Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

The Accelerating Action of Lipid Excretion of Immature Citrus Fruits

미숙감귤의 지질배설 촉진작용

  • Kim, Ki Jung (The Department of Oriental Medical Food & Nutrition, Semyung University) ;
  • Ko, Sung Kwon (The Department of Oriental Medical Food & Nutrition, Semyung University)
  • 김기중 (세명대학교 한방식품영양학부) ;
  • 고성권 (세명대학교 한방식품영양학부)
  • Received : 2017.06.03
  • Accepted : 2017.06.20
  • Published : 2017.06.30
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Abstract

In a series of investigations to develop a potential anti-obese agent, we prepared a immature Citrus fruits (IMF) and compared its lipid excretion effects to those of Citrus fruits (MF, CP, JP) in an ICR mouse model of obesity induced by a high fat diet. The body weights of IMF fed mice were found to be significantly lower from 2 weeks oral administration, despite the fact that their food intakes were similar to that of the HFD control mice. The fecal cholesterol content showed a significant increase in IMF (52.0 mg/g) at 7 weeks oral administration, and mature Citrus fruits (MF, 41.3 mg/g), immature Citrus peel (CP, 32.2 mg/g), mature Citrus peel (JP, 30.0 mg/g) respectively. And also, the triglyceride content in the feces showed a significant increase in MF (14.6 mg/g), and IMF (14.2 mg/g), CP (10.5 mg/g) and JP (10.0 mg/g), respectively. On the other hand, in the inhibition activity of pancreatic lipase, MF (0.78 nmol/min/ml) showed the greatest decrease in glycerol, CP (0.79 nmol/min/ml), JP, (0.93 nmol/min/ml) and IMF (1.42 nmol/min/ml).

