80. https://doi.org/10.2527/jas.2005-742
  22. Shi CX, Liu ZY, Shi M, et al. Prediction of digestible and metabolizable energy content of rice bran fed to growing pigs. Asian-Australas J Anim Sci 2015;28:654-61. https://doi.org/10.5713/ajas.14.0507
  23. Li JT, Li DF, Zang JJ, et al. Evaluation of energy digestibility and prediction of digestible and metabolizable energy from chemical composition of different cottonseed meal sources fed to growing pigs. Asian-Australas J Anim Sci 2012;25:1430-8. https://doi.org/10.5713/ajas.2012.12201
  24. Li PL, Wang FL, Wu F, et al. Chemical composition, energy and amino acid digestibility in double-low rapeseed meal fed to growing pigs. J Anim Sci Biotechnol 2015;6:37. https://doi.org/10.1186/s40104-015-0033-0
  25. NRC. Nutrient requirements of swine. 11th ed. Washington, DC, USA: National Academy Press; 2012.
  26. Li P, Li DF, Zhang HY, et al. Determination and prediction of energy values in corn distillers dried grains with solubles sources with varying oil content for growing pigs. J Anim Sci 2015;93:3458-70. https://doi.org/10.2527/jas.2014-8782
  27. Li QY, Piao XS, Liu JD, et al. Determination and prediction of the energy content and amino acid digestibility of peanut meals fed to growing pigs. Arch Anim Nutr 2014;68:196-210. https://doi.org/10.1080/1745039X.2014.910970
  28. Lagos LV, Stein HH. Chemical composition and amino acid digestibility of soybean meal produced in the United States, China, Argentina, Brazil, or India. J Anim Sci 2017;95:1626-36. https://doi.org/10.2527/jas.2017.1440
  29. Böckle B, Muller R. Reduction of disulfide bonds by Streptomyces pactum during growth on chicken feathers. Appl Environ Microbiol 1997;63:790-2.
  30. Ramnani P, Singh R, Gupta R. Keratinolytic potential of Bacillus licheniformis RG1: structural and biochemical mechanism of feather degradation. Can J Microbiol 2005;51:191-6. https://doi.org/10.1139/w04-123
  31. Gupta R, Ramnani P. Microbial keratinases and their prospective applications: an overview. Appl Microbiol Biotechnol 2006;70:21-33. https://doi.org/10.1007/s00253-005-0239-8
  32. Braude R, Fulford RJ, Low AG. Studies on digestion and absorption in the intestines of growing pigs. Measurements of the flow of digesta and pH. Br J Nutr 1976;36:497-510. https://doi.org/10.1079/BJN19760104
  33. Tu GQ, Ye YJ, Zhang B, Guo MJ, Sun TH. Studies on the biomechanism of keratin decomposed by streptomyces. Acta Agric Univ Jiangxiensis 1998;20:1-5.
  34. Kim WK, Patterson PH. Nutritional value of enzyme or sodium hydroxide treated feathers from dead hens. Poult Sci 2000;79:528-34. https://doi.org/10.1093/ps/79.4.528
  35. Lee GG, Ferket PR, Shih JCH. Improvement of feather digestibility by bacterial keratinase as a feed additive. FASEB J 1991;59:1312.
  36. Pahm AA, Pedersen C, Stein HH. Application of the reactive lysine procedure to estimate lysine digestibility in distillers dried grains with solubles fed to growing pigs. J Agric Food Chem 2008;56:9441-6. https://doi.org/10.1021/jf801618g
  37. Kim BG, Kil DY, Zhang Y, Stein HH. Concentrations of analyzed or reactive lysine, but not crude protein, may predict the concentration of digestible lysine in distillers dried grains with solubles fed to pigs. J Anim Sci 2012;90:3798-808. https://doi.org/10.2527/jas.2011-4692

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  1. Comprehensive overview of the quality of plant‐ And animal‐sourced proteins based on the digestible indispensable amino acid score vol.8, pp.10, 2018, https://doi.org/10.1002/fsn3.1809
  2. Microbial enzymes catalyzing keratin degradation: Classification, structure, function vol.44, pp.None, 2018, https://doi.org/10.1016/j.biotechadv.2020.107607