미생물학회지 (Korean Journal of Microbiology)

  • 제53권2호
  • /
  • Pages.144-145
  • /
  • 2017
  • /
  • 0440-2413(pISSN)
  • /
  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지
DOI QR코드

DOI QR Code

청국장에서 분리된 신생 박테리오파지 SPG24의 전체 염기 서열

Complete genome sequence of a novel bacteriophage SPG24 isolated from Cheonggukjang

  • 김채현 (충북대학교 자연과학대학 미생물학과) ;
  • 이규철 (한국수자원공사 수질연구센터 미생물과) ;
  • 김인교 (충북대학교 자연과학대학 미생물학과) ;
  • 김석천 (충북대학교 자연과학대학 미생물학과) ;
  • 이오형 (목포대학교 자연과학대학 생명과학과) ;
  • 이찬희 (충북대학교 자연과학대학 미생물학과)
  • Kim, Chaehyun (Department of Microbiology, College of Natural Sciences, Chungbuk National University) ;
  • Lee, Gyu-Cheol (Department of Microbiology, Water Quality Research Center, K-water) ;
  • Kim, In Kyo (Department of Microbiology, College of Natural Sciences, Chungbuk National University) ;
  • Kim, Seok Cheon (Department of Microbiology, College of Natural Sciences, Chungbuk National University) ;
  • Lee, Oh Hyung (Department of Bio Sciences, College of Natural Science, Mokpo National University) ;
  • Lee, Chan Hee (Department of Microbiology, College of Natural Sciences, Chungbuk National University)
  • 투고 : 2017.06.08
  • 심사 : 2017.06.08
  • 발행 : 2017.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Bacillus subtilis를 이용하여 청국장 발효 연구를 진행하던 중 발효능이 뛰어난 B. subtilis G24라는 균주를 새로 분리하였고, 이를 숙주로 이용하는 bacteriophage SPG24를 새로 분리하였다. 본 연구에서는 bacteriophage SPG24의 유전체 서열을 해독하였으며 전체길이 152,060 bp, G+C 함량 42.2%, 232개 ORF의 bacteriophage SPG24는 B. subtilis G24를 숙주로 이용함으로써 청국장 발효를 저해하는 것으로 확인되었다.

A Bacillus subtilis strain, designated G24, was isolated from grape during a study on the fermentation of Cheongkukjang, Korean traditional fast-fermented bean paste. Also, a newly isolated bacteriophage SPG24, which was found to inhibit the fermentation process, uses B. subtilis G24 as a host. The complete genome sequence of the bacteriophage SPG24 was 152,060 bp in length, with a G+C content of 42.2%. This sequence included 232 ORF; 58 forward ORFs and 174 reverse ORFs.

키워드

참고문헌

  1. Ackermann, H.W. 1974. Classification of Bacillus and Clostridium bacteriophage. Pathol. Biol. (Paris) 22, 909-917.
  2. Hatful, G.F. 2008. Bacteriophage genomics. Curr. Opin. Microbiol. 11, 447-453. https://doi.org/10.1016/j.mib.2008.09.004
  3. Lee, J.H., Shin, H., Son, B., and Ryu, S. 2012. Complete genome sequence of Bacillus cereus bacteriophage BCP78. J. Virol. 86, 637-638. https://doi.org/10.1128/JVI.06520-11
  4. Park, Y.S. 2006. Characterization of Bacillus subtilis phage SPG24 and its relationship to host infectivity. Mokpo National University, Master degree thesis. Retrieved from National Library of Korea (Accession no. DM475. 163-6-6=2).
  5. Pires, D.P., Kropinski, A.M., Azeredo, J., and Sillankorva, S. 2014. Complete genome sequence of the Pseudomonas aeruginosa bacteriophage phiIBB-PAA2. Genome Announc. 2, e01102-01113.
  6. Suttle, C.A. 2007. Marine viruses-major players in the global ecosystem. Nat. Rev. Mcirobiol. 5, 801-812 https://doi.org/10.1038/nrmicro1750