Abstract
This research was performed to develop mass propagation method of Alpine modest primrose (Primula modesta var. hannasanensis T.Yamaz.) for improving the conservation and utilization of the species. Seeds were collected on August 2011 in Jeju-city and well-selected seeds were dry-stored at $4{\pm}1.0^{\circ}C$. Seed size ranged $0.44{\pm}0.07{\times}0.61{\pm}0.04mm$, and weight of 1,000 seeds was $51.78{\pm}0.021mg$. Thus the seed was classified as 'dwarf seeds'. As the result of dormancy characteristics, moisture content of freshly matured seeds increased rapidly by water-soaking treatment and seeds did not germinate at $20^{\circ}C$ for 4 weeks under the light condition. Therefore, seeds were estimated to have physiological dormancy. Germination conditions of dry-stored seeds were found to be $20^{\circ}C$ and light condition, and seeds did not germinated under dark condition regardless of all temperature regimes. Therefore, Alpine modest primrose seed was considered as photoblastic type. Percent germination (PG) and germination energy (GE) were greatly improved by soaking the seeds in $GA_3$ and kinetin for 24 hours. Especially, 200~500 mg/L $GA_3$ treatment resulted in the highest PG (95.5%) and GE (98.3%). So, chemical treatment such as $GA_3$ was thought to be a useful method for raising seedling uniformly.
본 연구는 설앵초의 종 보존과 활용성 증대를 위해 종자를 이용한 대량 번식법을 개발하고자 수행되었다. 종자는 2011년 제주도 일대에서 채종하여 정선한 다음 $4{\pm}1.0^{\circ}C$에서 건조 상태로 저장하면서 연구에 사용하였다. 종자의 크기는 $0.44{\pm}0.07{\times}0.61{\pm}0.04mm$, 1,000립중은 $51.78{\pm}0.021mg$이었으며, 미세종자로 분류되었다. 설앵초 종자의 휴면특성은 생리적 휴면(physiological dormancy)성을 갖는 것으로 판단되었다. 한편 종자는 광조건에서 발아가 되는 광발아성 종자로, 적정온도는 $20^{\circ}C$로 나타났다. $GA_3$는 조자의 휴면타파 및 발아력 향상에 크게 효과가 있었는데, 농도가 높아줄수록 그 효과가 크게 나타났다. 특히 $GA_3$ 200~500 mg/L 처리구에서 발아율 및 발아세가 90% 이상으로 매우 높게 조사되었다.
