Asian-Australasian Journal of Animal Sciences

  • 제31권1호
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  • Pages.63-70
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  • 2018
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Isolation and characterization of a novel gossypol-degrading bacteria Bacillus subtilis strain Rumen Bacillus Subtilis

  • Zhang, Yunhua (Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University) ;
  • Zhang, Zhengyou (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Dai, Li (Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University) ;
  • Liu, Ying (Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University) ;
  • Cheng, Maoji (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Chen, Lijuan (College of Animal Science and Technology, Anhui Agricultural University)
  • 투고 : 2017.01.10
  • 심사 : 2017.06.19
  • 발행 : 2018.01.01
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초록

Objective: The aim of the study was to isolate gossypol-degrading bacteria and to assess its potential for gossypol degradation. Methods: Rumen liquid was collected from fistulated cows grazing the experimental pasture. Approximately 1 mL of the rumen liquid was spread onto basal medium plates containing 2 g/L gossypol as the only source of carbon and was then cultured at $39^{\circ}C$ to isolate gossypol-degrading bacteria. The isolated colonies were cultured for 6 h and then their size and shape observed by microscope and scanning electron microscope. The 16S rRNA gene of isolated colonies was sequenced and aligned using National Center for Biotechnology Information-Basic Local Alignment Search Tool. The various fermentation conditions, initial pH, incubation temperature, inoculum level and fermentationperiod were analyzed in cottonseed meal (CSM). The crude protein (CP), total gossypol (TG), and free gossypol (FG) were determined in CSM after fermentation with isolated strain at $39^{\circ}C$ for 72 h. Results: Screening results showed that a single bacterial isolate, named Rumen Bacillus Subtilis (RBS), could use gossypol as a carbon source. The bacterium was identified by 16S rDNA sequencing as being 98% homologous to the sequence of Bacillus subtilis strain GH38. The optimum fermentation conditions were found to be 72 h, $39^{\circ}C$, pH 6.5, moisture 50%, inoculum level $10^7cell/g$. In the optimum fermentation conditions, the FG and TG content in fermented CSM decreased 78.86% and 49% relative to the control. The content of CP and the essential amino acids of the fermented CSM increased respectively, compared with the control. Conclusion: The isolation of a gossypol-degrading bacterium from the cow rumen is of great importance for gossypol biodegradation and may be a valuable potential source for gossypol-degradation of CSM.

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  1. Secretome Analysis and Bioprospecting of Lignocellulolytic Fungal Consortium for Valorization of Waste Cottonseed Cake by Hydrolase Production and Simultaneous Gossypol Degradation pp.1877-265X, 2019, https://doi.org/10.1007/s12649-019-00620-1
  2. Impacts of Dietary Protein from Fermented Cottonseed Meal on Lipid Metabolism and Metabolomic Profiling in the Serum of Broilers vol.21, pp.8, 2020, https://doi.org/10.2174/1389203721666200203152643
  3. Isolation and Identification of a Rumen Lactobacillus Bacteria and Its Degradation Potential of Gossypol in Cottonseed Meal during Solid-State Fermentation vol.9, pp.11, 2018, https://doi.org/10.3390/microorganisms9112200
  4. Gossypol detoxification in the rumen and Helicoverpa armigera larvae: A review vol.7, pp.4, 2021, https://doi.org/10.1016/j.aninu.2021.02.007
  5. Enhancing the Quality of Total Mixed Ration Containing Cottonseed or Rapeseed Meal by Optimization of Fermentation Conditions vol.7, pp.4, 2018, https://doi.org/10.3390/fermentation7040234