Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Bioconversion of Pinoresinol Diglucoside from Glucose Using Resting and Freeze-Dried Phomopsis sp. XP-8 Cells

  • Gao, Zhenhong (College of Food Science and Engineering, Northwest A & F University) ;
  • Rajoka, Muhammad Shahid Riaz (Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University) ;
  • Zhu, Jing (Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University) ;
  • Zhang, Zhiwei (College of Food Science and Engineering, Qingdao Agriculture University) ;
  • Zhang, Yan (College of Food Science and Engineering, Northwest A & F University) ;
  • Che, Jinxin (College of Food Science and Engineering, Northwest A & F University) ;
  • Xu, Xiaoguang (Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University) ;
  • Shi, Junling (Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University)
  • Received : 2017.03.24
  • Accepted : 2017.06.13
  • Published : 2017.08.28
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Abstract

Phomopsis sp. XP-8 (an endophytic fungus) was previously found to produce pinoresinol diglucoside (PDG), a major antihypertensive compound of Tu-Chung (the bark of Eucommia ulmoides Oliv.), which is widely used in Chinese traditional medicines. In the present study, two bioconversion systems were developed for the production of PDG in Tris-HCl buffer containing glucose and Phomopsis sp. XP-8 cells (both resting and freeze-dried). When other factors remained unchanged, the bioconversion time, glucose concentration, cell ages, cell dosage, pH, temperature, and stirring speed influenced PDG production in a similar and decreasing manner after an initial increase with increasing levels for each factor. Considering the simultaneous change of various factors, the optimal conditions for PDG production were established as 70 g/l cells (8-day-old), 14 g/l glucose, $28^{\circ}C$, pH 7.5, and 180 rpm for systems employing resting cells, and 3.87 g/l cells, 14.67 g/l glucose, $28^{\circ}C$, pH 7.5, and 180 rpm for systems employing freeze-dried cells. The systems employing freeze-dried cells showed lower peak PDG production ($110.28{\mu}g/l$), but at a much shorter time (12.65 h) compared with resting cells (23.62 mg/l, 91.5 h). The specific PDG production levels were 1.92 and $24{\mu}g$ per gram cells per gram glucose for freeze-dried cells and resting cells, respectively. Both systems indicated a new and potentially efficient way to produce PDG independent of microbial cell growth.

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References

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