Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Lipoteichoic Acid Isolated from Staphylococcus aureus Induces Both Epithelial-Mesenchymal Transition and Wound Healing in HaCaT Cells

  • Kim, Seongjae (Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University) ;
  • Kim, Hyeoung-Eun (Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University) ;
  • Kang, Boyeon (Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University) ;
  • Lee, Youn-Woo (Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University) ;
  • Kim, Hangeun (Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University) ;
  • Chung, Dae Kyun (Graduate School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University)
  • Received : 2017.03.30
  • Accepted : 2017.08.02
  • Published : 2017.10.28
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Abstract

Lipoteichoic acid (LTA), a cell wall component of gram-positive bacteria, is recognized by Toll-like receptor 2, expressed on certain mammalian cell surfaces, initiating signaling cascades that include nuclear factor kappa-light-chain-enhancer of activated B cells (NF-${\kappa}B$) and mitogen-activated protein kinase. There are many structural and functional varieties of LTA, which vary according to the different species of gram-positive bacteria that produce them. In this study, we examined whether LTA isolated from Staphylococcus aureus (aLTA) affects the expression of junction proteins in keratinocytes. In HaCaT cells, tight junction-related gene expression was not affected by aLTA, whereas adherens junction-related gene expression was modified. High doses of aLTA induced the phosphorylation of extracellular signal-regulated protein kinases 1 and 2, which in turn induced the epithelial-mesenchymal transition (EMT) of HaCaT cells. When cells were given a low dose of aLTA, however, NF-${\kappa}B$ was activated and the total cell population increased. Taken together, our study suggests that LTA from S. aureus infections in the skin may contribute both to the outbreak of EMT-mediated carcinogenesis and to the genesis of wound healing in a dose-dependent manner.

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References

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