Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Development of a pretreatment method for determination of levels of perfluorinated compounds in foods according to fat and protein contents

지방과 단백질 함량에 따른 식품의 과불화화합물 분석을 위한 전처리 방법 확립

  • Bang, Sunah (Department of Food Technology & Service, Eulji University) ;
  • Park, Na-youn (Department of Health, Environment & Safety, Eulji University) ;
  • Hwang, Youngrim (Department of Food Science and Engineering, Ehwa Womans University) ;
  • Kang, Gil Jin (Food Contaminants Division, National Institute of Food and Drug Safety Evalution, Ministry of Food and Drug Safety) ;
  • Kim, Hye-Jeong (Food Contaminants Division, National Institute of Food and Drug Safety Evalution, Ministry of Food and Drug Safety) ;
  • Kang, Young-Woon (Food Contaminants Division, National Institute of Food and Drug Safety Evalution, Ministry of Food and Drug Safety) ;
  • Kho, Younglim (Department of Health, Environment & Safety, Eulji University) ;
  • Kim, Jung Hoan (Department of Food Technology & Service, Eulji University)
  • 방선아 (을지대학교 식품산업외식학과) ;
  • 박나연 (을지대학교 보건환경안전학과) ;
  • 황영림 (이화여자대학교 식품공학과) ;
  • 강길진 (식품의약품안전처 식품의약품안전평가원 오염물질과) ;
  • 김혜정 (식품의약품안전처 식품의약품안전평가원 오염물질과) ;
  • 강영운 (식품의약품안전처 식품의약품안전평가원 오염물질과) ;
  • 고영림 (을지대학교 보건환경안전학과) ;
  • 김정환 (을지대학교 식품산업외식학과)
  • Received : 2017.08.21
  • Accepted : 2017.11.13
  • Published : 2018.02.28
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Abstract

Perfluorinated compounds (PFCs) have recently been recognized as global environmental pollutants. This study was performed to develop an analytical method for determination of levels of PFCs in food by LC-MS/MS. One hundred and nine food products were divided into two groups based on their fat and protein contents (high and low), following which samples containing high fat and protein contents were pooled and subjected to pretreatment consisting of enzymatic degradation and hexane extraction. The limit of detection of 17 PFCs in the samples were in the range of 0.013-0.145 ng/g. The degrees of precision of detection for group 1 (samples with low fat and protein contents) and group 2 (samples with high fat and protein contents) were 0.8-21.1 and 1.7-28.2%, respectively, with an accuracy of 78.8-109.8% for group 1 and 80-114.5% for group 2. This study indicated that pretreatment of high fat and protein foods with enzymatic degradation and hexane extraction would improve the detection of PFCs in food.

본 연구는 국내 유통되는 다양한 식품을 효율적인 방법으로 전처리하고 LC-MS/MS를 이용해 식품 내 PFCs의 분석법을 확립할 목적으로 실시되었다. 109건의 식품을 지방과 단백질의 함량에 따라 두 그룹으로 나누고, 각각의 시료를 분취하여 혼합한 시료를 사용하였다. 지방과 단백질의 함량이 높은 혼합시료는 효소처리와 헥세인 추출을 한 후 ion-pairing방법으로 전처리 후 분석을 실시하였으며, 17 종의 PFCs에 대한 검출한계 값은 0.013-0.145 ng/mL 였고, 두 그룹의 정확도와 정밀도는 각각 Group 1이 78.8-109.8, 0.8-21.1%, Group 2가 80-114.5, 1.7-28.2% 범위를 보여 안정적인 회수율과 정밀도를 보였다. 본 결과로 볼 때 고지방, 고단백질 식품 중 PFCs의 검출에는 효소처리 및 헥세인 처리가 효과적이었으며, 식품 중 지방과 단백질 함량에 따라 전처리 방법을 결정하는 것이 분석의 정확성을 높일 수 있을 것으로 사료된다. 또한 본 연구결과를 통해 추후 국내 유통되는 다양한 식품 내 PFCs의 분석을 위한 전처리 방법으로 적용 가능할 것으로 사료된다.

