Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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Analysis of the shelf life of chitosan stored in different types of packaging, using colorimetry and dentin microhardness

  • Received : 2016.07.19
  • Accepted : 2016.10.19
  • Published : 2017.05.31
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Abstract

Objectives: Chitosan has been widely investigated and used. However, the literature does not refer to the shelf life of this solution. This study evaluated, through the colorimetric titration technique and an analysis of dentin micro-hardness, the shelf life of 0.2% chitosan solution. Materials and Methods: Thirty human canines were sectioned, and specimens were obtained from the second and third slices, from cemento-enamel junction to the apex. A 0.2% chitosan solution was prepared and distributed in 3 identical glass bottles (v1, v2, and v3) and 3 plastic bottles (p1, p2, and p3). At 0, 7, 15, 30, 45, 60, 90, 120, 150, and 180 days, the specimens were immersed in each solution for 5 minutes (n = 3 each). The chelating effect of the solution was assessed by micro-hardness and colorimetric analysis of the dentin specimens. 17% EDTA and distilled water were used as controls. Data were analyzed statistically by two-way and Tukey-Kramer multiple comparison (${\alpha}=0.05$). Results: There was no statistically significant difference among the solutions with respect to the study time (p = 0.113) and micro-hardness/time interaction (p = 0.329). Chitosan solutions and EDTA reduced the micro-hardness in a similar manner and differed significantly from the control group (p < 0.001). Chitosan solutions chelated calcium ions throughout the entire experiment. Conclusions: Regardless of the storage form, chitosan demonstrates a chelating property for a minimum period of 6 months.