Keywords

References

  1. Ko, S. K. (2016) Herbal edible botany, 189-189, Jinyoungsa, Seoul.
  2. Namba, T. (1993) The encyclopedia of Wakan-Yaku with color pictures. (1), 257-260, Hoikusha, Osaka.
  3. Horowitz, R. and Gentili, B. (1977) Flavonoid constituents of citrus. In Citrus Science and Technology, Nagy., Shaw, P.E and Vedhuis, M.K.(Eds). 397-426, AVI Publishing, Westport.
  4. Lee, M. H., Huh, D., Jo, D. J., Lee, G. D. and Yoon, S. R. (2007) Flavonoids components and functional properties of Citrus peel hydrolysate. J. Korean Soc. Food Sci. Nutr. 36: 1358-1364. https://doi.org/10.3746/jkfn.2007.36.11.1358
  5. Monfore, M. T., Trovato, A., Kirjavainen, S., Foresieri, A. M. and Galat, E. M. (1995) Biological effects of hesperidin, a citrus flavonoid(note II): Hypolipidemic activity on experimental hypercholesterolemia in rat. Farmaco, 50: 595-599.
  6. Bok, S. H., Lee, S. H., Park, Y. B., Bae, K. H., Son, K. H., Jeong, T. S. and Choi, M. S. (1999) Plasma and hepatic cholesterol and hepatic activities of 3-hydroxy-3-methyl-glutarl Coa reductase and acyl Coa: cholesterol transferase are lower in rat fed Citrus peel extract or a mixture of Citrus bioflavonids. J. Nutr. 129: 1182-1185. https://doi.org/10.1093/jn/129.6.1182
  7. Kawaii, S., Tomono, Y., Katase, E., Ogawa, K. and Yano, M. (1999) Quantization of flavonoid constituents in Citrus fruits. J. Agri. Food Chem. 47: 3656-3571. https://doi.org/10.1021/jf981319u
  8. Sen, S., De, B., Devanna, N. and Chakraborty, R. (2013) Total Phenol, total flavonoid content, and antiocidant capacity of the leaves of Meyna spinosa Roxb., an Indian medicinal plant. Chin. J. Nat. Med. 11: 149-157.
  9. Choudhary, R. K. and Swarnkar, P. L. (2011) Antioxidant activity of phenolic and flavonoid in some medicinal plants of India. Nat. Prod. Res. 25: 1101-1109. https://doi.org/10.1080/14786419.2010.498372
  10. Sak, K. (2014) Dependence of DPPH radical scavenging activity of dietary flavonoid quercetin on reaction environment. Mini. Rev. Med. Chem. 14: 494-504. https://doi.org/10.2174/1389557514666140622204037
  11. Matias, A., Nunes, S. L., Poeio, J., Mecha, E., Serra, A. T., Madeira, P. J., Bronze, M. R. and Duarte, C. M. (2014) Antioxidant and anti-inflammatory activity of a flavonoid-rich concentrate recovered from Opuntia ficus-indica juice. Food Funct. 5: 3269-3280. https://doi.org/10.1039/C4FO00071D
  12. Desta, K. T., Kim, G. S., Hong, G. E., Kim, Y. H., Lee, W. S., Lee, S. J., Jin, J. S., El-Aty, A. M., Shin, H. C., Shim, J. H. and Shin, S. C. (2015) Dietary-flavonoid-rich flowers of Rumex nervosus Vahl: Liquid chromatography with electrospray ionization tandem mass spectrometry profiling and in vitro anti-inflammatory effects. J. Sep. Sci. 38: 3345-3353. https://doi.org/10.1002/jssc.201500737
  13. Matias, A., Nunes, S. L., Poeio, J., Mecha, E., Serra, A. T., Madeira, P. J., Bronze, M. R. and Duarte, C. M. (2014) Antioxidant and anti-inflammatory activity of a flavonoid-rich concentrate recovered from Opuntia ficus-indica juice. Food Funct. 5: 3269-3280. https://doi.org/10.1039/C4FO00071D
  14. Hendra, R., Ahmad, S., Sukari, A., Shukor, M. Y. and Oskoueian, E. (2011) Flavonoid analyses and antimicrobial activity of various parts of Phaleria macrocarpa (Scheff.) Boerl fruit. Int. J. Mol. Sci. 12: 3422-3431. https://doi.org/10.3390/ijms12063422
  15. Pistelli, L., Bertoll, A., Noccioll, C., Mendez, J., Musmanno, R. A., Di, Maggio. T. and Coratza, G. (2009) Antimicrobial activity of lnga fendleriana extracts and isolated flavonoids. Nat. Prod. Commun. 4: 1679-1683.
  16. Janssen, C. l., Zerbi, V., Mutsaers, M. P., Jochems, M., Vos, C. A., Vos, J. O., Berg, B. M., van, Tol. EA., Gross, G., Jouni. Z. E., Heerschap, A. and Kiliaan, A. J. (2015) Effect of perinatally supplemented flavonoids on brain structure, circulation, cognition, and metabolism in C57BL/6J mice. Neurochem. Int. 89: 157-169. https://doi.org/10.1016/j.neuint.2015.05.002
  17. Bernatoniene, J., Trumbeckaite, S., Majiene, D., Baniene, R., Baliutyte, G., Savickas, A. and Toleikis, A. (2009) The effect of Crataegus fruit extract and some of its falvonoids on mitochondrial oxidative phosphorylation in the heart. Phytother. Res. 23: 1701-1707. https://doi.org/10.1002/ptr.2815
