DOI QR코드

DOI QR Code

Beauty food activities of extracts from Pinus densiflora root

동송근(Pinus densiflora root) 추출물의 미용식품활성

  • Lee, Eun-Ho (School of Food science & Biotechnology/Food & Bio-Industry Research Institute, Kyungpook National University) ;
  • Park, Ki-Tae (School of Culinary Art and Baking technology, Dongju College University) ;
  • Park, Hye-Jin (School of Food science & Biotechnology/Food & Bio-Industry Research Institute, Kyungpook National University) ;
  • Jo, Jae-Bum (School of Food science & Biotechnology/Food & Bio-Industry Research Institute, Kyungpook National University) ;
  • Lee, Jae-Eun (School of Food science & Biotechnology/Food & Bio-Industry Research Institute, Kyungpook National University) ;
  • Lim, Su-Bin (School of Food science & Biotechnology/Food & Bio-Industry Research Institute, Kyungpook National University) ;
  • Kim, Ye-Jin (School of Food science & Biotechnology/Food & Bio-Industry Research Institute, Kyungpook National University) ;
  • Ahn, Dong-Hyun (Department of Food Science and Technology, Pukyong National University) ;
  • Cho, Young-Je (School of Food science & Biotechnology/Food & Bio-Industry Research Institute, Kyungpook National University)
  • Received : 2017.03.03
  • Accepted : 2017.03.30
  • Published : 2017.06.30

Abstract

The extracted phenolic compounds from Pinus densiflora root were examined biological activities for beauty food. The tyrosinase inhibitory activity which was related to skin-whitening was observed. The tyrosinase inhibitory activity was confirmed to be 92% in ethanol extract at $50{\mu}g/mL$ phenolic. The elastase and collagenase inhibitory activity as anti-wrinkle effect were showed 61 and 78% in ethanol extract at $200{\mu}g/mL$ phenolic, respectively. Astringent effect of ethanol extract was showed to be 82% at $50{\mu}g/mL$ phenolic. Hyaluronidase inhibitory activity of ethanol extract as anti-inflammation effect was confirmed to be 94% of inhibition at $200{\mu}g/mL$ phenolic. These results demonstrated that isolated phenolic compounds from P. densiflora root could be expected to use as a functional cosmetic materials.

소나무 뿌리(동송근)로부터 phenolic compounds를 추출 후 미용식품활성을 검정하여 기능성 소재로 활용가능성을 살펴보았다. 동송근에 함유된 페놀성 물질의 tyrosinase 저해효과를 측정한 결과 $50{\mu}g/mL$ phenolics 농도에서 ethanol 추출물이 92%의 tyrosinase 저해효과를 나타내었다. Elastase와 collagenase 저해효과를 측정한 결과 $200{\mu}g/mL$ phenolics 농도에서 ethanol 추출물이 각각 61, 78%의 높은 저해력을 나타내어 주름개선효과가 높았다. 수렴효과는 $50{\mu}g/mL$ phenolics의 저농도 ethanol 추출물에서 82%의 저해효과를 나타내었다. 염증억제효과를 나타내는 hyaluronidase 저해효과를 측정한 결과 $100-200{\mu}g/mL$ phenolic 농도의 ethanol 추출물에서 74-94%의 매우 높은 염증억제효과를 나타내었다. 이러한 결과로 보아 동송근 추출물이 미용식품활성 또는 기능성 소재로서 활용이 가능할 것으로 판단되었다.

