Journal of the Korea Convergence Society (한국융합학회논문지)

Korea Convergence Society (한국융합학회)
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Anti-inflammatory effect of Porphyra yezoensis ethanol extract through the inhibited NF-κB and JNK activation in LPS-PG stimulated HGF-1 cells

사람 치은섬유모세포에서 NF-κB와 JNK 활성 억제를 통한 돌김 에탄올 추출물의 항염증 효과

  • Received : 2018.09.11
  • Accepted : 2018.12.20
  • Published : 2018.12.28
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Abstract

Human gingival fibroblast (HGF) is the main cell type existed in periodontium and produces a variety of inflammatory mediators by external stimuli. In this study, the anti-inflammatory activity of Porphyra yezoensis ethanol extract (PYEE) on LPS-PG lipopolysaccharide from Porphyromonas gingivalis activated HGF-1 cell. Up-regulated iNOS and COX-2 expressions by LPS-PG were significantly attenuated by PYEE treatment in a dose-dependent manner. In addition, activated nuclear factor $(NF)-{\kappa}B$ was also dose-dependently inhibited by PYEE treatment. Among upstream signaling molecules, PYEE treatment inhibited phosphorylation of c-Jun $NH_2$-terminal kinase (JNK) but did not give any effect on other molecules. On the other hand, one of phase II enzymes, NAD(P)H:quinone dehydrogenase (NQO)-1, was analyzed due to its anti-inflammatory activity, which was upregulated by PYEE treatment. Consequently, PYEE could be candidates for the prevention and treatment of periodontal diseases.

사람치은섬유모세포(human gingival fibroblast, HGF)는 치은조직에 존재하는 주요한 세포의 형태 중 하나로 외부 자극에 반응하여 다양한 염증대사물질을 생산한다. 본 연구에서는 돌김에탄올추출물(PYEE)이 Porphyromonas gingivalis로부터 분리한 lipopolysaccharide로 염증이 유도된 HGF-1 cell에서 항염 효과를 보이는지 분석하고자 하였다. LPS-PG에 의해 과발현된 iNOS와 COX-2는 PYEE의 처리에 의해 농도 의존적으로 발현이 감소되었고, nuclear factor $(NF)-{\kappa}B$ 또한 동일한 양상으로 활성이 억제되었다. 신호전달물질 중 c-Jun $NH_2$-terminal kinase (JNK)의 인산화만이 PYEE에 의해 억제되었다. 그리고 항염 작용에 관여하는 것으로 알려진 2상 효소 중 하나인 NAD(P)H:quinone dehydrogenase (NQO)-1도 분석하였고, 이 효소는 PYEE의 처리에 의해 강하게 발현이 유도가 되었다. 결론적으로 돌김에탄올추출물은 치주질환 에방과 치료를 위한 후보물질로 활용 가능할 것으로 사료된다.

Keywords

OHHGBW_2018_v9n12_81_f0001.png 이미지

Fig. 1. PYEE inhibited protein expression levels of iNOS and COX-2 in LPS-PG stimulated HGF-1 cells. Panel A shows protein expression levels of iNOS and COX-2 by PYEE treatment. All signals were normalized to protein levels of actin, an internal control, and expressed as a ratio (Panel B). Data represent the mean±SD of triplicate experiments. Values sharing the same superscript are not significantly different at p<0.05 by Duncan’s multiple range test.

OHHGBW_2018_v9n12_81_f0002.png 이미지

Fig. 2. LPPYSE-EP Gin hisbtiitmeud laptheods pHhoGrFy-la1ti onc eollfs . NFPa-nκeBl iAn PshYoEwEs. Apllh ossipghnoalrsy lawteerde nsotramtuasl izeodf top 6p5ro tebiyn elexvperless seodf aacst in,a arna tioin te(rnPaaln elc onBtr)o. l, Daantda erexppreersimenetn ts. theV alumees an±sShDar ing of thet riplsicaamtee psu

OHHGBW_2018_v9n12_81_f0003.png 이미지

Fig. 3. LPPYSE-EP Gin hsibtiimteudl atpehdo sHphGoFr-yl1a ticoenl lso.f PJaNnKel iAn JsNhoKw sa npdh ops3ph8o rbyyla tiPoYn EEle.v eAlsl l osf igAnaklts, EwReKre, innotremrnaalilz ecdo nttroo l,p raontedi n exlperveeslss edo f asa ctai n,r ataion t(rPipalniecal teB ).e xDpaetrai mreenptrse.s enVta luthees msehaanri±ngS D thoef dsaifmfeer ents upaet rspc

OHHGBW_2018_v9n12_81_f0004.png 이미지

Fig. 4. PYEE induced protein expression level of NQO-1 in LPS-PG stimulated HGF-1 cells. Panel A shows protein expression levels of NQO-1 by PYEE treatment. All signals were normalized to protein levels of actin, an internal control, and expressed as a ratio (Panel B). Data represent the mean±SD of triplicate experiments. Values sharing the same superscript are not significantly different at p<0.05 by Duncan’s multiple range test.

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