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References

  1. Uragami T, Yamamoto S, Miyata T. Dehydration from alcohols by polyion complex cross-linked chitosan composite membranes during evapomeation. Biomacromolecules 2003;4:137-144. https://doi.org/10.1021/bm025642o
  2. Senel S, Kas HS, Squier CA. Application of chitosan in dental drug delivery and therapy. In: Muzzarelli RAA, editor. Chitosan per os: from dietary supplement to drug carrier. Grottammare, Italy: Atec; 2000. p241-256.
  3. Akncbay H, Senel S, Ay ZY. Application of chitosan gel in the treatment of chronic periodontitis. J Biomed Mater Res B Appl Biomater 2007;80:290-296.
  4. Dash M, Chiellini F, Ottenbrite RM, Chiellini E. Chitosan - a versatile semi-synthetic polymer in biomedical applications. Prog Polym Sci 2011;36:981-1014. https://doi.org/10.1016/j.progpolymsci.2011.02.001
  5. Jeon YJ, Shahidi F, Kim SK. Preparation of chitin and chitosan oligomers and their applications in physiological functional foods. Food Rev Int 2000;16:159-176. https://doi.org/10.1081/FRI-100100286
  6. Zhang A, Mu H, Zhang W, Cui G, Zhu J, Duan J. Chitosan coupling makes microbial biofilms susceptible to antibiotics. Sci Rep 2013;3:3364. https://doi.org/10.1038/srep03364
  7. Muzzarelli RA, Mattioli-Belmonte M, Tietz C, Biagini R, Ferioli G, Brunelli MA, Fini M, Giardino R, Ilari P, Biagini G. Stimulatory effect on bone formation exerted by a modified chitosan. Biomaterials 1994;15:1075-1081. https://doi.org/10.1016/0142-9612(94)90093-0
  8. Park SS, Kim SG, Lim SC, Ong JL. Osteogenic activity of the mixture of chitosan and particulate dentin. J Biomed Mater Res A 2008;87:618-623.
  9. Walsh AM, Sweeney T, Bahar B, O'Dohert JV. Multifunctional roles of chitosan as a potential protective agent against obesity. PLoS One 2013;8:e53828. https://doi.org/10.1371/journal.pone.0053828
  10. Park JS, Choi SH, Moon IS, Cho KS, Chai JK, Kim Ck. Eight-week histological analysis on the effect of chitosan on surgically created one-wall intrabony defects in beagle dogs. J Clin Periodontol 2003;30:443-453. https://doi.org/10.1034/j.1600-051X.2003.10283.x
  11. Boynuegri D, Ozcan G, Senel S, Uc D, Uraz A, Ogus E, Cakilci B, Karaduman B. Clinical and radiographic evaluations of chitosan gel in periodontal intraosseous defects: a pilot study. J Biomed Mater Res B Appl Biomater 2009;90:461-466.
  12. Ballal NV, Shavi GV, Kumar R, Kundabala M, Bhat KS. In vitro sustained release of calcium ions and pH maintenance from different vehicles containing calcium hydroxide. J Endod 2010;36:862-866. https://doi.org/10.1016/j.joen.2009.12.021
  13. DaSilva L, Finer Y, Friedman S, Basrani B, Kishen A. Biofilm formation within the interface of bovine root dentin treated with conjugated chitosan and sealer containing chitosan nanoparticles. J Endod 2013;39:249-253. https://doi.org/10.1016/j.joen.2012.11.008
  14. Kim JS, Shin DH. Inhibitory effect on streptococcus mutans and mechanical properties of the chitosan containing composite resin. Restor Dent Endod 2013;38:36-42. https://doi.org/10.5395/rde.2013.38.1.36
  15. Arancibia R, Maturana C, Silva D, Tobar N, Tapia C, Salazar JC, Martinez J, Smith PC. Effects of chitosan particles in periodontal pathogens and gingival fibroblasts. J Dent Res 2013;92:740-745. https://doi.org/10.1177/0022034513494816
  16. Costa EM, Silva S, Madureira AR, Cardelle-Cobas A, Tavaria FK, Pintado MM. A comprehensive study into the impact of a chitosan mouthwash upon oral microorganism's biofilm formation in vitro. Carbohydr Polym 2014;101:1081-1086. https://doi.org/10.1016/j.carbpol.2013.09.041
  17. Kurita K. Chemistry and application of chitin and chitosan. Polym Degrad Stabil 1998;59:117-120. https://doi.org/10.1016/S0141-3910(97)00160-2
  18. Spano JC, Silva RG, Guedes DF, Sousa-Neto MD, Estrela C, Pecora JD. Atomic absorption spectrometry and scanning electron microscopy evaluation of concentration of calcium ions and smear layer removal with root canal chelators. J Endod 2009;35:727-730. https://doi.org/10.1016/j.joen.2009.02.008
  19. Pimenta JA, Zaparolli D, Pecora JD, Cruz-Filho AM. Chitosan: effect of a new chelating agent on the microhardness of root dentin. Braz Dent J 2012;23:212-217. https://doi.org/10.1590/S0103-64402012000300005
  20. Silva PV, Guedes DF, Nakadi FV, Pecora JD, Cruz-Filho AM. Chitosan: a new solution for removal of smear layer after root canal instrumentation. Int Endod J 2013;46:332-338. https://doi.org/10.1111/j.1365-2591.2012.02119.x
  21. Silva PV, Guedes DF, Pecora JD, da Cruz-Filho AM. Timedependent effects of chitosan on dentin structures. Braz Dent J 2012;23:357-361. https://doi.org/10.1590/S0103-64402012000400008
  22. Calt S, Serper A. Time-dependent effects of EDTA on dentin structures. J Endod 2002;28:17-19. https://doi.org/10.1097/00004770-200201000-00004
  23. Hulsmann M, Heckendorff M, Lennon A. Chelating agents in root canal treatment: mode of action and indications for their use. Int Endod J 2003;36:810-830. https://doi.org/10.1111/j.1365-2591.2003.00754.x
  24. Guibal E, Milot C, Eterradossi O, Gauffier C, Domard A. Study of molybdate ion sorption on chitosan gel beabs by different spectometric analyses. Int J Biol Macromol 1999;24:49-59. https://doi.org/10.1016/S0141-8130(98)00067-1
  25. Guibal E, Milot C, Roussy J. Influence of hydrolysis mechanisms on molybdate sortion isotherms using on chitosan. Sep Sci Technol 2000;35:1021-1038. https://doi.org/10.1081/SS-100100208
  26. Carvalho TS, Lussi A. Combined effect of a fluoride-, stannous- and chitosan-containing toothpaste and stannous-containing rinse on the prevention of initial enamel erosion-abrasion. J Dent 2014;42:450-459. https://doi.org/10.1016/j.jdent.2014.01.004
  27. Wang J, Chen C. Chitosan-based biosorbents: modification and application for biosorption of heavymetals and radionuclides. Bioresour Technol 2014;160:129-141. https://doi.org/10.1016/j.biortech.2013.12.110
  28. del Carpio-Perochena A, Bramante CM, Duarte MA, de Mora MR, Aouada FA, Kishen A. Chelating and antibacterial properties of chitosan nanoparticles on dentin. Restor Dent Endod 2015:40;195-201. https://doi.org/10.5395/rde.2015.40.3.195
  29. Rinaudo M. Chitin and chitosan: properties and applications. Prog Polym Sci 2006;31:603-632. https://doi.org/10.1016/j.progpolymsci.2006.06.001
  30. Li Q, Dunn ET, Grandmaison EW, Goosen MFA. Applications and properties of chitosan. J Bioact Compat Polym 1992;7:370-397. https://doi.org/10.1177/088391159200700406
  31. Rhazi M, Desbrieres J, Tolaimate A, Rinaudo M, Vottero P, Alagui A, Meray MEL. Influence of the nature of the metal ions on the complexation with chitosan. Application to the treatment of liquid waste. Eur Polym J 2002;38:1523-1530. https://doi.org/10.1016/S0014-3057(02)00026-5
  32. Nogueira JMF, Serodio P, Agudo R. Validacao de estabilidade de solucoes. Bol Soc Port Quim 2001;81:51-53.
  33. Silva BCB, Irala LED, Grazziotin-Soares R, Salles AA. Verification of structural changes of glass when submitted to 17% EDTA. Rev Bras Odontol 2011;8:43-48.
  34. Vogel AI. Quantitative chemical analysis. 6th ed. Rio de Janeiro: Ltc SA; 2002. p26-27.

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