Keywords

References

  1. Barrantes E, Guinea M (2003) Inhibition of collagenase and metalloproteinases by aloins and aloe gel. Life Sci 72: 843-850 https://doi.org/10.1016/S0024-3205(02)02308-1
  2. Boo YC, Jean CO, Oh JY (1994) Isolation of 4-hydroxy-5-methyl-3[2H]-furanone from pine needles as an antioxidative principle. J Korean Soc Agric Chem Biotechnol 37: 310-314
  3. Cho YJ (2012) Charactrization of biological activities of Rehmannia glutinosa extracts. Journal of Life Science 22: 943-949 https://doi.org/10.5352/JLS.2012.22.7.943
  4. Cho YJ (2014) Biological activity of extracts from Chrysanthemum incidicum Linne by ultrafine grinding. J Korean Soc Food Sci Nutr 43: 110-117 https://doi.org/10.3746/jkfn.2014.43.1.110
  5. Cho YJ, AN BJ (2008) Anti-inflammatory effect of extracts from Cheongmoknosang (Morus alba L.) in Lipopolysaccharide stimulated Raw cells. J Kor Soc Appl Biol Chem 51: 44-48
  6. Demina NS, Lysenko SV (1996) Collagenolytic enzymes synthesized by microorganisms. Mikrobiologiia 65: 293-304
  7. Dewitt DL, Rollins TE, Day JS, Gauger JA, Smith WL (1981) Orientation of the active site and antigenic determinants of prostaglandin endoperoxide of synthase in the endoplasmic reticulum. J Biol Chem 256: 10375-10382
  8. Dorfman A, Ott ML (1948) A turbidimetric method for the assay of hyaluronidase. Journal of biological chemistry 172: 367-375
  9. Elias PM (1983) Epidermal lipids, barrier function, and desquamation. Journal of Invest Dermatol 80: 44-49 https://doi.org/10.1111/1523-1747.ep12531034
  10. Feingold KR (1997) Permeability barrier homeostasis: its biochemical basis and regulation. Cosmet Toilet 112: 49-59
  11. Folin O, Denis W (1912) On phosphotungstic-phosphomolybdic compounds as colcor reagents. J Biol Chem 12: 239-243
  12. Ghosh P (1994) The role of hyaluronic acid (hyaluronan) in health and disease: interactions with cells, cartilage and components of synovial fluid. Clin Exp Rheumatol 12: 75-82
  13. Grant NH, Alburn HE (1959) Studies on the collagenases of Clostridium histolyticum. Arch Biochem Biophys 82: 245-255 https://doi.org/10.1016/0003-9861(59)90120-1
  14. Halliwell B, Gutteridge JM (1990) Role of free radical and catalytic metal ions in human disease, an overview. Methods Enzymol 186: 1-85
  15. Jeroma SP, Gabrielle L, Raul F (1998) Identification of collagen fibrils in scleroderma skin. J Invest Dermatol 90: 48-54
  16. Joung KH (2010) Physicochemical characteristics of dasik using Scatteredflower ladybell(Adenophora remotiflora). Dissertation, Kongju National University
  17. Kwak YJ, Lee DH, Kim NM, Lee JS (2005) Screening and extraction condition of anti-skin aging elastase inhibitor from medicinal plants. Kor J Med Crop Sci 3: 213-216
  18. Kim IK, Shin SR, Chung JH, Youn KS, Kim KS (1997) Changes on the components of dongchimi added ginseng and pine needle. Korean J Food Sci Technol 9: 153-160
  19. Kim MJ, Kim JY, Choi SW, Hong JT, Yoon KS (2004) Anti-wrinkle effect of safflower(Cathamus tinctotius) seed extract. J Kor Soc Cosmetic Sci 30: 15-22
  20. Kim MR, Hwang JH, Yun JK, Han KH, Do EJ, Lee JS, Lee EJ, Kim JB (2011) Antioxidation and anti-aging effect of mixed extract from Korean medicinal herbs. Kor J Herbology 26: 111-117
  21. Kim SR, Yoo TS (2005) The relationships between the extent of $women^{\circ}Os$ skin care by clothing behavior and self-efficacy. J Kor Soc Cloth Ind 7: 413-418