Keywords

References

  1. He XM, Dai K, Li A, Chen H. Occurrence and assessment of perfluorinated compounds in fish from the Danjiangkou reservoir and Hanjiang river in China. Food Chem. 174: 180-187 (2015) https://doi.org/10.1016/j.foodchem.2014.11.018
  2. Barbarossa A, Gazzotti T, Farabegoli F, Mancini FR, Zironi E, Badiani A, Pagliuca G. Comparison of perfluoroalkyl substances contamination in farmed and wild-caught European sea bass (Dicentrarchus labrax). Food Control. 63: 224-229 (2016) https://doi.org/10.1016/j.foodcont.2015.12.011
  3. Jogsten IE, Nadal M, van Bavel B, Lindstrm G, Domingo JL. Per-and polyfluorinated compounds (PFCs) in house dust and indoor air in Catalonia, Spain: Implications for human exposure. Environ. Int. 39: 172-180 (2012) https://doi.org/10.1016/j.envint.2011.09.004
  4. Wille K, Bussche JV, Noppe H, De Wulf E, Van Caeter P, Janssen CR, Vanhaecke L. A validated analytical method for the determination of perfluorinated compounds in surface-, sea-and sewagewater using liquid chromatography coupled to time-offlight mass spectrometry. J. Chromatogr. A. 1217: 6616-6622 (2010) https://doi.org/10.1016/j.chroma.2010.03.054
  5. Suja F, Pramanik BK, Zain SM. Contamination, bioaccumulation and toxic effects of perfluorinated chemicals (PFCs) in the water environment: a review paper. Water Sci. Technol. 60: 1533-1544 (2009) https://doi.org/10.2166/wst.2009.504
  6. Lau C, Thibodeaux JR, Hanson RG, Narotsky MG, Rogers JM, Lindstrom AB, Strynar MJ. Effects of perfluorooctanoic acid exposure during pregnancy in the mouse. Toxicol. Sci. 90: 510-518 (2006) https://doi.org/10.1093/toxsci/kfj105
  7. Kennedy GL, Butenhoff JL, Olsen GW, O'Connor JC, Seacat AM, Perkins RG, Farrar DG. The toxicology of perfluorooctanoate. Crit. Rev. Toxicol. 34: 351-384 (2004) https://doi.org/10.1080/10408440490464705
  8. Paiano V, Fattore E, Carra A, Generoso C, Fanelli R, Bagnati R. Liquid chromatography-tandem mass spectrometry analysis of perfluorooctane sulfonate and perfluorooctanoic acid in fish fillet samples. J. Anal. Methods Chem. 2012: 719010 (2012)
  9. Lau C, Anitole K, Hodes C, Lai D, Pfahles-Hutchens A, Seed J. Perfluoroalkyl acids: A review of monitoring and toxicological findings. Toxicol. Sci. 99: 366-394 (2007) https://doi.org/10.1093/toxsci/kfm128
  10. Weihe P, Kato K, Calafat AM, Nielsen F, Wanigatunga AA, Needham LL, Grandjean P. Serum concentrations of polyfluoroalkyl compounds in Faroese whale meat consumers. Environ. Sci. Technol. 42: 6291-6295 (2008) https://doi.org/10.1021/es800695m
  11. Quinete N, Wu Q, Zhang T, Yun SH, Moreira I, Kannan K. Specific profiles of perfluorinated compounds in surface and drinking waters and accumulation in mussels, fish, and dolphins from southeastern Brazil. Chemosphere 77: 863-869 (2009) https://doi.org/10.1016/j.chemosphere.2009.07.079
  12. Haug LS, Salihovic S, Jogsten IE, Thomsen C, van Bavel B, Lindstrm G, Becher G. Levels in food and beverages and daily intake of perfluorinated compounds in Norway. Chemosphere 80: 1137-1143 (2010) https://doi.org/10.1016/j.chemosphere.2010.06.023
  13. Tittlemier SA, Pepper K, Seymour C, Moisey J, Bronson R, Cao XL, Dabeka RW. Dietary exposure of Canadians to perfluorinated carboxylates and perfluorooctane sulfonate via consumption of meat, fish, fast foods, and food items prepared in their packaging. J. Agr. Food Chem. 55: 3203-3210 (2007) https://doi.org/10.1021/jf0634045
  14. Kadar H, Veyrand B, Antignac JP, Durand S, Monteau F, Le Bizec B. Comparative study of low-versus high-resolution liquid chromatography-mass spectrometric strategies for measuring perfluorinated contaminants in fish. Food Addit. Contam. A. 28: 1261-1273 (2011) https://doi.org/10.1080/19440049.2011.583283
  15. Saito N, Harada K, Inoue K, Sasaki K, Yoshinaga T, Koizumi A. Perfluorooctanoate and perfluorooctane sulfonate concentrations in surface water in Japan. J. Occup. Health. 46: 49-59 (2004) https://doi.org/10.1539/joh.46.49
  16. Naile JE, Khim JS, Wang T, Chen C, Luo W, Kwon BO, Giesy JP. Perfluorinated compounds in water, sediment, soil and biota from estuarine and coastal areas of Korea. Environ. Pollut. 158: 1237-1244 (2010) https://doi.org/10.1016/j.envpol.2010.01.023
  17. Schecter A, Colacino J, Haffner D, Patel K, Opel M, Ppke O, Birnbaum L. Perfluorinated compounds, polychlorinated biphenyls, and organochlorine pesticide contamination in composite food samples from Dallas, Texas, USA. Environ. Health Persp. 118: 796-802 (2010) https://doi.org/10.1289/ehp.0901347
