• Title/Summary/Keyword: Immature

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Effect of Yellowing Times During Yellowing Stage on Physico-chemical Properties of Immature Tobacco Leaves in Bulk Curing (Bulk건조시 미숙엽의 황변시간 조절이 잎담배 이화학성에 미치는 영향)

  • 이철환;진정의;한철수;이승철
    • Journal of the Korean Society of Tobacco Science
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    • v.21 no.1
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    • pp.10-16
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    • 1999
  • Leaves harvested were separated with visual characters into 2 classes such as immature and mature leaves. In the curing process, the prolonged yellowing treatment during yellowing stage was automatically controlled at the different stalk position, and condition of curing process after this period was all the same with conventional ones. In case of prolonged yellowing in immature leaves, increase of price per kg reached to 8 % compared with those of conventional ones. In physical properties, filling capacity and shatter index was decreased with the degree of maturity, and it was equal level in filling capacity of immature leaves between curing method, while shatter index was decreased in prolonged yellowing treatment than that of conventional ones. There was no difference in chemical components between immature leaves of prolonged yellowing and conventional ones. As to the prolonged yellowing of immature leaves, there was decreased in citric and malic acid contents of the nonvolatile organic acids, and it was equal level in all higher fatty acids content of leaves cured by prolonged yellowing treatment compared with in that of conventional curing method. The contents of key compounds such as solanone, damascenone, damascone in the essential oil were lower in prolonged yellowing of immature leaves than those of mature leaves cured by conventional ones.

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Varietal Difference of Immature Spike Number and Its Relationship with Other Characters in Barley and Wheat (보리ㆍ밀 미열 이삭의 품종간 차이와 주요형질과의 상관)

  • 김흥배
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.2
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    • pp.245-249
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    • 1995
  • The number of immature spikes of barley (Hordeum vulgare L.) and wheat (Triticum aestivum L) were studied to determine if there could be existed the significant difference among 5 varieties of barley and wheat each. Heritability of 6 characters including the number of immature spikes have also calculated. Correlation between the immature spikes and 5 other characters were also investigated. There were significant difference among 5 varieties of barley and wheat in the number of immature spikes. Heritability estimated were very high in case of number of immature spikes of barley and that of wheat was high. There were highly significant correlation between the number of immature spikes of barley and number of internode and significant correlation between the number of immature spikes and flag leaf. However, in wheat no correlations were found among the 5 characters in wheat and between the number of immature spikes and number of internode were negative.

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Clinical Review and PCNA Expressions of Teratomas in Pediatric Patients (소아 기형종의 임상적 고찰 및 PCNA 발현율에 관한 연구)

  • Joo, J.H.;Choi, S.J.N.;Chung, S.Y.;Kim, S.K.
    • Advances in pediatric surgery
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    • v.1 no.2
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    • pp.122-132
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    • 1995
  • Teratoma contains elements derived from all three embryonic germ layers and mature teratomas are entirely composed of mature differentiated tissues, while immature types always contain additional embryonic tissues of variable degree of immaturity, especially neuroepithelial elements. Twenty cases of teratoma in infancy and childhood were reviewed and the clinical features and pathologic findings including PCNA expression were studied. Sacrococcygeal teratomas were the most common type(45%), followed by retroperitoneal and ovarian teratomas. There was a predilection of females in a ratio of 4:1 and age distribution was most prevalent below the first year of age(45%). But in ovarian teratomas, the age at diagnosis was above 4 years of age in all cases. Serum alpha-fetoprotein levels were checked in 18 cases. In all mature teratomas and 1 of 5 immature teratomas, the levels were normal. But in 4 of 5 immature teratomas, the serum levels were elevated and progressively declined to normal range after mass excision. Radiologically, calcifications in tumor were found in 60.0% of teratomas and was higher in mature teratomas(69.2%) than immature teratomas(42.9%). Immunohistochemical staining for PCNA(proliferating cell nuclear antigen) was done in 16 cases and PCNA expression was higher in grade III immature teratomas than grade I and II. The operative modes were complete mass excisions. Five immature teratomas were treated with multiagent PEB(Bleomycin, Etoposide, Cisplatin) adjuvant chemotherapy, 3 tolerated well without significant complications, but in one case, severe bone marrow suppression was developed and died of sepsis. In conclusion, grade III immature teratoma showed higher PCNA expression than mature or lower grade immature teratoma, which suggests that chemotherapy after surgical excision may be effective modality for grade III immature teratoma. We think, however, multicenter study is necessary because of low incidence of immature teratoma.

