Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation of salt-tolerant bacteria from rhizosphere and rhizoplane of halophyte plant Suaeda japonica in Gochang·Buan tidal flat

고창·부안 갯벌에 자생하는 염생식물 칠면초 근권 및 근면으로부터 내 염성 세균 분리와 그 특성화

  • Ki, Min-Gyu (Department of Bioenvironmental Chemistry, Chonbuk National University) ;
  • Lee, Hyeri (Department of Bioenvironmental Chemistry, Chonbuk National University) ;
  • Cho, Ahyeon (Department of Bioenvironmental Chemistry, Chonbuk National University) ;
  • Unno, Tatsuya (Faculty of Biotechnology, Jeju National University) ;
  • Lee, Ji-Hoon (Department of Bioenvironmental Chemistry, Chonbuk National University)
  • Received : 2016.12.29
  • Accepted : 2017.04.09
  • Published : 2017.06.30
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Abstract

Nine strains of high concentrations of salt-tolerant bacteria were isolated from the rhizosphere and rhizoplane of the halophyte plant Suaeda japonica grown in Gochang Buan tidal flat. The isolated bacteria were classified as genera Vibrio (strains JRS-1, -2, -3, -4, and -5, and JRL-1 and -4) and Bacillus (strains JRL-2 and -3) based on the 16S rRNA gene sequence similarity. The optical growth condition for salt concentration was examined on the selected, representative strains. Strain JRS-1 with the closest relative of Vibrio neocaledonicus showed the highest growth rate at the total salt concentration of 6% among the incubation conditions of 3-8% salt concentrations. Strain JRL-2 with the closest relative of Bacillus thuringiensis showed the tendency that growth rate increased with increasing salt concentrations and the maximum growth rate at 7% of the total salt concentration. The isolated bacteria showed salt-resistances to higher salt concentrations than their habitat soils with 3%. In addition, we identified evidences of potentially plant interaction-relevant enzymatic activities, from utilization of some substrates rich in plants, such as triglyceride, ${\rho}$-nitrophenyl-${\alpha}$,$\text\tiny{D}$-glucoside, and ${\rho}$-nitrophenyl-${\beta}$,$\text\tiny{D}$-glucoside.

고창 부안 갯벌에 자생하는 칠면초의 근권 및 근면으로부터 내염성을 지니는 9 균주를 분리하였다. 16S rRNA 유전자를 이용한 계통분석으로 분리된 균주는 Vibrio 속과 Bacillus 속으로 분류되었다. 분리된 균주 중 Vibrio와 Bacillus로 나누어 각각 대표성을 나타낼 수 있는 균주를 선택하여 염 농도에 따른 최적 생장조건을 평가한 결과, 균주 JRS-1 (Vibrio neocaledonicus)은 총 염분 4-6%에서 높은 생장률을 보였다. 균주 JRL-2(Bacillus thuringiensis)는 염분 농도 증가에 따라 생장이 증가하였으며, 7%에서 최대 생장률을 보이고 8%에서는 감소하는 경향을 보였다. 분리된 균주들은 기존에 서식하던 토양의 염 농도인 3% (w/v) 보다 더 높은 염분에도 견딜 수 있는 내염성을 보였다. 또한 분리균주에 대한 생화학적 기질 이용 형태에 분석을 통해 triglyceride, ${\rho}$-nitrophenyl-${\alpha}$,$\text\tiny{D}$-glucoside, ${\rho}$-nitrophenyl-${\beta}$, $\text\tiny{D}$-glucoside 이용 등 식물과의 특이적인 상호작용을 나타내는 것으로 간주할 수 있는 반응들을 확인하였다.

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