한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제32권3호
  • /
  • Pages.243-248
  • /
  • 2017
  • /
  • 2508-755X(pISSN)
  • /
  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
권호 표지
DOI QR코드

DOI QR Code

TREK통로 차단제의 한우 정자 생존성 및 운동성 억제 효과

Inhibitory Effect of TREK Channel Blockers on Sperm Viability and Motility of Korean Native Bull

  • 강다원 (경상대학교 의과대학 생리학교실) ;
  • 김은진 (경상대학교 의과대학 생리학교실) ;
  • 한재희 (경상대학교 의과대학 생리학교실)
  • Kang, Dawon (Department of Physiology, College of Medicine, Gyeongsang National University) ;
  • Kim, Eun-Jin (Department of Physiology, College of Medicine, Gyeongsang National University) ;
  • Han, Jaehee (Department of Physiology, College of Medicine, Gyeongsang National University)
  • 투고 : 2017.09.20
  • 심사 : 2017.09.25
  • 발행 : 2017.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Antioxidants have been added to cryoprotectant or in vitro culture medium for sperm to reduce the detrimental damage, such as reactive oxygen species. However, curcumin, an antioxidant, shows dual effect on the viability and progressive motility of bovine sperm exposed to hydrogen peroxide. Low concentration of curcumin increases sperm viability and progressive motility, whereas high concentration of curcumin reduces them. This study was performed to identify whether TREK-1 channel is related to low sperm viability and motility induced by high concentration of curcumin. Curcumin reduced TREK-1 channel activity in a dose-dependent manner. TREK-1 channel was expressed in sperm obtained from Korean native bull. Treatment with TREK-1 channel blockers, such as curcumin, fluoxetine, $GdCl_3$, and spadin, significantly reduced sperm viability and motility (p < 0.05). However, TREK-1 channel activators showed different effect; linoleic acid showed an increase in sperm viability and motility, and wogonin did not affect them. These results show that TREK-1 channel is involved in the regulation of sperm viability and motility. In particular, high concentration of curcumin might reduce sperm viability and progressive motility of Korean native bull through blockage of TREK-1 channel.