  18. Liu J, Li J, Zhao Y, Wang Y, Zhang L, Wu Y. The occurrence of perfluorinated alkyl compounds in human milk from different regions of China. Environ. Int. 36: 433-438 (2010) https://doi.org/10.1016/j.envint.2010.03.004
  19. Yamaguchi M, Arisawa K, Uemura H, Katsuura-Kamano S, Takami H, Sawachika F, Hasegawa M. Consumption of seafood, serum liver enzymes, and blood levels of PFOS and PFOA in the Japanese population. J. Occup. Health. 55: 184-194 (2013) https://doi.org/10.1539/joh.12-0264-OA
  20. Kannan K, Choi JW, Iseki N, Senthilkumar K, Kim DH, Masunaga S, Giesy JP. Concentrations of perfluorinated acids in livers of birds from Japan and Korea. Chemosphere 49: 225-231 (2002) https://doi.org/10.1016/S0045-6535(02)00304-1
  21. Maestri L, Negri S, Ferrari M, Ghittori S, Fabris F, Danesino P, Imbriani M. Determination of perfluorooctanoic acid and perfluorooctanesulfonate in human tissues by liquid chromatography/single quadrupole mass spectrometry. Rapid Commun. Mass Sp. 20: 2728-2734 (2006) https://doi.org/10.1002/rcm.2661
  22. Raab U, Albrecht M, Preiss U, Vlkel W, Schwegler U, Fromme H. Organochlorine compounds, nitro musks and perfluorinated substances in breast milk-Results from Bavarian Monitoring of Breast Milk 2007/8. Chemosphere 93: 461-467 (2013) https://doi.org/10.1016/j.chemosphere.2013.06.013
  23. Karrman A, Lindstrom G. Trends, analytical methods and precision in the determination of perfluoroalkyl acids in human milk. TRAC-Trend. Anal. Chem. 46: 118-128 (2013)
  24. Ericson-Jogsten I, Perell G, Llebaria X, Bigas E, Mart-Cid R, Krrman A, Domingo JL. Exposure to perfluorinated compounds in Catalonia, Spain, through consumption of various raw and cooked foodstuffs, including packaged food. Food Chem. Toxicol. 47: 1577-1583 (2009) https://doi.org/10.1016/j.fct.2009.04.004
  25. Ostertag SK, Chan HM, Moisey J, Dabeka R, Tittlemier SA. Historic dietary exposure to perfluorooctane sulfonate, perfluorinated carboxylates, and fluorotelomer unsaturated carboxylates from the consumption of store-bought and restaurant foods for the Canadian population. J. Agr. Food Chem. 57: 8534-8544 (2009) https://doi.org/10.1021/jf9014125
  26. Clarke DB, Bailey VA, Routledge A, Lloyd AS, Hird S, Mortimer DN, Gem M. Dietary intake estimate for perfluorooctanesulphonic acid (PFOS) and other perfluorocompounds (PFCs) in UK retail foods following determination using standard addition LC-MS/MS. Food Addit. Contam. 27: 530-545 (2010) https://doi.org/10.1080/19440040903476590
  27. Ballesteros-Gomez A, Rubio S, van Leeuwe, S. Tetrahydrofuranwater extraction, in-line clean-up and selective liquid chromatography/ tandem mass spectrometry for the quantitation of perfluorinated compounds in food at the low picogram per gram level. J. Chromatogr. A. 1217: 5913-5921 (2010) https://doi.org/10.1016/j.chroma.2010.07.032
  28. Johnson GD, Patil G.P. Cost analysis of composite sampling for classification. Environ. Ecol. Stat. 8: 91-107 (2001). https://doi.org/10.1023/A:1011374330176
  29. Ropers MH, Durand S, Veyrand B, Beaumal V, Marchand P, Anton M, Le Bizec B. Contamination of food by fluorinated surfactants-Distribution in emulsions and impact on the interfacial protein behaviour. Food Hydrocolloid. 23: 1149-1155 (2009) https://doi.org/10.1016/j.foodhyd.2008.10.001
  30. Herzke D, Huber S, Bervoets L, D'Hollander W, Hajslova J, Pulkrabova J, de Voogt P. Perfluorinated alkylated substances in vegetables collected in four European countries; occurrence and human exposure estimations. Environ. Sci. Pollut. R. 20: 7930-7939 (2013) https://doi.org/10.1007/s11356-013-1777-8
  31. Hradkova P, Poustka J, Hlouskova V, Pulkrabova J, Tomaniova M, Hajslova J. Perfluorinated compounds: Occurrence of emerging food contaminants in canned fish and seafood products. Czech J. Food Sci, 28: 333-342 (2010)
  32. Zhao YG, Wan HT, Law AY, Wei X, Huang YQ, Giesy JP, Wong CK. Risk assessment for human consumption of perfluorinated compound-contaminated freshwater and marine fish from Hong Kong and Xiamen. Chemosphere 85: 277-283 (2011) https://doi.org/10.1016/j.chemosphere.2011.06.002
  33. Vassiliadou I, Costopoulou D, Kalogeropoulos N, Karavoltsos S, Sakellari A, Zafeiraki E, Leondiadis L. Levels of perfluorinated compounds in raw and cooked Mediterranean finfish and shellfish. Chemosphere 127: 117-126 (2015) https://doi.org/10.1016/j.chemosphere.2014.12.081