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Sugar content analysis and expression profiling of sugar related genes in contrasting Strawberry (Fragaria × ananassa) cultivars

  • Received : 2017.06.09
  • Accepted : 2017.06.30
  • Published : 2017.06.30

Abstract

$Fragaria{\times}ananassa$, a strawberry evolved from hybridization between F. virginiana and F. chiloensis, is a globally cultivated and consumed fruit crop valued for its flavor and nutritional value. Flavor and quality of fruits are determined by factors such as sugars and organic acids present during fruit development. These characteristics are highly subjective in different genotypes and affected by various environmental factors. In this study, we analyzed contents of major sugar compounds including fructose, glucose and sucrose by HPLC analysis in four cultivars namely, Maehyang, Seolhyang, Festival and Sweet Charlie. We identified 55 genes related to fructose, glucose, sucrose and soluble sugar regulation whose expression were analyzed in four cultivars at three developmental stages of the fruit namely, green, white and ripened stages. Expression of these genes across these progressive fruit developmental stages varied among cultivars. Among the 55 genes, genes FaFru3, FaSuc11 and FaGlu8 revealed differential patterns of expression along developmental stages of the fruit in high and low sugar-containing genotypes, respectively and may be putative candidates for sugar content in strawberries. Expression of genes are discussed with regard to corresponding sugar content in these genotypes. Further analysis and application of these genes may be valuable in developing high sugar containing cultivars via marker-assisted breeding.

Keywords

References

  1. Amil-Ruiz F, Garrido-Gala J, Blanco-Portales R, et al. (2013) Identification and validation of reference genes for transcript normalization in strawberry (Fragaria x ananassa) defense responses. PLoS One 8:e70603 https://doi.org/10.1371/journal.pone.0070603
  2. Basson CE, Groenewald JH, Kossmann J, et al. (2010) Sugar and acid-related quality attributes and enzyme activities in strawberry fruits: Invertase is the main sucrose hydrolysing enzyme. Food Chem 121:1156-1162 https://doi.org/10.1016/j.foodchem.2010.01.064
  3. Basu A, Nguyen A, Betts NM, Lyons TJ (2014) Strawberry As a Functional Food: An Evidence-Based Review. Crit Rev Food Sci Nutr 54:790-806 https://doi.org/10.1080/10408398.2011.608174
  4. Bestfleisch M, Mohring J, Hanke MV, et al. (2014) A diallel crossing approach aimed on selection for ripening time and yield in breeding of new strawberry (Fragaria $\times$ ananassa Duch.) cultivars. Plant Breed 133:115-120 https://doi.org/10.1111/pbr.12120
  5. Crespo P, Gine Bordonaba J, Terry LA, Carlen C (2010) Characterisation of major taste and health-related compounds of four strawberry genotypes grown at different Swiss production sites. Food Chem 122:16-24 https://doi.org/10.1016/j.foodchem.2010.02.010
  6. Galli V, Borowski JM, Perin EC, et al. (2015) Validation of reference genes for accurate normalization of gene expression for real time-quantitative PCR in strawberry fruits using different cultivars and osmotic stresses. Gene 554:205-214 https://doi.org/10.1016/j.gene.2014.10.049
  7. Giampieri F, Alvarez-Suarez JM, Mazzoni L, et al. (2013) The potential impact of strawberry on human health. Nat Prod Res 27:448-455 https://doi.org/10.1080/14786419.2012.706294
  8. Gunduz K, Ozdemir E (2014) The effects of genotype and growing conditions on antioxidant capacity, phenolic compounds, organic acid and individual sugars of strawberry. Food Chem 155:298-303 https://doi.org/10.1016/j.foodchem.2014.01.064
  9. Jia H, Jiu S, Zhang C, et al. (2016) Abscisic acid and sucrose regulate tomato and strawberry fruit ripening through the abscisic acid-stress-ripening transcription factor. Plant Biotechnol J 14:2045-2065 https://doi.org/10.1111/pbi.12563
  10. Jia H, Wang Y, Sun M, et al. (2013) Sucrose functions as a signal involved in the regulation of strawberry fruit development and ripening Sucrose functions as a signal involved in the regulation of strawberry fruit development and ripening. New Phytol 453-465
  11. Kafkas E, Kosar M, Paydas S, et al. (2007) Quality characteristics of strawberry genotypes at different maturation stages. Food Chem 100:1229-1236 https://doi.org/10.1016/j.foodchem.2005.12.005
  12. Kallio H, Hakala M, Pelkkikangas M, Lapvetel. (2000) Sugars and acids of strawberry varieties. Eur Food Res Technol 212:81-85 https://doi.org/10.1007/s002170000244
  13. Kim DaeYoung; Yoon MooKyung; Kwak JungHo; Kim TaeIl and Kim JinHan (2009) Classification of strawberry germplasms based on horticultural traits and principal component analysis. Korean J Hortic Sci Technol 27:636-643
  14. Macias-Rodriguez L, Quero E, Lopez MG (2002) Carbohydrate differences in strawberry crowns and fruit (Fragaria x ananassa) during plant development. J Agric Food Chem 50:3317-21 https://doi.org/10.1021/jf011491p
  15. Marchler-Bauer A, Derbyshire MK, Gonzales NR, et al. (2015) CDD: NCBI's conserved domain database. Nucleic Acids Res 43:D222-D226 https://doi.org/10.1093/nar/gku1221
  16. Mishra PK, Ram RB, Kumar N, et al. (2015) Physico-chemical characteristics of some strawberry (Fragaria x ananassa Duch.) genotypes. Intern ional J Multidiscip Res Dev Vol 2:216-218
  17. Molina-Hidalgo FJ, Franco AR, Villatoro C, et al. (2013) The strawberry (Fragaria x ananassa) fruit-specific rhamnogalacturonate lyase 1 (FaRGLyase1) gene encodes an enzyme involved in the degradation of cell-wall middle lamellae. J Exp Bot 64:1471-1483 https://doi.org/10.1093/jxb/ers386
  18. Natarajan S, Kim H, Thamilarasan SK, et al. (2016) Whole Genome Re-Sequencing and Characterization of Powdery Mildew Disease-Associated Allelic Variation in Melon. PLoS One 11:e0157524 https://doi.org/10.1371/journal.pone.0157524
  19. Shanmugam A, Thamilarasan SK, Park J-I, et al. (2016) Characterization and abiotic stress-responsive expression analysis of SGT1 genes in Brassica oleracea. Genome 59:243-251 https://doi.org/10.1139/gen-2015-0128
  20. Sturm K, Koron D, Stampar F (2003) The composition of fruit of different strawberry varieties depending on maturity stage. Food Chem 83:417-422 https://doi.org/10.1016/S0308-8146(03)00124-9
  21. Vijayakumar H, Thamilarasan SK, Shanmugam A, et al. (2016) Glutathione Transferases Superfamily: Cold-Inducible Expression of Distinct GST Genes in Brassica oleracea. Int J Mol Sci 17:1211 https://doi.org/10.3390/ijms17081211
  22. Wang, Shiow Y., Lin H-S (2000) Antioxidant activity in fruits and leaves. J Agric Food Chem 48:140-146 https://doi.org/10.1021/jf9908345
  23. Zhongjie LIU, Pengcheng Z, Liwen CUI, et al. (2016) Over-expression of Gene FaASR Promotes Strawberry Fruit Coloring. Hortic Plant J 1:147-154

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