미생물학회지 (Korean Journal of Microbiology)

  • 제53권2호
  • /
  • Pages.146-148
  • /
  • 2017
  • /
  • 0440-2413(pISSN)
  • /
  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지
DOI QR코드

DOI QR Code

비육돈 분변으로부터 분리한 Lactobacillus reuteri KLR3004 유산균주의 유전체 분석

Draft genome sequence of Lactobacillus reuteri KLR3004 from a fattening pig

  • 박종빈 (강원대학교 동물생명과학과) ;
  • 이준영 (서울대학교 농생명공학부) ;
  • 진귀득 (강원대학교 동물생명과학과) ;
  • 김은배 (강원대학교 동물생명과학과)
  • Park, Jongbin (Department of Animal Life Science, Kangwon National University) ;
  • Lee, Jun-Yeong (Department of Agricultural Biotechnology, Seoul National University) ;
  • Jin, Gwi-Deuk (Department of Animal Life Science, Kangwon National University) ;
  • Kim, Eun Bae (Department of Animal Life Science, Kangwon National University)
  • 투고 : 2017.05.10
  • 심사 : 2017.06.27
  • 발행 : 2017.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

국내 비육돈에서 분리된 Lactobacillus reuteri KLR3004 유산균주의 분석을 실시하였다. 이 유전체 초안은 크기가 1,996,237 bp이며 G+C content (%)는 38.75%이다. 이 초안속의 149개 contig들로부터 1,837개의 단백질 코딩 유전자가 예측되었다.

We sequenced the genome of Lactobacillus reuteri KLR3004 strain isolated from a fattening pig in South Korea. The sequences were assembled into a draft genome containing 1,996,237 bp with a G+C content of 38.75% and 1,837 predicted protein-coding sequences in 149 contigs.

키워드

참고문헌

  1. Ahn, J.S., Park, B.K., Kim, Y.J., Hong, B.C., Ra, C.S., Kim, M.J., and Shin, J.S. 2016. Effects of fermentation using microorganisms on feed value of Astragalus membranaceus by-products. Ann. Anim. Resour. Sci. 27, 44-56. https://doi.org/10.12718/AARS.2016.27.1.44
  2. Bankevich, A., Nurk, S., Antipov, D., Gurevich, A.A., Dvorkin, M., Kulikov, A.S., Lesin, V.M., Nikolenko, S.I., Pham, S., Prjibelski, A.D., et al. 2012. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J. Comput. Biol. 19, 455-477. https://doi.org/10.1089/cmb.2012.0021
  3. Chiang, M.L., Chen, H.C., Chen, K.N., Lin, Y.C., Lin, Y.T., and Chen, M.J. 2015. Optimizing production of two potential probiotic lactobacilli strains isolated from piglet feces as feed additives for weaned piglets. Asian-Australas J. Anim. Sci. 28, 1163-1170. https://doi.org/10.5713/ajas.14.0780
  4. Economou, V. and Gousia, P. 2015. Agriculture and food animals as a source of antimicrobial-resistant bacteria. Infect. Drug Resist. 8, 49-61.
  5. Grissa, I., Vergnaud, G., and Pourcel, C. 2007. CRISPRFinder: a web tool to identify clustered regularly interspaced short palindromic repeats. Nucleic Acids Res. 35, W52-57. https://doi.org/10.1093/nar/gkm360
  6. Mackenzie, D.A., McLay, K., Roos, S., Walter, J., Swarbreck, D., Drou, N., Crossman, L.C., and Juge, N. 2014. Draft genome sequence of a novel Lactobacillus salivarius strain isolated from piglet. Genome Announc. 2, e01231-01213.
  7. Martin, M. 2011. Cutadapt removes adapter sequences from highthroughput sequencing reads. EMBnet. J. 17.
  8. McArthur, A.G., Waglechner, N., Nizam, F., Yan, A., Azad, M.A., Baylay, A.J., Bhullar, K., Canova, M.J., De Pascale, G., Ejim, L., et al. 2013. The comprehensive antibiotic resistance database. Antimicrob. Agents Chemother. 57, 3348-3357. https://doi.org/10.1128/AAC.00419-13
  9. Overbeek, R., Olson, R., Pusch, G.D., Olsen, G.J., Davis, J.J., Disz, T., Edwards, R.A., Gerdes, S., Parrello, B., Shukla, M., et al. 2014. The SEED and the rapid annotation of microbial genomes using Subsystems Technology (RAST). Nucleic Acids Res. 42, D206-214. https://doi.org/10.1093/nar/gkt1226
  10. Sung, H.G. 2013. Effects of fermented phytogenics on fecal odour and performance in laying hens. Ann. Anim. Resour. Sci. 24, 29-37. https://doi.org/10.12718/AARS.2013.24.1.29
  11. Yeo, S., Lee, S., Park, H., Shin, H., Holzapfel, W., and Huh, C.S. 2016. Development of putative probiotics as feed additives: validation in a porcine-specific gastrointestinal tract model. Appl. Environ. Microbiol. 100, 10043-10054.