식물분류학회지 (Korean Journal of Plant Taxonomy)

  • 제47권3호
  • /
  • Pages.222-235
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  • 2017
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  • 1225-8318(pISSN)
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  • 2466-1546(eISSN)
한국식물분류학회 (The Korean Society of Plant Taxonomists)
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나도국수나무족(장미과) 잎 표피 미세형태학적 형질의 계통학적 유용성

The systematic implications of leaf micromorphological characteristics in the tribe Neillieae (Spiraeoideae, Rosaceae)

  • 송준호 (경희대학교 이과대학 생물학과) ;
  • 홍석표 (경희대학교 이과대학 생물학과)
  • Song, Jun-Ho (Laboratory of Plant Systematics, Department of Biology, Kyung Hee University) ;
  • Hong, Suk-Pyo (Laboratory of Plant Systematics, Department of Biology, Kyung Hee University)
  • 투고 : 2017.08.02
  • 심사 : 2017.09.18
  • 발행 : 2017.09.30
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초록

나도국수나무족에 속하는 Neillia속(나도국수나무속) 4종 4변종, Physocarpus속(산국수나무속) 5종, Stephanandra속(국수나무속) 2종을 포함, 총 3속 15분류군의 잎 표피 미세형태학적 형질을 주사전자현미경(scanning electron microscope)을 이용하여 관찰 및 기재하였으며, 형질의 분류학적, 계통학적 유용성을 검토하였다. Neillia속, Stephanandra속에서 기공복합체는 모두 배축면에만 존재하는 이면기공엽(hypostomatic type)이 나타났고, Physocarpus속에서는 P. monogynus, P. opulifolius에서만 양면기공엽(amphistomatic type)이 나타났다. 공변세포의 크기는 $12.02-34.39{\times}10.76-27.13{\mu}m$로 속과 종마다 다소 차이를 보이는데, N. thyrsiflora가 평균 $13.98{\times}12.43(L{\times}W){\mu}m$로 가장 작은 공변세포를 갖는 것으로 나타났으며, N. gracilis가 평균 $26.82{\times}20.67(L{\times}W){\mu}m$로 가장 크게 나타났다. 기공복합체의 형태는 N. affinis에서만 평행형(paracytic)이 나타났고, 대부분 불규칙형(anomocytic)이 확인되었다. 표피세포는 섬국수나무(Physocarpus insularis)에서만 배축면에서 유두상(papillate)이 관찰되어 구별되었으며, N. affinis, S. tanakae의 향축면에서 판형(platelets)의 표피상납질(epicuticular wax)이 나타나 구별되었다. 한편, 연구된 분류군에서 모용은 크게 단세포단모(unicellular non-glandular trichome), 2-5개 가지상 모용(two- to five-armed trichome), 성상모(stellate), 선모(glandular trichome), 총 4종류로 확인되었다. 특히, 섬국수나무를 제외한 Physocarpus속 모든 분류군에서 성상모가 나타나 구별되었다. 끝으로, 잎 표피 미세형태학적 형질 중 기공복합체의 형태, 유두상표피세포의 유무, 성상모의 유무 형질이 분류학적 및 계통학적으로 유용성이 있음을 밝혔고, 본 연구 결과, Stephanandra속의 Neillia속으로의 통합, 섬국수나무(Physocarpus insularis)의 Spiraea속(조팝나무속)으로의 전속을 주장한 이전의 분자계통학적 및 화분학적 연구 결과를 강력하게 지지하였다.

