Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Insect Pest Control Technique Using dsRNA

dsRNA를 이용한 해충방제 기술

  • Kim, Yonggyun (Department of Plant Medicals, Andong National University)
  • Received : 2017.02.25
  • Accepted : 2017.04.15
  • Published : 2017.06.01
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Abstract

Gene silencing using double-stranded RNA (dsRNA) has been widely used in functional genomics in biological organisms. Its principle stems from RNA interference (RNAi), a post-transcriptional control of gene expression. Suppression of specific gene expression using dsRNA may give significant lethal effect. Insect pest control exploits this molecular process to develop novel insecticides using specific dsRNAs. This review explains core principles of RNAi using dsRNA. Then it illustrates various examples to control insect pests using dsRNAs. It also discusses limitations to control insect pests using dsRNAs. Finally, it provides several breakthroughs to develop dsRNA insecticides.

이중나선형 RNA (dsRNA)를 이용한 유전자 발현 억제기술이 다양한 생명체에서 기능 유전체학을 연구하는 데 이용되고 있다. 이 기술의 원리는 전사후 단계에서 유전자 발현을 조절하는 RNA 간섭에 기인된다. dsRNA를 이용하여 특정 유전자의 발현 억제는 심각한 치사효과를 줄 수 있다. 이러한 분자기작을 해충 방제에 적용하여 특정 dsRNA를 이용한 새로운 살충제를 개발하고 있다. 본 종설은 dsRNA를 이용한 RNA간섭 원리를 설명하고 이를 이용한 해충 방제를 구현한 여러 예를 살펴본다. 그리고 해충방제를 실현시키기 위해 현재 이 기술이 담고 있는 한계를 고찰하고 이에 대한 대응 방안을 본 종설에서 제공하고자 한다.

Keywords

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