Toxicological Research

  • 제33권4호
  • /
  • Pages.305-313
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  • 2017
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  • 1976-8257(pISSN)
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  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
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Comparative In Vitro Biological Toxicity of Four Kinds of Air Pollution Particles

  • Shin, Han-Jae (KT&G Research Institute) ;
  • Cho, Hyun Gi (College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Park, Chang Kyun (College of Agriculture, Life & Environment Sciences, Chungbuk National University) ;
  • Park, Ki Hong (School of Environmental Science and Engineering, GIST) ;
  • Lim, Heung Bin (College of Agriculture, Life & Environment Sciences, Chungbuk National University)
  • 투고 : 2017.05.07
  • 심사 : 2017.07.31
  • 발행 : 2017.10.15
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초록

Accumulating epidemiological evidence indicates that exposure to fine air pollution particles (APPs) is associated with a variety of adverse health effects. However, the exact physiochemical properties and biological toxicities of fine APPs are still not well characterized. We collected four types of fine particle (FP) (diesel exhaust particles [DEPs], natural organic combustion [NOC] ash, synthetic organic combustion [SOC] ash, and yellow sand dust [YSD]) and investigated their physicochemical properties and in vitro biological toxicity. DEPs were almost entirely composed of ultrafine particles (UFPs), while the NOC, SOC, and YSD particles were a mixture of UFPs and FPs. The main elements in the DEPs, NOC ash, SOC ash, and YSD were black carbon, silicon, black carbon, and silicon, respectively. DEPs exhibited dose-dependent mutagenicity even at a low dose in Salmonella typhimurium TA 98 and 100 strains in an Ames test for genotoxicity. However, NOC, SOC, and YSD particles did not show any mutagenicity at high doses. The neutral red uptake assay to test cell viability revealed that DEPs showed dose-dependent potent cytotoxicity even at a low concentration. The toxicity of DEPs was relatively higher than that of NOC, SOC, and YSD particles. Therefore, these results indicate that among the four FPs, DEPs showed the highest in vitro biological toxicity. Additional comprehensive research studies such as chemical analysis and in vivo acute and chronic inhalation toxicity tests are necessary to determine and clarify the effects of this air contaminant on human health.