A comparative study of the leaf epidermal micromorphology in the tribe Neillieae (Neillia: 4 species, 4 varieties; Physocarpus: 5 species; Stephanandra: 2 species) was carried out using scanning electron microscopy in order to evaluate the taxonomic and systematic implications of these characteristics. The leaves of the genera Neillia and Stephanandra were hypostomatic, whereas those of P. monogynus, P. opulifolius were amphistomatic. The range of the size of the stomata is $12.02-34.39{\times}10.76-27.13{\mu}m$; the smallest was found in N. thyrsiflora (average $13.98{\times}12.43{\mu}m$; $L{\times}W$), while the largest was measured in N. gracilis (average $26.82{\times}20.67{\mu}m$; $L{\times}W$). Paracytic stomata complexes are only found in N. affinis, and the anomocytic type was most commonly found. The papillate epidermal cell type was only observed on the abaxial surfaces of P. insularis. Platelet epicuticular waxes were found on the adaxial surfaces of N. affinis and S. tanakae. Four types (unicellular non-glandular, two- to five-armed, stellate, and glandular) of trichomes were found on the leaves. Stellates were observed in all species of Physocarpus except for P. insularis. Consequently, leaf epidermal micromorphological characteristics (e.g., the presence of papillate epidermal cells and stellate, and stomata complexes) may have high taxonomic and systematic value in Neillieae. Our results strongly support previous molecular phylogenetic and palynological hypotheses that Stephanandra and Neillia are a single genus and that Physocarpus insularis should be considered as a member of Spiraea.

