Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Evaluation of Lipid Peroxidation Inhibition and Nitrogen Oxide Scavenging Activity from Peel of Gardenia jasminoides Ellis Fructus Extracted by Various Solvents

치자(Gardenia jasminoides Ellis fructus) 껍질 용매 별 추출물의 지질과산화 저해 및 질소산화물 소거능

  • Jin, Dong-Hyeok (Department of Food Science and Technology, Pusan National University) ;
  • Oh, Da-Young (Department of Food Science and Technology, Pusan National University) ;
  • Chung, Hun-Sik (Department of Food Science and Technology, Pusan National University) ;
  • Lee, Young-Guen (Department of Food Science and Technology, Pusan National University) ;
  • Seong, Jong-Hwan (Department of Food Science and Technology, Pusan National University) ;
  • Kim, Han-Soo (Department of Food Science and Technology, Pusan National University)
  • Received : 2017.05.02
  • Accepted : 2017.06.21
  • Published : 2017.06.30
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Abstract

The aim of this study was to investigate the bioactivity and antioxidant activity of peel from Gardenia jasminoides Ellis fructus (GJE). We were separated into GJE peel. After that, we determined anthocyanin. GJE peel were extracted by 70% methanol, distilled water (DW) and ethyl acetate (EA) three solvents. To investigate by the solvent extract of total phenol content and value as a functional food ingredient of GJE peel through nitrogen oxide scavenging activity, antioxidant activity, reducing power and lipid peroxidation inhibition were performed. Solvent extract bioactivity of increasing concentrations (0.2, 0.4, 0.6 mg/mL) were significantly increased (p<0.05). GJE peel extracts showed lower activity than positive control (ascorbic acid, BHA, trolox). The total phenol contents of GJE peel extracts were highest in EA extract. However, the order of total phenol content of the solvent in the GJE peel and the results of analysis of various physiological activities were inconsistent. Considering the extraction yield and various physiological activities, it is expected to be effective when extracted from 70% methanol and DW extract. The results suggest that GJE peel is highly expected to be useful as a functional foods and natural antioxidant.

치자 껍질의 70% methanol, ethyl acetate (EA) 및 distilled water (DW)의 용매 별 추출물의 total phenol 함량 및 질소 산화물 소거능, 환원력, ${\beta}$-carotene 탈색을 이용한 항산화력 및 지질과 산화 저해능 측정을 통하여 치자의 기능성 식품 소재로서의 가치를 검토한 결과, anthocyanin 함량은 $3.519{\pm}0.635mg/100g\;DW$로 나타났으며, 용매 별 추출 수율은 DW (39.87%), 70% methanol (36.26%), EA (2.88%)로 관찰되었다. 추출 용매 별 항산화 활성은 농도(0.2, 0.4, 0.6 mg/mL)가 증가할수록 유의적으로 증가하였으며 control로 사용된 ascorbic acid, BHA, trolox 보다는 낮은 활성이 확인되었다. 치자 껍질의 total phenol 함량은 EA, 70% methanol, DW 추출물 순으로 EA 추출물에서 $26.59{\pm}0.20CAE$ (caffeic acid equivalents) mg/g으로 가장 높았으며, Nitric oxide (NO) radical 소거능에서는 70% methanol (70.32~76.15%), DW (52.66~59.31%), EA (34.65~46.98%) 추출물 순으로 나타났다. Nitrite ($NO_2$) 소거능은 70% methanol (34.57~39.33%), DW (32.53~38.47%), EA (27.59~32.62%) 순으로 관찰되었다. ${\beta}$-carotene 탈색 저해능은 DW (41.55~50.97%), 70% methanol (23.37~44.80%), EA (13.37~25.24%) 순으로 동정되었다. Reducing power (optical density)는 70% methanol (0.044~0.127), DW (0.033~0.099), EA (0.026~0.097) 순으로 확인되었다. 지질과산화 저해능은 껍질 추출물 중 70% methanol (56.51~76.21%), EA (54.59~63.34%), DW (47.92~61.11%) 순으로 관찰되었다(p<0.05). 이에, 치자 껍질은 기능성 식품 및 천연 항산화제로서의 가치가 매우 높을 것으로 판단된다.

Keywords

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