Clean Technology (청정기술)

The Korean Society of Clean Technology (한국청정기술학회)
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Manufacturing of Monodisperse Pectin Hydrogel Microfibers Using Partial Gelation in Microfluidic Devices

미세유체 장치에서 부분젤화법을 이용한 단분산성 펙틴 하이드로젤 미세섬유의 제조

  • Jin, Si Hyung (Department of Chemical Engineering, Chungnam National University) ;
  • Kim, Chaeyeon (Department of Energy Science and Technology, Graduate School of Energy Science and Technology, Chungnam National University) ;
  • Lee, Byungjin (Department of Chemical Engineering, Chungnam National University) ;
  • Shim, Kyu-Rak (Department of Chemical Engineering, Chungnam National University) ;
  • Kim, Dong Young (Department of Chemical Engineering, Chungnam National University) ;
  • Lee, Chang-Soo (Department of Chemical Engineering, Chungnam National University)
  • 진시형 (충남대학교 공과대학 화학공학과) ;
  • 김채연 (충남대학교 에너지과학기술대학원 에너지과학기술학과) ;
  • 이병진 (충남대학교 공과대학 화학공학과) ;
  • 심규락 (충남대학교 공과대학 화학공학과) ;
  • 김동영 (충남대학교 공과대학 화학공학과) ;
  • 이창수 (충남대학교 공과대학 화학공학과)
  • Received : 2017.04.18
  • Accepted : 2017.04.27
  • Published : 2017.09.30
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Abstract

This study introduces a method to easily fabricate highly monodisperse pectin hydrogel microfibers in a microfluidic device by using partial gelation. The hydrodynamic parameters between the pectin aqueous solution and the calcium ions containing oil solution are precisely controlled to form a stable elongation flow of the pectin aqueous solution, and partial gelation of the pectin aqueous solution is performed by the chelating of the calcium ions at the interface between the two phases. The partially gelled pectin aqueous solution is phase-separated from the oil solution in an aqueous calcium chloride solution outside the microfluidic device and is completely gelled to produce monodisperse pectin hydrogel microfibers. The thickness of the pectin hydrogel microfiber is controlled in a reproducible manner by controlling the volumetric flow rate of the initially injected pectin aqueous solution. The pectin hydrogel microfibers were 200 to 500 micrometers in diameter and had a coefficient of variation below 5% under all thickness conditions, indicating that the pectin hydrogel microfibers produced by partial gelation are highly monodisperse. In addition, biomaterials can be immobilized to the pectin hydrogel microfibers produced by a single process, demonstrating the possibility that our pectin hydrogel microfiber can be used as carriers for biomaterials or tissue engineering.

본 연구는 미세유체 장치에서 매우 균일한 펙틴 하이드로젤 미세섬유를 부분젤화법을 통해 손쉽게 제조하는 방법을 소개한다. 펙틴 수용액과 이와 섞이지 않는 칼슘이 분산된 오일용액 사이의 수력학적 변수들을 조절하여 펙틴 수용액의 흐름을 안정적으로 늘어진 유동을 형성하고 두 상의 계면에서 칼슘 이온의 킬레이트화 반응으로 펙틴 수용액을 부분젤화 시킨다. 부분젤화된 펙틴 수용액은 미세유체 장치 외부의 염화칼슘 수용액에서 오일 용액과 상분리 되고 완전히 젤화되어 미세섬유로 제조된다. 펙틴 하이드로젤 미세섬유의 굵기는 초기 주입되는 펙틴 수용액의 부피유속을 조절함으로써 재현성 있게 제어된다. 제조된 펙틴 하이드로젤 미세섬유의 직경은 200에서 500 마이크로미터 범위이며 모든 두께 조건에서 5% 이하의 변동계수를 가짐으로써 매우 균일함을 증명하였다. 또한 펙틴 하이드로젤 미세섬유에 생체물질을 단일공정으로 고정화 함으로써 생체물질 담지체나 조직공학의 지지체로써 사용될 수 있는 가능성을 보여준다.

Keywords

References

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