키워드

참고문헌

  1. Adeniji, K. A. and J. O. Ariwaodo. 2012. Comparative foliar epidermal studies of genus Pericopsis (Papilionaceae) in Nigeria. Phytologia Balcanica 18: 37-41.
  2. Agrawal, A. A., M. Fishbein, R. Jetter, J. P. Salminen, J. B. Goldstein, A. E. Freitag and J. P. Sparks. 2009. Phylogenetic ecology of leaf surface traits in the milkweeds (Asclepias spp.): chemistry, ecophysiology, and insect behavior. New Phytologist 183: 848-867. https://doi.org/10.1111/j.1469-8137.2009.02897.x
  3. Anil Kumar, V. S. and K. Murugan. 2013. Taxonomic significance of foliar micromorphology and their systematic relevance in the genus Solanum (Solanaceae). In Prospects in Bioscience: Addressing the Issues. Sabu, A. and A. Augustine (eds.), Springer, New Delhi. Pp. 343-349.
  4. Barthlott, W. 1981. Epidermal and seed surface characters of plants: systematic applicability and some evolutionary aspects. Nordic Journal of Botany 1: 345-355. https://doi.org/10.1111/j.1756-1051.1981.tb00704.x
  5. Barthlott, W., C. Neinhuis, D. Cutler, F. Ditsch, I. Meusel, I. Theisen and H. Wilhelmi. 1998. Classification and terminology of plant epicuticular waxes. Botanical Journal of the Linnean Society 126: 237-260. https://doi.org/10.1111/j.1095-8339.1998.tb02529.x
  6. Barthlott, W., I. Theisen, T. Borsch and C. Neinhuis. 2003. Epicuticular waxes and vascular plant systematics: integrating micromorphological and chemical data. In Deep Morphology: Toward a Renaissance of Morphology in Plant Systematics. Stuessy, T. F., V. Mayer and E. Horandl (eds.), ARG Gantner Verlag, Ruggell. Pp. 189-206.
  7. Bentham, G. and J. D. Hooker. 1865. Genera Plantarum, Vol. 1. William Pamplin, London, 516 pp.
  8. Cronquist, A. 1981. An Integrated System of Classification of Flowering Plants. Columbia University Press, New York, 1262 pp.
  9. Dahlgren, R. M. T. 1980. A revised system of classification of the angiosperms. Botanical Journal of the Linnean Society 80: 91-124. https://doi.org/10.1111/j.1095-8339.1980.tb01661.x
  10. De Sa-Haiad, B., A. C. C. Serpa-Ribeiro, C. N. Barbosa, D. Pizzini, D. D. O. Leal, L. de Senna-Valle and L. D. R. De Santiago-Fernandes. 2009. Leaf structure of species from three closely related genera from tribe Crotoneae Dumort. (Euphorbiaceae s.s., Malpighiales). Plant Systematics and Evolution 283: 179-202. https://doi.org/10.1007/s00606-009-0229-x
  11. Deng, M., A. Hipp, Y.-G. Song, Q.-S. Li, A. Coombes and A. Cotton. 2014. Leaf epidermal features of Quercus subgenus Cyclobalanopsis (Fagaceae) and their systematic significance. Botanical Journal of the Linnean Society 176: 224-259. https://doi.org/10.1111/boj.12207
  12. Ensikat, H. J., M. Boese, W. Mader, W. Barthlott and K. Koch. 2006. Crystallinity of plant epicuticular waxes: electron and X-ray diffraction studies. Chemistry and Physics of Lipids 144: 45-59. https://doi.org/10.1016/j.chemphyslip.2006.06.016
  13. Eriksen, B. and B. A. Yurstev. 1999. Hair types in Potentilla sect. Niveae (Rosaceae) and related taxa, terminology and systematic distribution. Norske Videnskaps-Akademi Matematisk.-Naturvidenskapelig Klasse 38: 201-221.
  14. Esau, K. 1977. Anatomy of Seed Plants. 2nd ed. John Wiley and Sons, New York, 576 pp.
  15. Faghir, M. B., F. Attar, A. Farazmand, B. Ertter and B. Eriksen. 2010. Leaf indumentum types in Potentilla (Rosaceae) and related genera in Iran. Acta Societatis Botanicorum Poloniae 79: 139-145.
  16. Faghir, M. B., K. K. Chaichi and R. S. Shahvon. 2014. Foliar epidermis micromorphology of the genus Alchemilla (Rosaceae) in Iran. Phytologia Balcanica 20: 215-225.
  17. Ganeva, T. and K. Uzunova. 2010. Comparative leaf epidermis study in species of genus Malus Mill. (Rosaceae). Botanica Serbica 34: 45-49.
  18. Greene, E. L. 1889. The North American Neilliae. Pittonia 2: 25-31.
  19. Heo, K. I., S. R. Lee, M. H. Yoo, S. T. Lee, Y. Kwon, S. Y. Lim, S. H. Kim and S. C. Kim. 2013. The taxonomic implication of trichome and epicuticular waxes in tribe Potentilleae (Rosaceae) in Korea. Korean Journal of Plant Taxonomy 43: 106-117. (in Korean) https://doi.org/10.11110/kjpt.2013.43.2.106
