Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Gene Expression Analysis from the Normal Stomach Cells Treated with a Cancer Inducer N-methyl-N'-nitro-N-nitrosoguanidine, MNNG

  • Jung, Dongju (Biomedical Science Institute, College of Life and Health Sciences, Hoseo University) ;
  • Cho, Yoonjung (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Kim, Tae Ue (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Jeong, Sang-Hee (Biomedical Science Institute, College of Life and Health Sciences, Hoseo University)
  • Received : 2016.11.29
  • Accepted : 2017.02.02
  • Published : 2017.03.31
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Abstract

N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) is a carcinogen made of modified guanine on which alkyl group is added on 6th oxygen. It has been used for inducing different types of cancers experimentally in vivo and in vitro. Stomach cancer might be the best well established particular cancer induced with MNNG. Comparative analysis of gene expression between normal stomach cell and MNNG-treated stomach cell could give much information to understand cancer formation in stomach. To this end, normal human stomach cells HS738 were treated with DMSO or MNNG. Genetic comparison was conducted with purified RNA from the treated cells for 6 hours or 24 hours. Total 13 genes were selected based on their high induction folds and comprehensible function to cancer formation. Some of the genes were cancer-promoting whereas the others were anti-cancer genes. These results could give important information of genetic changes in stomach cells during MNNG-induced stomach cancer formation.

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References

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