Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Antioxidant and anti-inflammatory activity of extracts from kohlrabi (Brassica Oleracea var. Gonglodes)

콜라비 추출물의 항산화 및 항염 효능

  • Yi, Mi-Ran (Biotechnology Regional Innovation Center, Jeju National University) ;
  • Kang, Chang-Hee (Biotechnology Regional Innovation Center, Jeju National University) ;
  • Bu, Hee-Jung (Biotechnology Regional Innovation Center, Jeju National University)
  • 이미란 (제주대학교 생명과학기술혁신센터) ;
  • 강창희 (제주대학교 생명과학기술혁신센터) ;
  • 부희정 (제주대학교 생명과학기술혁신센터)
  • Received : 2017.05.24
  • Accepted : 2017.06.14
  • Published : 2017.06.30
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Abstract

This study was designed to examine the in vitro antioxidant and anti-inflammatory effects of kohlrabi (Brassica oleracea var. gonglodes) extract. Kohlrabi was extracted using 70% ethanol and then fractionated sequentially with n-hexane, ethyl acetate and butanol. Antioxidative ability was evaluated by bioassays using total polyphenol contents and ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid diammonium salt) radical scavenging activity. Ethyl acetate fraction of kohlrabi was best on total polyphenol contents ($27.33{\pm}0.26mg\;GAE/g$) and ABTS radical scavenging effects ($IC_{50}\;172.9{\pm}1.6{\mu}g/mL$).For the anti-inflammatory activity in RAW 264.7 cells, the EtOAc fraction showed the highest inflammatory effect. Dose response studies were performed to determine the inhibitory effect of EtOAc fraction of kohlrabi on pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The EtOAc fraction of kohlrabi inhibited the NO and $PGE_2$ production and the protein level of iNOS and COX-2, and protein expression of pro-inflammatory cytokines (TNF-${\alpha}$, IL-6 and IL-$1{\beta}$), in a dose-dependent manner. These results suggest that kohlrabi has considerable potential as a ingredient with antioxidative and anti-inflammatory effects.

본연구는 콜라비 추출물의 항산화 및 항염 효능을 조사하기 위하여 수행하였다. 콜라비는 70% 에탄올을 이용하여 조추출한 후 헥산, 에틸아세테이트, 부탄올을 이용하여 용매 극성에 따라 순차적으로 분획하였다. 항산화 활성은 총폴리페놀 함량 측정과 ABTS 라디칼 소거활성을 측정하여 평가하였다. 에틸아세테이트 분획물이 총폴리페놀 함량($27.33{\pm}0.26mg\;GAE/g$)과 ABTS 라디칼 소거활성($IC_{50}\;172.9{\pm}1.6{\mu}g/mL$)이 가장 높게 측정되었다. 항염 활성은 RAW 264.7 세포를 이용하였으며, 에틸아세테이트 분획물이 가장 높은 항염 활성을 보였다. 에틸아세테이트 분획물의 LPS로 유도된 RAW 264.7 세포에서 전염증성 매개인자들에 대한 저해효과를 농도별로 측정하여 확인하였다. 콜라비 에틸아세테이트 분획물은 NO, $PGE_2$ 생성과 iNOS와 COX-2 및 TNF-${\alpha}$, IL-6, IL-$1{\beta}$와 같은 전염증성 사이토카인들의 단백질 발현을 농도의존적으로 저해하였다. 이러한 결과들은 콜라비가 항산화 및 항염 효능을 가지는 소재로서의 개발 가능성이 있음을 시사한다.

Keywords

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