  20. Husain, S. Z., P. D. Marin, C. Silic, M. Qaiser, and B. Petcovic. 1990. A micromorphological study of some representative genera in the tribe Saturejeae (Lamiaceae). Botanical Journal of the Linnean Society 103: 59-80. https://doi.org/10.1111/j.1095-8339.1990.tb00174.x
  21. Jones, M. E. 1893. Contributions to western botany. Zoe 4: 38-45.
  22. Kalkman, C. 2004. Rosaceae. In The Families and Genera of Vascular Plants, Vol. 6. Flowering Plants - Dicotyledons: Celastrales, Oxalidales, Rosales, Cornales, Ericales. Kubitzki, K. (ed.), Springer-Verlag, Berlin. Pp. 343-386.
  23. Kim, C. H., T. J. Kim and B.-Y. Sun. 2000. Taxonomic identities of some endemic Korean vascular plants. Korean Journal of Plant Taxonomy 30: 355-361. (in Korean) https://doi.org/10.11110/kjpt.2000.30.4.355
  24. Kolodziejek, J. 2008. Hair types in Polish selected taxa of Potentilla subsect. Collinae (Rosaceae). Acta Societatis Botanicorum Poloniae 77: 217-224.
  25. Kuntze, O. 1891. Revisio Generum Plantarum, Parts 1. Arthur Felix, Leipzig, 374 pp.
  26. Liu, Q., D. X. Zhang and P. M. Peterson. 2010. Lemma micromorphological characters in the Chloridoideae (Poaceae) optimized on a molecular phylogeny. South African Journal of Botany 76: 196-209. https://doi.org/10.1016/j.sajb.2009.10.006
  27. Ma, Q.-W., C.-S. Li, F.-L. Li and S. V. Vickulin. 2004. Epidermal structures and stomatal parameters of Chinese endemic Glyptostrobus pensilis (Taxodiaceae). Botanical Journal of the Linnean Society 146: 153-162. https://doi.org/10.1111/j.1095-8339.2004.00326.x
  28. Maximowicz, C. J. 1879. Adnotationes de Spiraeaceis. Trudy Imperatorskago S.-Peterburgskago Botanicheskago Sada 6: 105-261.
  29. Moise, J. A., S. Han, L. Gudynaite-Savitch, D. A. Johnson and B. L. A. Miki. 2005. Seed coats: structure, development, composition, and biotechnology. In Vitro Cellular & Developmental Biology-Plant 41: 620-644. https://doi.org/10.1079/IVP2005686
  30. Moon, H.-K., S.-P. Hong, E. Smets and S. Huysmans. 2009. Phylogenetic significance of leaf micromorphology and anatomy in the tribe Mentheae (Nepetoideae: Lamiaceae). Botanical Journal of the Linnean Society 160: 211-231. https://doi.org/10.1111/j.1095-8339.2009.00979.x
  31. Moon, H.-K., E. Smets and S. Huysmans. 2010. Phylogeny of tribe Mentheae (Lamiaceae): the story of molecules and micromorphological characters. Taxon 59: 1065-1076.
  32. Morgan, D. R., D. E. Soltis and K. R. Robertson. 1994. Systematic and evolutionary implications of rbcL sequence variation in Rosaceae. American Journal of Botany 81: 890-903. https://doi.org/10.1002/j.1537-2197.1994.tb15570.x
  33. Oh, S.-H. 2006. Neillia includes Stephanandra (Rosaceae). Novon 16: 91-95. https://doi.org/10.3417/1055-3177(2006)16[91:NISR]2.0.CO;2
  34. Oh, S.-H. 2015. Taxonomy of tribe Neillieae (Rosaceae): Physocarpus. Korean Journal of Plant Taxonomy 45: 332-352. https://doi.org/10.11110/kjpt.2015.45.4.332
  35. Oh, S.-H. 2016. Taxonomy of tribe Neillieae (Rosaceae): Neillia. Korean Journal of Plant Taxonomy 46: 13-32. https://doi.org/10.11110/kjpt.2016.46.1.13
  36. Oh, S.-H. and D. Potter. 2003. Phylogenetic utility of the second intron of LEAFY in Neillia and Stephanandra (Rosaceae) and implications for the origin of Stephanandra. Molecular Phylogenetics and Evolution 29: 203-215. https://doi.org/10.1016/S1055-7903(03)00093-9
  37. Oh, S.-H. and D. Potter. 2005. Molecular phylogenetic systematics and biogeography of tribe Neillieae (Rosaceae) using DNA sequences of cpDNA, rDNA, and LEAFY. American Journal of Botany 92: 179-192. https://doi.org/10.3732/ajb.92.1.179
  38. Oh, S.-H., L. Chen, S.-H. Kim, Y.-D. Kim and H. Shin. 2010. Phylogenetic relationship of Physocarpus insularis (Rosaceae) endemic on Ulleung Island: implications for conservation biology. Journal of Plant Biology 53: 94-105. https://doi.org/10.1007/s12374-009-9093-z
  39. Oliveira, A. F. M., S. T. Meirelles and A. Salatino. 2003. Epicucticular waxes from caatinga and cerrado species and their efficiency against water loss. Anais da Academia Brasileira de Ciencias 75: 431-439. https://doi.org/10.1590/S0001-37652003000400003
