Mycobiology

The Korean Society of Mycology (한국균학회)
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Optimization of Submerged Fermentation Medium for Matrine Production by Aspergillus terreus, an Endophytic Fungus Harboring Seeds of Sophora flavescens, Using Response Surface Methodology

  • Zhang, Qiang (School of Pharmacy, Shandong University of Traditional Chinese Medicine) ;
  • Li, Yujuan (School of Pharmacy, Shandong University of Traditional Chinese Medicine) ;
  • Xu, Fangxue (School of Pharmacy, Shandong University of Traditional Chinese Medicine) ;
  • Zheng, Mengmeng (School of Pharmacy, Shandong University of Traditional Chinese Medicine) ;
  • Xi, Xiaozhi (School of Pharmacy, Shandong University of Traditional Chinese Medicine) ;
  • Zhang, Xuelan (School of Pharmacy, Shandong University of Traditional Chinese Medicine) ;
  • Han, Chunchao (School of Pharmacy, Shandong University of Traditional Chinese Medicine)
  • Received : 2016.12.29
  • Accepted : 2017.06.15
  • Published : 2017.06.30
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Abstract

Different endophytes isolated from the seeds of Sophora flavescens were tested for their ability to produce matrine production. Response surface methodology (RSM) was applied to optimize the medium components for the endophytic fungus. Results indicated that endophyte Aspergillus terreus had the ability to produce matrine. The single factor tests demonstrated that potato starch was the best carbon source and the combination of peptone and $NH_4NO_3$ was the optimal nitrogen source for A. terreus. The model of RSM predicted to gain the maximal matrine production at $20.67{\mu}g/L$, when the potato starch was 160.68 g/L, peptone was 24.96 g/L and $NH_4NO_3$ was 2.11 g/L. When cultured in the optimal medium, the matrine yield was an average of $20.63{\pm}0.11{\mu}g/L$, which was consistent with the model prediction. This study offered an alternative source for the matrine production by endophytic fungus fermentation and may have far-reaching prospect and value.