  18. Yang, X., Yang, J., Xu, C., Huang, M., Zhou, Q., Lv, J., Ma, X., Ke, C., Ye, Y., Shu, G. and Zhao, P. (2015) Antidiabetic effect of flavonoids from Sophora Flavescens EtOAc extract in type 2 diabetic KKAy mice. J. Ethnopharmacol. 171: 161-170. https://doi.org/10.1016/j.jep.2015.05.043
  19. Chen, F., Xiong, H., Wang, J., Ding, X., Shu, G. and Mei, Z. (2013) Antidiabetic effect of total flavonoids from Sanguis draxonis in type 2 diabetic rats. J. Ethnopharmacol. 149: 729-736. https://doi.org/10.1016/j.jep.2013.07.035
  20. Zeng, L. J., Chen, D., Huang, Q. D., Huang, Q., Lian, Y. F., Cai, W. W., Zeng, H. P. and Lin, Y. L. (2015) Isolation of a new flavanone from Daidai fruit and hypolipidemic activity of total flavonoids extracts. Nat. Prod. Res. 29: 1521-1528. https://doi.org/10.1080/14786419.2014.992344
  21. Kim, M. K. and Chung, H. S. (1997) Effect of dietary fibers isolated from tangerine peels on lipid and cadmium metabolism in the rat. Nutraceut. Food. 30: 229-243.
  22. Chung, S. K., Kim, S. H., Choi, Y. H., Song, E. Y. and Kim, S. H. (2000) Status of Citrus fruit production and view of utilization in cheju. Food Ind. Nutr. 5: 42-52.
  23. Son, H. S., Kim, H. S., Kwon, T. B. and Ju, J. S. (1992) Isolation, purification and hypotensive effects of bioflavonoids in Citrus sinensis. J. Korean Soc. Food Nutr. 21: 136-142.
  24. Lee, Y., Howard, L. R. and Villalon, B. (1995) Flavonoids and antioxidant activity of fresh peppera(Capsicum annuaum) cultivars. J. Food Sci. 60: 473-476. https://doi.org/10.1111/j.1365-2621.1995.tb09806.x
  25. Suzuki, R., Kohno, H., Yasui, Y., Hata, K., Sugie, S., Miyamoto, S., Sugaware, K., Sumida, T., Hirose, Y. and Tanaka, T. (2007) Diet supplemented with Citrus unshiu segment membrane suppresses chemically induced colonic preneoplasitic lesions and fatty liver in male db/db mice. Int. J. Cancer. 120: 252-258. https://doi.org/10.1002/ijc.22240
  26. Tanaka, T., Yasui, Y., Ishigamori-Suauki, R. and Oyama, T. (2008) Citrus compounds inhibit inflammation and obesityrelated colon carcingenesis in mice. Nutr. Cancer. 1: 70-80.
  27. Takayanagi, K., Morimoto, S., Shirakura, Y., Mukai, K., Sugiyama, T., Tokuji, Y. and Ohnishi, M. (2011) Mechanism of visceral fat reduction in Tsumura Suzuki obese, diabetes(TSOD) mice orally administered ${\beta}$-cryptoxanthin from Satsuma mandarin orange(Citrus unshiu Marc). J. Agric. Food Chem. 59: 12342-12351. https://doi.org/10.1021/jf202821u
  28. Takayanagi, K. (2011) Prevention of adiposity by the oral administration of ${\beta}$-cryptoxanthin. Front Neurol. 23: 67-67.
  29. George, A. B. and Louis, A. T. (2000) Medicinal strategies in the treatment of obesity. Nature, 404: 672-677. https://doi.org/10.1038/35007544
  30. Yun, S. N., Ko, S. K., Lee, K. H. and Chung, S. H. (2007) Vinegar-processed ginseng radix improves metabolic syndrome induced by a high fat diet in ICR mice. Arch. Pharm. Res. 30: 587-595. https://doi.org/10.1007/BF02977653
  31. Folch, Jordi., Lees, M. and Stanley, G. H. Sloane. (1957) A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226: 497-509.
  32. Charles, C.. Allain, Lucy. S. Poon., Cicely, S. G. Chan., W, Richmond. and Paul, C. Fu. (1974) Enzymatic determination of total serum cholesterol. Clin. Chem. 20: 470-475.
  33. Michael, W., McGowan, Joseph. D. Artiss. and Bennie, Zak. (1983) Enzymatic colorimetry of lecithin and sphingomyelin in aqueous solution. Clin. Chem. 29: 1513-1517.
  34. Matsuda, H., Yano, Maki., Kubo, M., Inuma, M., Oyama, M. and Mizuno, M. (1991) Pharmacological study on Citrus fruits. II. Anti-allergic effect of fruit of Citrus unshiu MARKOVICH (2). On flavonoid components. Yakugaku Zasshi, 111: 193-198. https://doi.org/10.1248/yakushi1947.111.3_193
  35. Nugroho, A., Park, M. G., Jin, S. E., Choi, J. S. and Park, H. J. (2009) Quantitative analysis of flavanone glycosides and peroxynitrite scavenging effect of the five oriental medicinal drugs(Aurantii nobilis Percarpium, Citrii unshiu Percarpium, Citrii unshiu Semen, Aurantii Fructus, Poncirii Fructus). Kor. J. Pharmacogn. 40: 370-375.