Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation of Bacillus sp. SW29-2 and Its Antifungal Activity against Colletotrichum coccodes

Bacillus sp. SW29-2의 분리 및 Colletotrichum coccodes에 대한 항진균 활성

  • Received : 2016.12.16
  • Accepted : 2017.02.09
  • Published : 2017.06.30
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Abstract

Antifungal bacterium against Colletotrichum coccodes causing black dot disease of potatoes and anthracnose of tomatoes was isolated from sewage sludge. The isolate showed a 99% sequence homology of partial 16S rRNA of Bacillus methylotrophicus CBMB205 and Bacillus amyloliquefaciens subsp. plantarum FZB42. The isolate was identified as Bacillus sp. SW29-2, using the neighbor-joining phylogenetic tree, BlastN sequence analysis, and morphological and cultural characteristics. Bacillus sp. SW29-2 is an aerobic, Gram-positive, endospore-forming bacterium, of which the morphological and physiological characteristics were the same as those of type strain B. lichniformis CBMB205, except for the cell growth of over 4% NaCl. The cell growth of the temperature and the initial pH of the medium was shown at $18-47^{\circ}C$ (opt. ca. $38^{\circ}C$) and 3-9 (opt. ca. 6.0), respectively. The inhibition size (diameter) of Bacillus sp. SW29-2 against four strains of C. coccodes ranged from 23 to 29 mm. Also, the isolate showed antifungal activity against penicillium rot-causing Penicillium expansum in apples. Thus far, any report on the antifungal activity of Baciilus spp. against C. coccodes has not been found. These results suggest that the Bacillus sp. SW29-2 isolate could be used as a possible biocontrol agent against C. coccodes, and further applied to other plant pathogenic fungi.

하수 슬러지로부터 감자 뿌리썩음병 및 토마토 탄저병을 유발하는 Colletotrichum coccodes에 대해 항진균 활성이 있는 세균을 분리하였다. 분리균의 partial 16S rRNA sequence는 Bacillus methylotrophicus CBMB205 및 Bacillus amyloliquefaciens subsp. plantarum FZB42와 각각 99%의 상동성을 보여주었다. 분리균은 neighbor-joining phylogenetic tree, BlastN 염기서열 분석, 형태학적 및 배양학적 특성에 따라 Bacillus sp. SW29-2로 동정하였다. 분리균 Baciilus sp. SW29-2는 B. methylotrophicus CBMB205 type strain과 비교시 4% 이상 NaCl에서 생육하는 것이외에 형태학적, 생리학적 특성이 모두 동일하였다. 분리은 호기성, Gram-양성, 내생포자를 형성하는 세균이었다. 실험한 $18-47^{\circ}C$(최적, 약 $38^{\circ}C$)의 온도범위에서, pH 3-9(최적, 약 6.0) 범위의 초기 배지 pH에서 모두 생육이 관찰되었다. Baciilus sp. SW29-2의 C. coccodes에 대한 생육저지환(직경)은 23 mm에서 29 mm의 범위이었다. 분리 세균은 또한 사과 푸른곰팡이병(penicillum rot disease)을 유발하는 Penicillium expansum에 대해 우수한 항진균 활성을 보여주었다. 현재까지, Bacillus속 세균의 C. coccodes에 대한 항균활성 연구는 파악할 수 없었다. 이 결과는 분리균 Bacillus sp. SW29-2가 식물 병원성 C. coccodes에 대해 생물학적 방제제로의 활용 가능성이 있으며, 추후, 식물병을 유발하는 다른 진균에 대해서 적용할 예정이다.

Keywords

References

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