Keywords

References

  1. Venugopalan A, Potunuru UR, Dixit M, Srivastava S. Effect of fermentation parameters, elicitors and precursors on camptothecin production from the endophyte Fusarium solani. Bioresour Technol 2016;206:104-11. https://doi.org/10.1016/j.biortech.2016.01.079
  2. Tan RX, Zou WX. Endophytes: a rich source of functional metabolites. Nat Prod Rep 2001;18:448-59. https://doi.org/10.1039/b100918o
  3. Strobel G, Daisy B, Castillo U, Harper J. Natural products from endophytic microorganisms. J Nat Prod 2004;67:257-68. https://doi.org/10.1021/np030397v
  4. Yang SX, Wang HP, Gao JM, Zhang Q, Laatsch H, Kuang Y. Fusaroside, a unique glycolipid from Fusarium sp., an endophytic fungus isolated from Melia azedarach. Org Biomol Chem 2012;10:819-24. https://doi.org/10.1039/C1OB06426F
  5. Wu B, Wu L, Ruan L, Ge M, Chen D. Screening of endophytic fungi with antithrombotic activity and identification of a bioactive metabolite from the endophytic fungal strain CPCC 480097. Curr Microbiol 2009;58:522-7. https://doi.org/10.1007/s00284-009-9361-7
  6. Kucukboyaci N, Ozkan S, Adiguzel N, Tosun F. Characterisation and antimicrobial activity of Sophora alopecuroides L. var. alopecuroides alkaloid extracts. Turk J Biol 2011;35:379-85.
  7. Guo Z, Zhang L, Song C, Zhang X. Molecularly imprinted solid-phase extraction of matrine from radix Sophorae tonkinensis. Analyst 2011;136:3016-22. https://doi.org/10.1039/c1an15281e
  8. Fu Q, Fang Q, Feng B, Sun S, Du W, Amut E, Xiao A, Chang C. Matrine-imprinted monolithic stationary phase for extraction and purification of matrine from Sophorae flavescentis Ait. J Chromatogr B Analyt Technol Biomed Life Sci 2011;879:894-900. https://doi.org/10.1016/j.jchromb.2011.02.041
  9. Li H, Xie S, Liu X, Wu H, Lin X, Gu J, Wang H, Duan Y. Matrine alters microRNA expression profiles in SGC-7901 human gastric cancer cells. Oncol Rep 2014;32:2118-26. https://doi.org/10.3892/or_xxxxxxxx
  10. Shao H, Yang B, Hu R, Wang Y. Matrine effectively inhibits the proliferation of breast cancer cells through a mechanism related to the NF-${\kappa}B$ signaling pathway. Oncol Lett 2013;6: 517-20. https://doi.org/10.3892/ol.2013.1399
  11. Yang Y, Xiu J, Zhang X, Zhang L, Yan K, Qin C, Liu J. Antiviral effect of matrine against human enterovirus 71. Molecules 2012;17:10370-6. https://doi.org/10.3390/molecules170910370
  12. Li XM, Brown L. Efficacy and mechanisms of action of traditional Chinese medicines for treating asthma and allergy. J Allergy Clin Immunol 2009;123:297-306. https://doi.org/10.1016/j.jaci.2008.12.026
  13. Kan QC, Zhu L, Liu N, Zhang GX. Matrine suppresses expression of adhesion molecules and chemokines as a mechanism underlying its therapeutic effect in CNS autoimmunity. Immunol Res 2013;56:189-96. https://doi.org/10.1007/s12026-013-8393-z
  14. Rafigh SM, Yazdi AV, Vossoughi M, Safekordi AA, Ardjmand M. Optimization of culture medium and modeling of curdlan production from Paenibacillus polymyxa by RSM and ANN. Int J Biol Macromol 2014;70:463-73. https://doi.org/10.1016/j.ijbiomac.2014.07.034
  15. Jia J, Yang X, Wu Z, Zhang Q, Lin Z, Guo H, Lin CS, Wang J, Wang Y. Optimization of fermentation medium for extracellular lipase production from Aspergillus niger using response surface methodology. Biomed Res Int 2015;2015:497462.
  16. Jiapeng T, Yiting L, Li Z. Optimization of fermentation conditions and purification of cordycepin from Cordyceps militaris. Prep Biochem Biotechnol 2014;44:90-106. https://doi.org/10.1080/10826068.2013.833111
  17. Singh NK, Parmar A, Madamwar D. Optimization of medium components for increased production of C-phycocyanin from Phormidium ceylanicum and its purification by single step process. Bioresour Technol 2009;100:1663-9. https://doi.org/10.1016/j.biortech.2008.09.021
  18. He L. Isolation and identification of the antagonistic substance of the endophytic fungi from Sophora flavescens and its function mechanism. Shenyang: Shenyang Agricultural University; 2011.
  19. Li K, Wang H. Simultaneous determination of matrine, sophoridine and oxymatrine in Sophora flavescens Ait. by high performance liquid chromatography. Biomed Chromatogr 2004;18:178-82. https://doi.org/10.1002/bmc.308
  20. Wang H, Li G, Zhang W, Han C, Xu X, Li YP. The protective effect of Agaricus blazei Murrill, submerged culture using the optimized medium composition, on alcohol-induced liver injury. Biomed Res Int 2014;2014:573978.