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Studies on the In-Vitro Fertilization and Survival Rate of Rapidly Frozen Porcine Immature Embryos (돼지 미성숙 난자의 급속동결 융해후 체외수정율과 생존율에 관한 연구)

  • 오원진;정영호;김상근;이규승
    • Korean Journal of Animal Reproduction
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    • v.18 no.1
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    • pp.25-29
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    • 1994
  • This study was carried out to investigate the in-vitro fertilization rate survival rate of rapidly frozen porcine immature embryos. The porcine embryos following dehydration by cryoprotectants containing sucrose were directly plunged into liquid nitrogen and thawed in 3$0^{\circ}C$ water bath. Survival rate was defined as development rate on vitro culture or FDA test. The results are summarized as follows : 1. The in vitro fertilization rate of all frozen immature oocytes(6.7~26.7%) was very low, 35.0% of unfrozen oocytes and the rate of immature oocytes was very higher than that of mature oocytes. 2. The survival rate of all frozen immature oocytes(10.3~25.0%, 13.3~30.0%) was very low, 45% of unfrozen oocytes and the rate of immature oocytes was slightly higher than that of mature oocytes.

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Anti-Inflammatory Effect of Quercetagetin, an Active Component of Immature Citrus unshiu, in HaCaT Human Keratinocytes

  • Kang, Gyeoung-Jin;Han, Sang-Chul;Ock, Jong-Woo;Kang, Hee-Kyoung;Yoo, Eun-Sook
    • Biomolecules & Therapeutics
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    • v.21 no.2
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    • pp.138-145
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    • 2013
  • Citrus fruit contain various flavonoids that have multiple biological activities. However, the content of these flavonoids are changed during maturation and immature Citrus is known to contain larger amounts than mature. Chemokines are significant mediators for cell migration, while thymus and activation-regulated chemokine (TARC/CCL17) and macrophage-derived chemokine (MDC/CCL22) are well known as the typical inflammatory chemokines in atopic dermatitis (AD), a pruritic and chronic inflammatory skin disease. We reported recently that the EtOH extract of immature Citrus unshiu inhibits TARC and MDC production. Therefore, we investigated the activity of flavonoids contained in immature Citrus on TARC and MDC levels. As a result, among the various flavonoids, quercetagetin has stronger inhibitory effects on the protein and mRNA expression of TARC and MDC than other flavonoids. Quercetagetin particularly has better activity on TARC and MDC level than quercetin. In HPLC analysis, the standard peak of quercetagetin matches the peaks of extract of immature C. unshiu. This suggests that quercetagetin is an anti-inflammatory component in immature C. unshiu.

Freezing and In Vitro Fertilization of Porcine Oocytes (돼지난포란의 동결과 체외수정에 관한 연구)

  • 이장희;김창근;정영채
    • Korean Journal of Animal Reproduction
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    • v.21 no.4
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    • pp.355-362
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    • 1997
  • This study was undertaken in an effort to product embryos through in vitro maturation(IVM), in vitro fertilization(IVF) and in vitro culture(IVC) after cryopreservation of immature and mature porcine oocytes. The experiments were conducted to investigate IVM rate of oocytes frozen with 3 different cryoprotectants and to examine IVF and IVC of frozen-thawed oocytes. The CEI(cumulus cells expansion index) after IVM of frozen-thawed immature oocytes was higher in oocytes frozen with PG+PEG(propylene glycol plus polyethylene glycol) than those frozen with single cryoprotectant and this index was almost 90% of unfrozen oocyte's index(2.39 vs. 2.66). The IVF rate of all frozen oocytes was very low(68% of unfrozen oocytes) and the IVF rate of frozen immature oocytes was slightly higher than that of frozen mature oocytes(39.0% vs. 34.4%), but polyspermic penetration was higher in frozen immature oocytes(21.9% vs. 19.1%). The cleavage rate after IVF of frozen-thawed oocytes was 9.3% for frozen mature oocytes and 11.3% for frozen immature oocytes and this rate was significantly lower(P<0.05) than that of control(60.7%). The development to 8-cell stage was greatly lower in frozen mature oocytes than in frozen immature oocytes. The results indicate that the use of PG plus PEG as cryoprotectant may be very effective for vitrification of porcine oocytes and the frozen-thawed immature porcine oocytes can be used fro in vitro embryo production based on IVM, IVF and IVC system.