키워드

참고문헌

  1. Almeida FC, Silva SV, Souza HM, Gomes WA, Lima Filho JA, Wicke AA, Batista AM and Guerra MM. 2017. Effects of glycerol, equilibration time and antioxidants on post-thaw functional integrity of bovine spermatozoa directly obtained from epididymis. Andrologia 49:e12623. https://doi.org/10.1111/and.12623
  2. Amidi F, Pazhohan A, Shabani Nashtaei M, Khodarahmian M and Nekoonam S. 2016. The role of antioxidants in sperm freezing: a review. Cell and Tissue Bank 17:745-756. https://doi.org/10.1007/s10561-016-9566-5
  3. Banday MN, Lone FA, Rasool F, Rashid M and Shikari A. 2017. Use of antioxidants reduce lipid peroxidation and improve quality of crossbred ram sperm during its cryopreservation. Cryobiology 74:25-30. https://doi.org/10.1016/j.cryobiol.2016.12.008
  4. Chen LG, Hung LY, Tsai KW, Pan YS, Tsai YD, Li YZ and Liu YW. 2008. Wogonin, a bioactive flavonoid in herbal tea, inhibits inflammatory cyclooxygenase-2 gene expression in human lung epithelial cancer cells. Mol. Nutr. Food Res. 52:1349-1357. https://doi.org/10.1002/mnfr.200700329
  5. Danthi S, Enyeart JA and Enyeart JJ. 2003. Modulation of Native TREK-1 and Kv1.4 $K^{+}$ Channels by Polyunsaturated Fatty Acids and Lysophospholipids. J. Membrane Biol. 195:147-164. https://doi.org/10.1007/s00232-003-0616-0
  6. Enyeart JA, Liu H and Enyeart JJ. 2008. Curcumin inhibits bTREK-1 $K^{+}$ channels and stimulates cortisol secretion from adrenocortical cells. Biochem. Biophys. Res. Commun. 370:623-628. https://doi.org/10.1016/j.bbrc.2008.04.001
  7. Hur CG, Choe C, Kim GT, Cho SK, Park JY, Hong SG, Han J and Kang D. 2009. Expression and localization of two-pore domain $K^{+}$ channels in bovine germ cells. Reproduction 137:237-244. https://doi.org/10.1530/REP-08-0035
  8. Kim D. 2003. Fatty acid-sensitive two-pore domain $K^{+}$ channels. Trends Pharmacol. Sci. 24:648-54. https://doi.org/10.1016/j.tips.2003.10.008
  9. Kim EJ, Kang D and Han J. 2011. Baicalein and wogonin are activators of rat TREK-2 two-pore domain $K^{+}$ channel. Acta Physiologica 202:185-192. https://doi.org/10.1111/j.1748-1716.2011.02263.x
  10. Kumar A and Singh A. 2015. A review on mitochondrial restorative mechanism of antioxidants in Alzheimer's disease and other neurological conditions. Front. Pharmacol. 6:206.
  11. Kumar G, Mittal S, Sak K and Tuli HS. 2016. Molecular mechanisms underlying chemopreventive potential of curcumin: Current challenges and future perspectives. Life Sci. 148:313-328. https://doi.org/10.1016/j.lfs.2016.02.022
  12. Lim W, Jeong M, Bazer FW and Song G. 2016. Curcumin Suppresses Proliferation and Migration and Induces Apoptosis on Human Placental Choriocarcinoma Cells via ERK1/2 and SAPK/JNK MAPK Signaling Pathways. Biol. Reprod. 95:83. https://doi.org/10.1095/biolreprod.116.141630
  13. Liu X, Tian S, Liu M, Jian L and Zhao L. 2016. Wogonin inhibits the proliferation and invasion, and induces the apoptosis of HepG2 and Bel7402 HCC cells through $NF-{\kappa}B$/Bcl-2, EGFR and EGFR downstream ERK/AKT signaling. Int. J. Mol. Med. 38:1250-1256. https://doi.org/10.3892/ijmm.2016.2700
  14. Lu L, Zhang G, Song C, Wang X, Qian W, Wang Z, Liu Y, Gong S and Zhou S. 2017. Arachidonic acid has protective effects on oxygen-glucose deprived astrocytes mediated through enhancement of potassium channel TREK-1 activity. Neurosci. Lett. 636:241-247. https://doi.org/10.1016/j.neulet.2016.11.034
  15. Park BH, Lim JE, Jeon HG, Seo SI, Lee HM, Choi HY, Jeon SS and Jeong BC. 2016. Curcumin potentiates antitumor activity of cisplatin in bladder cancer cell lines via ROS-mediated activation of ERK1/2. Oncotarget 7:63870-63886.
  16. Sapanidou V, Taitzoglou I, Tsakmakidis Ι, Kourtzelis I, Fletouris D, Theodoridis A, Zervos I and Tsantarliotou M. 2015. Antioxidant effect of crocin on bovine sperm quality and in vitro fertilization. Theriogenology 84:1273-1282. https://doi.org/10.1016/j.theriogenology.2015.07.005
  17. Tejada S, Manayi A, Daglia M, Nabavi SF, Sureda A, Hajheydari Z, Gortzi O. Pazoki-Toroudi H and Nabavi SM. 2016. Wound Healing Effect of Curcumin: A Review Curr. Pharm. Biotechnol. 17:1002-1007. https://doi.org/10.2174/1389201017666160721123109
  18. Tvrda E, Tusimova E, Kovacik A, Paal D, Greifova H, Abdramanov A and Lukac N. 2016. Curcumin has protective and antioxidant properties on bull spermatozoa subjected to induced oxidative stress. Anim. Reprod. Sci. 172:10-20. https://doi.org/10.1016/j.anireprosci.2016.06.008
  19. Wu X, Zhang H, Salmani JM, Fu R and Chen B. 2016. Advances of wogonin, an extract from Scutellaria baicalensis, for the treatment of multiple tumors. Onco. Targets Ther. 9:2935-2943.
  20. 김양미, 김경아. 2011. TREK-1 채널에 대한 플라보노이드의 효과. Journal of the Korea Academia-Industrial cooperation Society 12:2660-2667. https://doi.org/10.5762/KAIS.2011.12.6.2660
  21. 전예진, 김대영. 2013. 미니돼지 정자의 동결보존에 미치는 Curcumin의 항산화 효과. J. Chitin. Chitosan. 18:190-197
  22. 화정석, 김은진, 류지현, Adrian S. Siregar, 박창윤, 최창용, 강다원. 2016. 산화스트레스에 노출된 정자의 생존성 및 운동성에 있어서 커큐민의 이중효과. J. Emb. Trans. 31:299-305 https://doi.org/10.12750/JET.2016.31.3.299