  21. Lu CH, Engelmann NJ, Lila MA, Erdman JW Jr. Optimization of lycopene extraction from tomato cell suspension culture by response surface methodology. J Agric Food Chem 2008;56: 7710-4. https://doi.org/10.1021/jf801029k
  22. Stierle A, Strobel G, Stierle D. Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew. Science 1993;260:214-6. https://doi.org/10.1126/science.8097061
  23. Macias-Rubalcava ML, Hernandez-Bautista BE, Jimenez- Estrada M, Gonzalez MC, Glenn AE, Hanlin RT, Hernandez- Ortega S, Saucedo-Garcia A, Muria-Gonzalez JM, Anaya AL. Naphthoquinone spiroketal with allelochemical activity from the newly discovered endophytic fungus Edenia gomezpompae. Phytochemistry 2008;69:1185-96. https://doi.org/10.1016/j.phytochem.2007.12.006
  24. Arora D, Sharma N, Singamaneni V, Sharma V, Kushwaha M, Abrol V, Guru S, Sharma S, Gupta AP, Bhushan S, et al. Isolation and characterization of bioactive metabolites from Xylaria psidii, an endophytic fungus of the medicinal plant Aegle marmelos and their role in mitochondrial dependent apoptosis against pancreatic cancer cells. Phytomedicine 2016; 23:1312-20. https://doi.org/10.1016/j.phymed.2016.07.004
  25. Liu L, Han Y, Xiao J, Li L, Guo L, Jiang X, Kong L, Che Y. Chlorotheolides A and B, spiroketals generated via diels-alder reactions in the endophytic fungus Pestalotiopsis theae. J Nat Prod 2016;79:2616-23. https://doi.org/10.1021/acs.jnatprod.6b00550
  26. Xiao Z, Wang X, Huang Y, Huo F, Zhu X, Xi L, Lu JR. Thermophilic fermentation of acetoin and 2,3-butanediol by a novel Geobacillus strain. Biotechnol Biofuels 2012;5:88. https://doi.org/10.1186/1754-6834-5-88
  27. Zhang Q, Liu Y, Di Z, Han C, Liu Z. The strategies for increasing cordycepin production of Cordyceps militaris by liquid fermentation. Fungal Genom Biol 2016;6:134.
  28. Mao XB, Eksriwong T, Chauvatcharin S, Zhong JJ. Optimization of carbon source and carbon/nitrogen ratio for cordycepin production by submerged cultivation of medicinal mushroom Cordyceps militaris. Process Biochem 2005;40:1667-72. https://doi.org/10.1016/j.procbio.2004.06.046
  29. Zong H, He Y, Zhan Y, Du J, Feng F, Li D. Optimization of medium constituents for ${\varepsilon}$-poly-L-lysine fermentation with response surface methodology. J Food Sci 2010;75:M552-6. https://doi.org/10.1111/j.1750-3841.2010.01820.x
  30. Mao XB, Zhong JJ. Significant effect of $NH_4^+$ on cordycepin production by submerged cultivation of medicinal mushroom Cordyceps militaris. Enzyme Microb Technol 2006;38:343-50. https://doi.org/10.1016/j.enzmictec.2004.10.010
  31. Masuda M, Urabe E, Sakurai A, Sakakibara M. Production of cordycepin by surface culture using the medicinal mushroom Cordyceps militaris. Enzyme Microb Technol 2006;39:641-6. https://doi.org/10.1016/j.enzmictec.2005.11.010
  32. Tanyol M, Uslu G, Yonten V. Optimization of lipase production on agro-industrial residue medium by Pseudomonas fluorescens (NRLL B-2641) using response surface methodology. Biotechnol Biotechnol Equip 2015;29:64-71. https://doi.org/10.1080/13102818.2014.991635
  33. Shende D, Sidhu GK. Response surface methodology to optimize enzyme-assisted aqueous extraction of maize germ oil. J Food Sci Technol 2016;53:3282-95. https://doi.org/10.1007/s13197-016-2303-z
  34. Momen SB, Siadat SD, Akbari N, Ranjbar B, Khajeh K. Applying central composite design and response surface methodology to optimize growth and biomass production of Haemophilus influenzae type b. Jundishapur J Microbiol 2016;9:e25246.
  35. Raza A, Li F, Xu X, Tang J. Optimization of ultrasonic-assisted extraction of antioxidant polysaccharides from the stem of Trapa quadrispinosa using response surface methodology. Int J Biol Macromol 2016;94(Pt A):335-44.
  36. Murthy MS, Swaminathan T, Rakshit SK, Kosugi Y. Statistical optimization of lipase catalyzed hydrolysis of methyloleate by response surface methodology. Bioprocess Eng 2000;22:35-9. https://doi.org/10.1007/PL00009097
  37. Feng YL, Li WQ, Wu XQ, Cheng JW, Ma SY. Statistical optimization of media for mycelial growth and exopolysaccharide production by Lentinus edodes and a kinetic model study of two growth morphologies. Biochem Eng J 2010;49:104-12. https://doi.org/10.1016/j.bej.2009.12.002
  38. Das SK, Masuda M, Hatashita M, Sakurai A, Sakakibara M. A new approach for improving cordycepin productivity in surface liquid culture of Cordyceps militaris using high-energy ion beam irradiation. Lett Appl Microbiol 2008;47:534-8. https://doi.org/10.1111/j.1472-765X.2008.02456.x