  40. Potter, D., F. Gao, P. E. Bortiri, S.-H. Oh and S. Baggett. 2002. Phylogenetic relationships in Rosaceae inferred from chloroplast matK and trnL-trnF nucleotide sequence data. Plant Systematics and Evolution 231: 77-89. https://doi.org/10.1007/s006060200012
  41. Potter, D., T. Eriksson, R. C. Evans, S. Oh, J. E. E. Smedmark, D. R. Morgan, M. Kerr, K. R. Robertson, M. Arsenault, T. A. Dickinson and C. S. Campbell. 2007. Phylogeny and classification of Rosaceae. Plant Systematics and Evolution 266: 5-43. https://doi.org/10.1007/s00606-007-0539-9
  42. Rehder, A. 1940. The Manual of Cultivated Trees and Shrubs Hardy in North America Exclusive of the Subtropical and Warmer Temperate Regions. 2nd ed. Macmillan, New York, 996 pp.
  43. Robertson, K. R. 1974. The genera of Rosaceae in the southeastern United States. Journal of the Arnold Arboretum 55: 303-332.
  44. Rotondi, A., F. Rossi, C. Asunis and C. Cesaraccio. 2003. Leaf xeromorphic adaptations of some plants of a coastal Mediterranean macchia ecosystem. Journal of Mediterranean Ecology 4: 25-35.
  45. Schulze-Menz, G. K. 1964. Rosaceae. In Engler's Syllabus der Pflanzenfamilien II. 12th ed. Melchior H. (ed.), Gebruder Borntraeger, Berlin. Pp. 209-218.
  46. Shin, H., Y.-D. Kim and S.-H. Oh. 2011. A new combination in Spiraea (Rosaceae) from Ulleung Island, Korea. Novon 21: 373-374. https://doi.org/10.3417/2009126
  47. Song, J.-H. 2016. Systematic studies in Spiraeoideae (Rosaceae). Ph.D. dissertation, Kyung Hee University, Seoul, Korea, 335 pp.
  48. Song, J.-H. and S.-P. Hong. 2016. Taxonomic significance of the leaf micromorphology in the tribe Sorbarieae (Spiraeoideae: Rosaceae). Korean Journal of Plant Taxonomy 46: 199-212. (in Korean) https://doi.org/10.11110/kjpt.2016.46.2.199
  49. Song, J.-H., H.-K. Moon, M.-K. Oak and S.-P. Hong. 2017a. Phylogenetic evaluation of pollen and orbicule morphology in the Rosaceae tribe Neillieae (subfamily Amygdaloideae). Botanical Journal of the Linnean Society 183: 439-453. https://doi.org/10.1093/botlinnean/bow019
  50. Song, J.-H., M.-K. Oak, H.-S. Roh and S.-P. Hong. 2017b. Morphology of pollen and orbicules in the tribe Spiraeeae (Rosaceae) and its systematic implications. Grana 56: 351-367. https://doi.org/10.1080/00173134.2016.1274334
  51. Stace, C. A. 1984. The taxonomic importance of the leaf surface. In Current Concepts in Plant Taxonomy. Heywood, V. H. and D. M. Moore (eds.), Academic Press, London. Pp. 67-93.
  52. Taia, W. K. 2004. Leaf characters within tribe Trifolieae (family Leguminosae). Pakistan Journal of Biological Sciences 7: 1463-1472. https://doi.org/10.3923/pjbs.2004.1463.1472
  53. Takhtajan, A. 1997. Diversity and Classification of Flowering Plants. Columbia University Press, New York, 620 pp.
  54. Theobald, W. L., J. L. Krahulik and R. C. Rollins. 1979. Trichome description and classification. In Anatomy of the Dicotyledons. 2nd ed. Vol. I. Metcalfe, C. R. and L. Chalk (eds.), Clarendon Press, Oxford. Pp. 40-53.
  55. Thiers, B. 2017. (Continuously updated) Index Herbariorum: a global directory of public herbaria and associated staff. New York Botanical Garden's Virtual Herbarium. Retrieved Sep. 1, 2016, Available from http://sweetgum.nybg.org/ih/.
  56. Vidal, J. 1963. Le genre Neillia (Rosaceae). Adansonia 3: 142-166.
  57. Wilkinson, H. P. 1979. The plant surface (mainly leaf). In Anatomy of the Dicotyledons. 2nd ed. Vol. I. Metcalfe, C. R. and L. Chalk (eds.), Clarendon Press, Oxford. Pp. 97-165.
  58. Yang, Z.-R. and Q. Lin. 2005. Comparative morphology of the leaf epidermis in Schisandra (Schisandraceae). Botanical Journal of the Linnean Society 148: 39-56. https://doi.org/10.1111/j.1095-8339.2005.00396.x
  59. Zoric, L., L. Merkulov, J. Lukovic, P. Boza and D. Polic. 2009. Leaf epidermal characteristics of Trifolium L. species from Serbia and Montenegro. Flora 204: 198-209. https://doi.org/10.1016/j.flora.2008.02.002

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