  22. Kim YS, Park JY (2009) The analysis on appearance management of male college students: focused on management of hair, skin, cosmetic surgery, fashion and body shape. Korean J Human Ecol 18: 259-273 https://doi.org/10.5934/KJHE.2009.18.1.259
  23. Kraunsoe JAE, Claridge TDW, Lowe G (1996) Inhibition of human leukocyte and porcine pancreatic elastase by homologues of bovine pancreatic trypsin inhibitor. Biochemistry 35: 9090-9096 https://doi.org/10.1021/bi953013b
  24. Laskin JD, Piccinini LA (1986) Tyrosinase isozyme heterogeneity in differentiating B-16/C3 melanoma. J Biol Chem 261: 16626-16635
  25. Lee EH, Kim NH, Park MJ, Hong EJ, Park KT, An BJ, Ahn DH, Cho YJ (2016) Functional food activities of extracts from Pinus densiflora root. Korean J Food Preserv 23: 110-116 https://doi.org/10.11002/kjfp.2016.23.1.110
  26. Lee JT, Jeong YS, An BJ (2002) Physiological activity of Salicornia herbacea and its application for cosmetic materials. Kor J Herbology 17: 51-60
  27. Lee SH, Chung HS, Lew W (1995) Epidermal lipid homeostasis. Ann Dermatology 7: 99-111 https://doi.org/10.5021/ad.1995.7.2.99
  28. Lee SH, Lee KO, Kim SO (2009) Effects of skin care on users' mental health and self-esteem. J Kor Soc Cosm 15: 155-167
  29. Lee TW, Kim SN, Jee UK, Hwang SJ (2004) Anti-wrinkle effect of pressure sensitive adhesive hydrogel patches containing Ulmi cortex extract. J Kor Pharm Sci 34: 193-199
  30. Lee YH, Shin YM, Cha SH, Choi YS, Lee SY (1996) Development of the health foods containing the extract from Pinus strobus leave. J Korean Soc Food Sci Nutr 25: 379-383
  31. Lim YS, Bae MJ, Lee SH (2002) Antimicrobial effects of Pinus densiflora Sieb. et Zucc. ethanol extract on Listeria monocytogenes. J Korean Soc Food Sci Nutr 31: 333-337 https://doi.org/10.3746/jkfn.2002.31.2.333
  32. Oh YA, Choi KH, Kim SD (1997a) Changes in enzymes activities and growth of lactic acid bacteria in pine needle added kimchi during fermentation. J Food Sci Technol 9: 75-84
  33. Oh YA, Choi KH, Kim SD (1998) Changes in enzyme activities and population of lactic acid bacteria during the kimchi fermentation supplemented with water extract of pine needle. J Korean Soc Food Sci Nutr 27: 244-251
  34. Oh YA, Sae KY, Kim SD (1997b) Quality of pine needle added kimchi. J Food Sci Technol 9: 51-56
  35. Ramarathnam N, Osawa T, Ochi H, Kawakishi S (1995) The contribution of plant food antioxidants to human health. Trends Food Science 6: 75-82 https://doi.org/10.1016/S0924-2244(00)88967-0
  36. Rotts R (1997) Skin barrier: Principles of percutaneous absorption. Hans Schaefer; Thomas E. Redelmeier. Archives of dermatology 133: 924
  37. Tsuji N, Moriwaki S, suzuki Y, Takema Y, Imokawa G (2001) The role of elastases secreted by fibroblasts in wrinkle formation: implication through selective inhibition of elastase activity. Photochem Photobiol 74: 283-290 https://doi.org/10.1562/0031-8655(2001)074<0283:TROESB>2.0.CO;2
  38. Vincent J, Hearing JR (1987) Mammalian monophenol monooxygenase (tyrosinase): Purification, properties, and reactions catalyzed. Method Enzymol 142: 154-165
  39. Wunsch E, Heidrich HG (1963) Zur quantitativen bestimmung der kollagenase. Hoppe-Seyler's Physiol chem 333: 149-151 https://doi.org/10.1515/bchm2.1963.333.1.149