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Properties of Immature Green Cherry Tomato Pickles (미숙 청방울 토마토 피클 제조 조건에 관한 연구)

  • Koh, Jong-Ho;Shin, Hae-Hun;Kim, Young-Shik;Kook, Moo-Chang
    • The Korean Journal of Food And Nutrition
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    • v.25 no.1
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    • pp.77-82
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    • 2012
  • This study was carried out to optimize the production of immature green cherry tomato pickles and to produce green cherry tomato pickles of good sensory quality. The composition of immature green cherry tomato pickles was optimized using a central composition design with 3 variables and 3 levels. The overall acceptability score, based on sensory evaluation, was best, when the immature green cherry tomato pickles contained 231 g of vinegar, 52.6 g of salt, 168.3 g of sugar, 204 g of tomatoes, and 231 $m{\ell}$ of water. The statically predicted optimal formulation of immature green cherry tomato pickles on overall acceptability value was 33.54%(w/w) of vinegar, 7.64%(w/w) of salt, 25.28%(w/w) of sucrose, 33.54%(w/w) of water. The optimal conditions for producing immature green cherry tomato pickles should consider the factors of time and temperature of storage.

Effects of Spermine on Quisqualate-induced Excitotoxicity in Rat Immature Cortical Neurons (흰쥐 미숙 대뇌피질 신경세포에서 Quisqualate로 유발된 흥분성 세포독성에 대한 spermine의 영향)

  • 조정숙
    • YAKHAK HOEJI
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    • v.43 no.4
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    • pp.535-540
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    • 1999
  • Glutamate (Glu) receptor-mediated excitoxicity has been implicated in many acute and chronic types of neurological disorders. Exposure of mature rat cortical neurons (15-18 days in culture) to the various concentrations of Glu resulted in a marked neuronal death, whereas immature rat cortical neurons (4∼5 days in culture) were resistant to the Glu-induced toxicity. Glu receptor subtype-specific agonists showed differential extent of toxicity in the immature neurons. The neurons treated with NMDA or kainate (KA) did not exhibit damage. However, quisqualate (QA) treatment induced a considerable cell death (36.1%) in immature enurons. The non-NMDA antagonist DNQX did not reduce this response. Interestingly, the QA-induced toxicity was potentiated by spermine in a concentration-dependent manner. Again, the spermine-enhanced damage was not altered by the polyamine antagonist ifenprodil. Taken together, unlike NMDA or KA, QA can induce neurotoxicity in immature rat cortical neurons and the QA-induced toxicity was potentiated by spermine. The lack of antagonizing effects of DNQX and ifenprodil on QA-induced toxicity and the potentiated toxicity by spermine, respectively, implies that both QA receptor and the polyamine site of NMDA receptor may not mediate the neurotoxicity observed in this study, and that a distinct mechanism(s) may be involved in excitotoxicity in immature neurons.

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Immature Stages of Korean Thiaspida Weise (Coleoptera: Chrysomelidae) (한국산 큰남생이잎벌레속(Thlaspida)의 미성숙 단계에 관한 연구(딱정벌레목: 잎벌레과))

  • 이종은;박중직
    • The Korean Journal of Zoology
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    • v.39 no.4
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    • pp.387-392
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    • 1996
  • All immature stages, eggs, larvae, and pupae of Korean Thiaspida, T. cribrosa and T. lewisii, are described and Illustrated for the first time. Two species belonging to genus Thiaspida In the subfamily Cassidlnae; T. cribrosa and T. lewisii.Observatiors on bionomics of immature stages and on their taxonomic charaders are also given.

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