Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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Rabbit submandibular salivary gland replantation

  • Almansoori, Akram A. (Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University) ;
  • Khentii, Namuun (Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University) ;
  • Hei, Wei-Hong (The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University) ;
  • Seo, Nari (Dental Research Institute, Seoul National University) ;
  • Lee, Sung-Ho (Dental Research Institute, Seoul National University) ;
  • Kim, Soung Min (Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University) ;
  • Lee, Jong Ho (Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University)
  • Received : 2017.04.07
  • Accepted : 2017.07.05
  • Published : 2017.10.31
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Abstract

Objectives: To test the feasibility of submandibular salivary gland (SMG) replantation techniques and the survival of the replanted glands. Such a study can provide a rationale for later allotransplantation procedures, along with implementation of conventional and advanced immunosuppression therapy. Materials and Methods: Six SMG replantations were performed in New Zealand white rabbits. One week postoperatively, $^{99m}Tc$ scintigraphy was performed and the uptake ratio and salivary excretion fraction were calculated. Two to four weeks later, submandibular glands were excised, fixed, and stained with H&E for histomorphometric evaluation. Results: Intraoperatively, all glands showed patent blood perfusion except gland 5. Positive tracer uptake and saliva excretion were documented by scintigraphy. On excision, all of the glands except glands 4 and 5 looked viable, with a red color and patent pedicles. Gland 4 was infected and filled with creamy pus, while gland 5 looked pale and necrotic. Histologically, glands 1, 2, 3, and 6 had preserved normal glandular tissue with slight variations from the contralateral normal glands, as their parenchyma was composed of mildly atrophic acini. Conclusion: Four out of six replanted SMGs successfully survived. The glands maintained good viability and function. Such success depends on safe harvesting, short anastomosis time, and strict control of infection.

Keywords

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