• The Korean Journal of Mycology
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• v.46 no.2
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• pp.186-192
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• 2018

Rapid and accurate detection methods for Colletotrichum coccodes, an anthracnose pathogen of pepper and tomato, were developed using PCR. A specific primer set, coccoTef-F/coccoTef-R, which was constructed by analyzing tef-$1{\alpha}$ genes from 13 species and 22 strains of Colletotrichum, could specifically detect C. coccodes at a level of 10 ng by conventional PCR method and at 10 pg by real-time PCR. The PCR-based methods were also capable of detecting C. coccodes in pepper and tomato seeds artificially infected with the pathogen. The developed PCR methods can be applied for rapid and accurate inspection of C. coccodes in the seeds intended for export or import.

• The Korean Journal of Mycology
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• v.25 no.1 s.80
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• pp.6-9
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• 1997

Black dot root rot of eggplant was first found in vinyl-housed fields of Yeoju area in Korea in May, 1993. The causal fungus of the disease was identified as Colletotrichum coccodes (Wallr.) Hughes based on the morphological and cultural characteristics. Pathogenicity tests revealed that isolates of the fungus were responsible for the disease.

• Korean Journal Plant Pathology
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• v.14 no.3
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• pp.209-211
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• 1998

Black dot root rot occurred severely in greenhouse tomatoes in jangseong area of Korea in April, 1996. The causal fungus of the disease was identified as Colletotrichum coccodes based on the morphological and cultural characteristics. Pathogenicity tests revealed that isolates of the fungus were responsible for the disease, and two cultivars of cherry tomato were less susceptible to the disease than other cultivars of tomato and cherry tomato tested.

• Korean Journal Plant Pathology
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• v.14 no.5
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• pp.543-544
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• 1998

An anthracnose on potato leaves was observed in Kangwon alpine and Kangnung areas in Korea. A fungal pathogen was repeatedly isolated from the leaf lesions and stems of the infected plants and identified as Colletotrichum coccodes. The fungus showed pathogenicity on the leaves of potato inoculated. This is the first report that anthracnose of potato caused by Colletotrichum coccodes was occurred in Korea.

• The Korean Journal of Mycology
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• v.15 no.3
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• pp.183-186
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• 1987

Colletotrichum coccodes generally regarded as soil borne, was for the first time observed in seed samples of pepper(Capsicum annuum). Detailed descriptions were given on the habit character of the fungus and the morphology of conidia. The fungus caused anthracnose on fruits of pepper, tomato and egg plant under laboratory conditions.

• The Plant Pathology Journal
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• v.27 no.1
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• pp.1-7
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• 2011

Colletotrichum panacicola isolates were obtained from anthracnose lesions of Korean ginseng and compared with four Colletotrichum species in morphology, molecular phylogeny and pathogenicity. Based on morphological characteristics, C. panacicola was easily distinguished from Colletotrichum gloeosporioides but not from Colletotrichum higginsianum, Colletotrichum destructivum and Colletotrichum coccodes. A phylogenetic tree generated from ribosomal DNA-internal transcribed spacer sequences revealed that C. panacicola is remarkably distinguished from C. gloeosporioides and C. coccodes but not from C. higginsianum and C. destructivum. However, molecular sequence analysis of three combined genes (actin + elongation factor-$1{\alpha}$ + glutamine synthatase) provided sufficient variability to distinguish C. panacicola from other Colletotrichum species. Pathogencity tests showed that C. panacicola is pathogenic to Korean ginseng but not to other plants. These results suggest that C. panacicola is an independent taxon distin-zguishable from C. gloeosporioides and other morphologically similar Colletotrichum species.

• Research in Plant Disease
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• v.18 no.1
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• pp.62-64
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• 2012

Anthracnose was occurred on black raspberry (Rubus coreanus) grown at Gochang areas of Korea during the period of 2003-2009. Black raspberry was afflicted by anthracnose with initial lesion of dark brown circular spots on the naturally infected stems and petioles. The irregular spots was either fused or progressed into circular or elliptical shape. The causal fungi of anthracnose were isolated from the diseased plants and identified as Colltotrichum gloeosporioides, C. coccodes and C. acutatum based on the morphological and cultural characteristics. All isolates of C. gloeosporioides, C. coccodes and C. acutatum produced similar symptoms under pathogenic examination through artificial inoculation.

• Journal of Life Science
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• v.27 no.6
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• pp.688-693
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• 2017

Antifungal bacterium against Colletotrichum coccodes causing black dot disease of potatoes and anthracnose of tomatoes was isolated from sewage sludge. The isolate showed a 99% sequence homology of partial 16S rRNA of Bacillus methylotrophicus CBMB205 and Bacillus amyloliquefaciens subsp. plantarum FZB42. The isolate was identified as Bacillus sp. SW29-2, using the neighbor-joining phylogenetic tree, BlastN sequence analysis, and morphological and cultural characteristics. Bacillus sp. SW29-2 is an aerobic, Gram-positive, endospore-forming bacterium, of which the morphological and physiological characteristics were the same as those of type strain B. lichniformis CBMB205, except for the cell growth of over 4% NaCl. The cell growth of the temperature and the initial pH of the medium was shown at $18-47^{\circ}C$ (opt. ca. $38^{\circ}C$) and 3-9 (opt. ca. 6.0), respectively. The inhibition size (diameter) of Bacillus sp. SW29-2 against four strains of C. coccodes ranged from 23 to 29 mm. Also, the isolate showed antifungal activity against penicillium rot-causing Penicillium expansum in apples. Thus far, any report on the antifungal activity of Baciilus spp. against C. coccodes has not been found. These results suggest that the Bacillus sp. SW29-2 isolate could be used as a possible biocontrol agent against C. coccodes, and further applied to other plant pathogenic fungi.

• Journal of Microbiology and Biotechnology
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• v.16 no.2
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• pp.280-285
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• 2006

Methanol extract of the rhizomes of turmeric, Curcuma longa L., effectively controlled the development of red pepper anthracnose caused by Colletotrichum coccodes. In addition three antifungal substances were identified from the methanol extract of C. longa rhizomes as curcumin, demethoxycurcumin, and bisdemethoxycurcumin using mass and $^{1}H-NMR$ spectral analyses. The curcuminoids in a range $0.4-100\;{\mu}g/ml$ effectively inhibited the mycelial growth of three red pepper anthracnose pathogens, C. coccodes, C. gloeosporioides, and C. acutatum. The three curcuminoids inhibited mycelial growth of C. coccodes and C. gloeosporioides to an extent similar to the synthetic fungicide dithianon did, but the synthetic agent was a little more effective against C. acutatum. The curcuminoids also effectively inhibited spore germination of C. coccodes, and bisdemethoxycurcumin was the most active. Among the three curcuminoids, only demethoxycurcumin was effective in a greenhouse test in suppressing red pepper anthracnose caused by C. coccodes.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.132.2-132
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• 2003

Anthracnose occurred on rapsberry grown in Gochang areas of Korea in 2003. The disease incidence was ranged from 1.1 to 2.6％. Anthracnose of rapsberry appeared as dark brown circular spots on naturally infected stems. The symptoms of infected stems were small brown to dark brown spots and gradually enlarged larger cylindrical dark brown lesions. The causal fungus of anthracnose isolated from the diseased plants was identifed as Colletotrichum coccodes based on the morphological and cultural characteristics. All isolates of C. coccodes were produced similar symptoms on the host leaves by artificial inocultion.