DOI QR코드

DOI QR Code

Molecular weight-associated cellular response to silk fibroin fragments demonstrated in MG63 cells

  • Jo, You-Young (Sericultural and Apicultural Materials Division, National Institute of Agricultural Science) ;
  • Kweon, HaeYong (Sericultural and Apicultural Materials Division, National Institute of Agricultural Science) ;
  • Kim, Seong-Gon (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University)
  • Received : 2017.06.23
  • Accepted : 2017.07.11
  • Published : 2017.09.30

Abstract

In this study, changes in gene expression after administration of silk fibroin fragments ($size{\approx}30kDa$) were evaluated in MG63 cells using a cDNA microarray assay. In addition, the level of alkaline phosphatase (ALP) activity and cellular proliferation in the group administered moderately sized silk fibroin fragments ($size{\approx}30kDa$) (MSF) were compared to those in the group administered smaller silk fibroin fragments (size < 1 kDa) (SSF). The results of the cDNA microarray assay show increased expression of genes that are related to the cell cycle and inflammation. ALP, bone morphogenetic protein-7, bone morphogenetic protein receptor type IA, and runt-related transcription factor 2 exhibited significantly lower expression compared to control cells (fold ratio < 0.5). Relative ALP activity of the $100{\mu}g/mL$ MSF group was significantly lower than that of the SSF group (P < 0.05). Thus, the MSF group showed increased expression of genes associated with cellular proliferation and inflammation but decreased expression of genes associated with osteogenesis.

Keywords

References

  1. Bataiosu M, Taisescu CI, Pisoschi CG, Pascu EI, Tuculina MJ, Daguci L, et al (2015) Effects of therapy with two combinations of antibiotics on the imbalance of MMP-2${\div}$TIMP-2 in chronic periodontitis. Rom J Morphol Embryol 56, 77-83.
  2. Brown J, Lu CL, Coburn J, Kaplan DL (2015) Impact of silk biomaterial structure on proteolysis. Acta Biomater 11, 212-221. https://doi.org/10.1016/j.actbio.2014.09.013
  3. Cao Y, Wang B (2009) Biodegradation of silk biomaterials. Int J Mol Sci 10, 1514-1524. https://doi.org/10.3390/ijms10041514
  4. Hadziabdic N, Kurtovic-Kozaric A, Pojskic N, Sulejmanagic N, Todorovic L (2016) Gene-expression analysis of matrix metalloproteinases 1 and 2 and their tissue inhibitors in chronic periapical inflammatory lesions. J Oral Pathol Med 45, 224-230. https://doi.org/10.1111/jop.12347
  5. Jo YY, Kweon H, Kim DW, Kim MK, Kim SG, Kim JY, et al (2017) Accelerated biodegradation of silk sutures through matrix metalloproteinase activation by incorporating 4-hexylresorcinol. Sci Rep 7, 42441. https://doi.org/10.1038/srep42441
  6. Kim JY, Choi JY, Jeong JH, Jang ES, Kim AS, Kim SG, et al (2010) Low molecular weight silk fibroin increases alkaline phosphatase and type I collagen expression in MG63 cells. BMB Rep 43, 52-56. https://doi.org/10.5483/BMBRep.2010.43.1.052
  7. Kweon H, Kim SG, Choi JY (2014) Inhibition of foreign body giant cell formation by 4-hexylresorcinol through suppression of diacylglycerol kinase delta gene expression. Biomaterials 35, 8576-8584. https://doi.org/10.1016/j.biomaterials.2014.06.050
  8. Lee EH, Kim JY, Kweon HY, Jo YY, Min SK, Park YW, et al (2010) A combination graft of low-molecular-weight silk fibroin with Choukroun platelet-rich fibrin for rabbit calvarial defect. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 109, e33-e38.
  9. Lee SW, Um IC, Kim SG, Cha MS (2015) Evaluation of bone formation and membrane degradation in guided bone regeneration using a 4-hexylresorcinol-incorporated silk fabric membrane. Maxillofac Plast Reconstr Surg 37, 32. https://doi.org/10.1186/s40902-015-0034-0
  10. Little DM, Haynes LD, Alam T, Geraghty JG, Sollinger HW, Hullett DA (1999) Does transforming growth factor beta 1 play a role in the pathogenesis of chronic allograft rejection? Transpl Int 12, 393-401.
  11. Liu B, Song YW, Jin L, Wang ZJ, Pu DY, Lin SQ, et al (2015) Silk structure and degradation. Colloids Surf B Biointerfaces 131, 122-128. https://doi.org/10.1016/j.colsurfb.2015.04.040
  12. Morris AH, Stamer D, Kyriakides T (2017) The host response to naturally-derived extracellular matrix biomaterials. Semin Immunol In press (doi:10.1016/j.smim.2017.01.002).
  13. Panilaitis B, Altman GH, Chen J, Jin HJ, Karageorgiou V, Kaplan DL (2003) Macrophage responses to silk. Biomaterials 24, 3079-3085. https://doi.org/10.1016/S0142-9612(03)00158-3
  14. Park AR, Park YH, Kim HJ, Kim MK, Kim SG, Kweon H, et al (2015) Tri-layered silk fibroin and poly-${\epsilon}$-caprolactone small diameter vascular grafts tested in vitro and in vivo. Macromol Res 23, 924-936. https://doi.org/10.1007/s13233-015-3126-x
  15. Seok H, Kim MK, Kim SG, Kweon H (2014) Comparison of silkworm-cocoon-derived silk membranes of two different thicknesses for guided bone regeneration. J Craniofac Surg 25, 2066-2069. https://doi.org/10.1097/SCS.0000000000001151
  16. Thurber AE, Omenetto FG, Kaplan DL (2015) In vivo bioresponse to silk proteins. Biomaterials 71, 145-157. https://doi.org/10.1016/j.biomaterials.2015.08.039
  17. Wong L, Hutson PR, Bushman W (2014) Prostatic inflammation induces fibrosis in a mouse model of chronic bacterial infection. PLoS One 9, e100770. https://doi.org/10.1371/journal.pone.0100770
  18. Yoo CK, Jeon JY, Kim YJ, Kim SG, Hwang KG (2016) Cell attachment and proliferation of osteoblast-like MG63 cells on silk fibroin membrane for guided bone regeneration. Maxillofac Plast Reconstr Surg 38, 17. https://doi.org/10.1186/s40902-016-0062-4