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참고문헌

  1. Farina, F., Sancini, G., Mantecca, P., Gallinotti, D., Camatini, M. and Palestini, P. (2011) The acute toxic effects of particulate matter in mouse lung are related to size and season of collection. Toxicol. Lett., 202, 209-217. https://doi.org/10.1016/j.toxlet.2011.01.031
  2. Sancini, G., Farina, F., Battaglia, C., Cifola, I., Mangano, E., Mantecca, P., Camatini, M. and Palestini, P. (2014) Health risk assessment for air pollutants: alterations in lung and cardiac gene expression in mice exposed to Milano winter fine particulate matter (PM 2.5). PLoS ONE, 9, e109685. https://doi.org/10.1371/journal.pone.0109685
  3. Jassal, M.S. (2015) Pediatric asthma and ambient pollutant levels in industrializing nations. Int. Health, 7, 7-15. https://doi.org/10.1093/inthealth/ihu081
  4. Adams, K., Greenbaum, D.S., Shaikh, R., van Erp, A.M. and Russel, A.G. (2015) Particulate matter components, sources, and health: Systematic approaches to testing effects. J. Air Waste Manag. Assoc., 65, 544-558. https://doi.org/10.1080/10962247.2014.1001884
  5. Luanpitpong, S., Chen, M., Knuckles, T., Wen, S., Luo, J., Ellis, E., Hendryx, M. and Rojanasakul, Y. (2014) Appalachian mountaintop mining particulate matter induces neoplastic transformation of human bronchial epithelial cells and promotes tumor formation. Environ. Sci. Technol., 48, 12912-12919. https://doi.org/10.1021/es504263u
  6. Gong, J., Zhu, T., Kipen, H., Rich, D.Q., Huang, W., Lin, W.T., Hu, M. and Zhang, J.J. (2015) Urinary polycyclic aromatic hydrocarbon metabolites as biomarkers of exposure to traffic-emitted pollutants. Environ. Int., 85, 104-110. https://doi.org/10.1016/j.envint.2015.09.003
  7. Dumax-Vorzet, A.F., Tate, M., Walmsley, R., Elder, R.H. and Povey, A.C. (2015) Cytotoxicity and genotoxicity of urban particulate matter in mammalian cells. Mutagenesis, 30, 621-633. https://doi.org/10.1093/mutage/gev025
  8. Prange, J.A., Gaus, C., Weber, R., Papke, O. and Muller, J.F. (2003) Assessing forest fire as a potential PCDD/F source in Queensland, Australia. Environ. Sci. Technol., 37, 4325-4329. https://doi.org/10.1021/es0343454
  9. Simon, E., Choi, S.D. and Park, M.K. (2016) Understanding the fate of polycyclic aromatic hydrocarbons at a forest fire site using a conceptual model based on field monitoring. J. Hazard. Mater., 317, 632-639. https://doi.org/10.1016/j.jhazmat.2016.06.030
  10. Noma, Y., Yamamoto, T., Giraud, R. and Sakai, S. (2006) Behavior of PCNs, PCDDs, PCDFs, and dioxin-like PCBs in the thermal destruction of wastes containing PCNs. Chemosphere, 62, 1183-1195. https://doi.org/10.1016/j.chemosphere.2005.07.039
  11. Yamada, P., Hatta, T., Du, M., Wakimizu, K., Han, J., Maki, T. and Isoda, H. (2012) Inflammatory and degranulation effect of yellow sand on RBL-2H3 cells in relation to chemical and biological constituents. Ecotoxicol. Environ. Saf., 84, 9-17. https://doi.org/10.1016/j.ecoenv.2012.05.021
  12. Hayakawa, K. (2016) Environmental behaviors and toxicities of polycyclic aromatic hydrocarbons and nitropolycyclic aromatic hydrocarbons. Chem. Pharm. Bull., 64, 83-94. https://doi.org/10.1248/cpb.c15-00801
  13. Nadal, M., Marques, M., Mari, M. and Domingo, J.L. (2015) Climate change and environmental concentrations of POPs: A review. Environ. Res., 143, 177-185. https://doi.org/10.1016/j.envres.2015.10.012
  14. Sagai, M., Saito, H., Ichinose, T., Kodama, M. and Mori, Y. (1993) Biological effects of diesel exhaust particles. I. In vitro production of superoxide and in vivo toxicity in mouse. Free Radic. Biol. Med., 14, 37-47. https://doi.org/10.1016/0891-5849(93)90507-Q
  15. Maron, D.M. and Ames, B.N. (1983) Revised methods for the Salmonella mutagenicity test. Mutat. Res., 113, 173-215 https://doi.org/10.1016/0165-1161(83)90010-9
  16. Borenfreund, E. and Puerner, J.A. (1985) Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol. Lett., 24, 119-124. https://doi.org/10.1016/0378-4274(85)90046-3
  17. Surawski, N.C., Miljevic, B., Avoko, G.A., Elbaqir, S., Stevanovic, S., Fairfull-Smith, K.E., Bottle, S.E. and Ristovski, Z.D. (2011) Physicochemical characterization of particulate emissions from a compression ignition engine: the influence of biodiesel feedstock. Environ. Sci. Technol., 45, 10337-10343. https://doi.org/10.1021/es2018797
  18. Faustini, A., Alessandrini, E.R., Pey, J., Perez, N., Samoli, E., Querol, X., Cadum, E., Perrino, C., Ostro, B., Ranzi, A., Sunyer, J., Stafoggia, M. and Forastiere, F. (2015) Short-term effects of particulate matter on mortality during forest fires in Southern Europe: results of the MED-PARTICLES project. Occup. Environ. Med., 72, 323-329. https://doi.org/10.1136/oemed-2014-102459
  19. Naeher, L.P., Brauer, M., Lipsett, M., Zelikoff, J.T., Simpson, C.D., Koeniq, J.Q. and Smith, K.R. (2007) Woodsmoke health effects: a review. Inhal. Toxicol., 19, 67-106.
  20. Yang, H.H., Luo, S.W., Lee, K.T., Wu, J.Y., Chang, C.W. and Chu, P.F. (2016) Fine particulate speciation profile and emission factor of municipal solid waste incinerator established by dilution sampling method. J. Air Waste Manag. Assoc., 66, 807-814. https://doi.org/10.1080/10962247.2016.1184195
  21. Fujimori, T., Tanino, Y. and Takaoka, M. (2014) Coexistence of Cu, Fe, Pb, and Zn oxides and chlorides as a determinant of chlorinated aromatics generation in municipal solid waste incinerator fly ash. Environ. Sci. Technol., 48, 85-92. https://doi.org/10.1021/es403585h
  22. Ma, Q., Liu, Y., Liu, C., Ma, J. and He, H. (2012) A case study of Asian dust storm particles: chemical composition, reactivity to SO2 and hygroscopic properties. J. Environ. Sci. (China), 24, 62-71. https://doi.org/10.1016/S1001-0742(11)60729-8
  23. Noh, Y., Muller, D., Shin, S.K., Shin, D. and Kim, Y.J. (2016) Vertically-resolved profiles of mass concentrations and particle backscatte coefficients of Asian dust plumes derived from lidar observations of silicon dioxide. Chemosphere, 143, 24-31. https://doi.org/10.1016/j.chemosphere.2015.03.037
  24. Jalava, P.I., Raimo, O.S., Kati, N., Arto, S.P., Mikko, S.H., Jarkko, T., Anna, F., Risto, H., Jorma, J. and Maija-Riitta, H. (2010) Effect of combustion condition on cytotoxic and inflammatory activity of residential wood combustion particles. Atmos. Environ., 44, 1691-1698. https://doi.org/10.1016/j.atmosenv.2009.12.034
  25. Hashimoto, A.H., Amanuma, K., Hiyoshi, K., Sugawara, Y., Goto, S., Yanagisawa, R., Takano, H., Masumura, K., Nohmi, T. and Aoki, Y. (2007) Mutations in the lungs of gpt delta transgenic mice following inhalation of diesel exhaust. Environ. Mol. Mutagen., 48, 682-693. https://doi.org/10.1002/em.20335
  26. Rivedal, E., Myhre, O., Sanner, T. and Eide, I. (2003) Supplemental role of the Ames mutation assay and gap junction intercellular communication in studies of possible carcinogenic compounds from diesel exhaust particles. Arch. Toxicol., 77, 533-542. https://doi.org/10.1007/s00204-003-0483-6
  27. Gaffney, J.S. and Nancy, A.M. (2009) The impacts of combustion emissions on air quality and climate - From coal to biofuels and beyond. Atmos. Environ., 43, 23-36. https://doi.org/10.1016/j.atmosenv.2008.09.016
  28. Manzo, N.D., Slade, R., Richards, J.H., McGee, J.K., Martin, L.D. and Dye, J.A. (2010) Susceptibility of inflamed alveolar and airway epithelial cells to injury induced by diesel exhaust particles of varying organic carbon content. J. Toxicol. Environ. Health Part A, 73, 565-580. https://doi.org/10.1080/15287390903566625

피인용 문헌

  1. Comparison of Mutagenic Activities of Various Ultra-Fine Particles vol.34, pp.2, 2018, https://doi.org/10.5487/TR.2018.34.2.163