Parasites, Hosts and Diseases

  • 제55권2호
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  • Pages.115-120
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  • 2017
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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DNA Methylation of Gene Expression in Acanthamoeba castellanii Encystation

  • Moon, Eun-Kyung (Department of Medical Zoology, Kyung Hee University School of Medicine) ;
  • Hong, Yeonchul (Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine) ;
  • Lee, Hae-Ahm (Department of Pharmacology, Kyungpook National University School of Medicine) ;
  • Quan, Fu-Shi (Department of Medical Zoology, Kyung Hee University School of Medicine) ;
  • Kong, Hyun-Hee (Department of Parasitology, Dong-A University College of Medicine)
  • 투고 : 2017.02.21
  • 심사 : 2017.04.03
  • 발행 : 2017.04.30
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초록

Encystation mediating cyst specific cysteine proteinase (CSCP) of Acanthamoeba castellanii is expressed remarkably during encystation. However, the molecular mechanism involved in the regulation of CSCP gene expression remains unclear. In this study, we focused on epigenetic regulation of gene expression during encystation of Acanthamoeba. To evaluate methylation as a potential mechanism involved in the regulation of CSCP expression, we first investigated the correlation between promoter methylation status of CSCP gene and its expression. A 2,878 bp of promoter sequence of CSCP gene was amplified by PCR. Three CpG islands (island 1-3) were detected in this sequence using bioinformatics tools. Methylation of CpG island in trophozoites and cysts was measured by bisulfite sequence PCR. CSCP promoter methylation of CpG island 1 (1,633 bp) was found in 8.2% of trophozoites and 7.3% of cysts. Methylation of CpG island 2 (625 bp) was observed in 4.2% of trophozoites and 5.8% of cysts. Methylation of CpG island 3 (367 bp) in trophozoites and cysts was both 3.6%. These results suggest that DNA methylation system is present in CSCP gene expression of Acanthamoeba. In addition, the expression of encystation mediating CSCP is correlated with promoter CpG island 1 hypomethylation.

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참고문헌

  1. Aksozek A, McClellan K, Howard K, Niederkorn JY, Alizadeh H. Resistance of Acanthamoeba castellanii cysts to physical, chemical, and radiological conditions. J Parasitol 2002; 88: 621-623. https://doi.org/10.1645/0022-3395(2002)088[0621:ROACCT]2.0.CO;2
  2. Turner NA, Russell AD, Furr JR, Lloyd D. Emergence of resistance to biocides during differentiation of Acanthamoeba castellanii. J Antimicrob Chemother 2000; 46: 27-34. https://doi.org/10.1093/jac/46.1.27
  3. Moon EK, Xuan YH, Chung DI, Hong Y, Kong HH. Microarray analysis of differentially expressed genes between cysts and trophozoites of Acanthamoeba castellanii. Korean J Parasitol 2011; 49: 341-347. https://doi.org/10.3347/kjp.2011.49.4.341
  4. Moon EK, Chung DI, Hong YC, Kong HH. Autophagy protein 8 mediating autophagosome in encysting Acanthamoeba. Mol Biochem Parasitol 2009; 168: 43-48. https://doi.org/10.1016/j.molbiopara.2009.06.005
  5. Song SM, Han BI, Moon EK, Lee YR, Yu HS, Jha BK, Danne DB, Kong HH, Chung DI, Hong Y. Autophagy protein 16-mediated autophagy is required for the encystation of Acanthamoeba castellanii. Mol Biochem Parasitol 2012; 183: 158-165. https://doi.org/10.1016/j.molbiopara.2012.02.013
  6. Moon EK, Hong Y, Chung DI, Goo YK, Kong HH. Down-regulation of cellulose synthase inhibits the formation of endocysts in Acanthamoeba. Korean J Parasitol 2014; 52: 131-135. https://doi.org/10.3347/kjp.2014.52.2.131
  7. Moon EK, Chung DI, Hong YC, Kong HH. Characterization of a serine proteinase mediating encystation of Acanthamoeba. Eukaryot Cell 2008; 7: 1513-1517. https://doi.org/10.1128/EC.00068-08
  8. Moon EK, Hong Y, Chung DI, Kong HH. Cysteine protease involving in autophagosomal degradation of mitochondria during encystation of Acanthamoeba. Mol Biochem Parasitol 2012; 185: 121-126. https://doi.org/10.1016/j.molbiopara.2012.07.008
  9. Portela A, Esteller M. Epigenetic modifications and human disease. Nat Biotechnol 2010; 28: 1057-1068. https://doi.org/10.1038/nbt.1685
  10. Robertson KD. DNA methylation and chromatin - unraveling the tangled web. Oncogene 2002; 21: 5361-5379. https://doi.org/10.1038/sj.onc.1205609
  11. Attwood JT, Yung RL, Richardson BC. DNA methylation and the regulation of gene transcription. Cell Mol Life Sci 2002; 59: 241-257. https://doi.org/10.1007/s00018-002-8420-z
  12. Goto T, Monk M. Regulation of X-chromosome inactivation in development in mice and humans. Microbiol Mol Biol Rev 1998; 62: 362-378.
  13. Fisher O, Siman-Tov R, Ankri S. Characterization of cytosine methylated regions and 5-cytosine DNA methyltransferase (Ehmeth) in the protozoan parasite Entamoeba histolytica. Nucleic Acids Res 2004; 32: 287-297. https://doi.org/10.1093/nar/gkh161
  14. Ponts N, Fu L, Harris EY, Zhang J, Chung DW, Cervantes MC, Prudhomme J, Atanasova-Penichon V, Zehraoui E, Bunnik EM, Rodrigues EM, Lonardi S, Hicks GR, Wang Y, Le Roch KG. Genome-wide mapping of DNA methylation in the human malaria parasite Plasmodium falciparum. Cell Host Microbe 2013; 14: 696-706. https://doi.org/10.1016/j.chom.2013.11.007
  15. Geyer KK, Rodriguez Lopez CM, Chalmers IW, Munshi SE, Truscott M, Heald J, Wilkinson MJ, Hoffmann KF. Cytosine methylation regulates oviposition in the pathogenic blood fluke Schistosoma mansoni. Nat Commun 2011; 2: 424. https://doi.org/10.1038/ncomms1433
  16. Moon EK, Hong Y, Chung DI, Goo YK, Kong HH. Identification of protein arginine methyltransferase 5 as a regulator for encystation of Acanthamoeba. Korean J Parasitol 2016; 54: 133-138. https://doi.org/10.3347/kjp.2016.54.2.133
  17. Bernes S, Siman-Tov R, Ankri S. Epigenetic and classical activation of Entamoeba histolytica heat shock protein 100 (EHsp100) expression. FEBS Lett 2005; 579: 6395-6402. https://doi.org/10.1016/j.febslet.2005.09.101
  18. Jones PA, Liang G. Rethinking how DNA methylation patterns are maintained. Nat Rev Genet 2009; 10: 805-811.
  19. Lim JH, Kim SP, Gabrielson E, Park YB, Park JW, Kwon TK. Activation of human cancer/testis antigen gene, XAGE-1, in tumor cells is correlated with CpG island hypomethylation. Int J Cancer 2005; 116: 200-206. https://doi.org/10.1002/ijc.21007

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